FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489803

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489803
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences490346
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN47240.9634013533300975No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12610.2571653485497995No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA11710.238810962055365No Hit
GTTTTAGTACAATATGTTCTTGCGGTAAAGTACCCCAACTTCCAATTACA11340.23126526982987522No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG11280.2300416440635796No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT11250.22942983118043178No Hit
GTACTAAAACTTAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT10460.21331875859087254No Hit
GTACAAAAACTTAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT10260.2092400060365538No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9970.20332581483279155No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT9330.19027380665897142No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT9210.18782655512638013No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8570.17477454695256003No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCATAT8310.1694721686319456No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC8240.16804460523793405No Hit
CTCCAAAAGATACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGA7980.1627422269173196No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7720.15743984859670518No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC7600.1549925970641139No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC7420.15132171976522701No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTATTCCCTTAACTTCAT7190.1466311543277604No Hit
GTCTTACATAAGAGGACTCTTGGACTCTTACATAAGAGGACTCTTGGACT7110.14499965330603287No Hit
GTTTTTGTACAATATGTTCTTGCGGTAAAGTACCCCAACTTCCAATTACA6810.1388815244745547No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6780.13826971159140689No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTACTTCCGAGAGAGGACAACAGAGT6760.137861836335975No Hit
GCACAATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA6590.13439489666480403No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6470.13194764513221277No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA6370.12990826885505335No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCCCGGA6300.1284807054610418No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6280.12807283020560992No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA6270.12786889257789397No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG6250.12746101732246212No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6150.1254216410453027No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6000.12236257662956362No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5940.12113895086326798No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATCCG5940.12113895086326798No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5910.12052713798012014No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA5860.11950744984154046No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA5850.11930351221382453No Hit
CTCTTGGACTCTCAGCAATGTCAACGACCGACCTTGAGGCATACTTCAAA5770.11767201119209701No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC5680.11583657254265356No Hit
TTTTAGTACAATATGTTCTTGCGGTAAAGTACCCCAACTTCCAATTACAT5650.11522475965950574No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC5620.11461294677635793No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTATTCCCTTAAC5590.1140011338932101No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5550.11318538338234634No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA5190.10584362878457251No Hit
GGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT5150.10502787827370878No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5130.1046200030182769No Hit
CTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC5100.10400819013512907No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCCCGGATAGAAGGAAA5070.10339637725198125No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5060.1031924396242653No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5030.1025806267411175No Hit
GTTTCGCTCCAGACCGGCTGCGAGCAAAACAAGCTGCTAGGAGTTCCGCA5030.1025806267411175No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4960.10115306334710593No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT4950.10094912571938998No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA4950.10094912571938998No Hit
GTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTTAAGGACT4940.10074518809167406No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA4930.1005412504639581No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT4910.10013337520852622No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)