FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489804

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489804
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492019
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGTGTGTTCAGG37320.7585072934175306No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN35090.7131838404614457No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG21370.43433282048050986No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT18360.3731563211989781No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAGTTGATTATGCCTGCTAGG18090.3676687282401696No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT16430.3339301937526803No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGTCCCACAATTCGTTGACATACT14240.2894197175312336No Hit
GGATAATAAGGTTTAATGCCTTTATCTAGGGGCAAATATTTAGTGCGGTT12410.2522260319215315No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGTGTGTTCA12100.24592546222808467No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAGTTGA11870.24125084600391447No Hit
GTTCTATCCTAACCGCACTAAATATTTGCCCCTAGATAAAGGCATTAAAC11790.2396248925346379No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA11740.23860867161634003No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT11690.23759245069804213No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT11580.23535676467778682No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAGTTGATTATG11240.2284464624333613No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAAT11090.22539779967846768No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAA10980.22316211365821237No Hit
ATCCTAACCGCACTAAATATTTGCCCCTAGATAAAGGCATTAAACCTTAT10710.2176745206994039No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC10460.21259341610791452No Hit
CTCTTATATAAAATACCCGCCTTCCACAGAGTATGTAAATAATGCCTAGT10310.2095447533530209No Hit
GGATAGAACCTAGCAGGCATAATCAACTTTAATCTCCTTTTTTCGTTAAC10230.20791879988374434No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA10180.20690257896544645No Hit
ATGATTAACTGTGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAGGG10160.2064960905981273No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCATTGACATTGCTGAGAGTCCAA10070.20466689294519114No Hit
CTTATATAAAATACCCGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT9890.20100849763931883No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGTG9310.1892203349870635No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9220.18739113733412735No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT9040.18373274202825501No Hit
GATTAACTGTGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAGGGGC8920.18129381182434012No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC8900.18088732345702097No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCTCATTCATTTGAAAG8890.1806840792733614No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC8790.17865163743676565No Hit
TATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAA8780.17844839325310607No Hit
CTTCTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCTC8430.17133484682502098No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8160.1658472538662125No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC8050.16361156784595718No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC7840.1593434399891061No Hit
CAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGA7700.15649802141787206No Hit
CAATAGGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGT7630.15507531213225506No Hit
CCTTTATCTAGGGGCAAATATTTAGTGCGGTTAGGATAGAACCTAGCAGG7420.150807184275404No Hit
GTTTGGAAGTAATGATTAACTGTGTGTTCAGGATAATAAGGTTTAATGCC7400.15040069590808486No Hit
ATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAA7330.14897798662246783No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG7280.14796176570416997No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTTCTGTACCTGTC7260.1475552773368508No Hit
ATATAAAATACCCGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGA7160.14552283550025505No Hit
AGATAAAGGCATTAAACCTTATTATCCTGAACACACAGTTAATCATTACT7100.14430337039829763No Hit
TTATTATCCTGAACACACAGTTAATCATTACTTCCAAACTAGGCATTATT7080.14389688203097847No Hit
CAATATGTTCTTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATG7060.14349039366365934No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAACTTTAATCTCCTTTTTTCG7040.14308390529634019No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAAATGTATA6950.14125470764340403No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTATAGGAAGTTTCCGAAAAC6760.13739306815387212No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6560.13332818448068062No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6500.13210871937872318No Hit
ATGTAATTGGAAGTTGGGGTACCTTACCACAAGAACATATTGTACACAAA6430.13068601009310615No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA6410.13027952172578702No Hit
ACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATGAGAGG6350.12906005662382958No Hit
ATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAGTTGATTA6200.12601139386893595No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAATGACCGACCT6180.1256049055016168No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTTCTG6170.12540166131795724No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAGGGGCAAATATTTAGTG6160.12519841713429766No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC6040.12275948693038277No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGACT6040.12275948693038277No Hit
GGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATG6010.12214975437940404No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC6000.12194651019574447No Hit
ATATGTTCTTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA5900.11991406835914872No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT5840.11869460325719129No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA5780.11747513815523385No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGT5710.11605242886961684No Hit
GTTTTAGTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAAC5630.11442647540034023No Hit
TTCCCCCACTGTTTGGCTTTTAGTTATATGGATGATGTGGTATTGGGGGC5540.11259727774740406No Hit
ATTTACATACTCTGTGGAAGGCGGGTATTTTATATAAGAGAGAAACTACT5530.11239403356374449No Hit
GTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTG5530.11239403356374449No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCCTATCAACACTTCCGGA5500.11178430101276576No Hit
AACATATTGTACACAAAATCAAACAATGTTTTCGGAAACTTCCTATAAAT5480.11137781264544662No Hit
ATGTAAGACCTTGGGCAAGACCTGTCGGGCGTTCACGGTGGTCTCCATGC5450.11076808009446791No Hit
TTCTTCTTCTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC5430.11036159172714875No Hit
GAATTAGTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACA5420.11015834754348917No Hit
GCTTTATTCTTCTTCTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCT5420.11015834754348917No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5380.10934537080885087No Hit
CTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTG5290.1075161731559147No Hit
GTACAATATGTTCTTGTGGTAAGGTACCCCAACTTCCAATTACATATCCC5260.106906440604936No Hit
CTATTGATAATATCCTCTTTCAAATGAATGAGAGGAAAAGAGGGAGTTTG5210.1058902196866381No Hit
ATAATATCCTCTTTCAAATGAATGAGAGGAAAAGAGGGAGTTTGCCAGTC5210.1058902196866381No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5210.1058902196866381No Hit
GTGTACAATATGTTCTTGTGGTAAGGTACCCCAACTTCCAATTACATATC4990.10141884764612748No Hit
GTTTCTCTCTTATATAAAATACCCGCCTTCCACAGAGTATGTAAATAATG4980.10121560346246791No Hit
GTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCGC4940.10040262672782961No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)