FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489805

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489805
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492019
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGTGTGTTCAGG40510.8233421880049347No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN35030.7119643753594881No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG20140.4093337858903823No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAGTTGATTATGCCTGCTAGG17630.35831949579182915No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT17010.3457183564049356No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT16890.3432794262010207No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGTCCCACAATTCGTTGACATACT13800.2804769734502123No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA13190.2680790782469783No Hit
GGATAATAAGGTTTAATGCCTTTATCTAGGGGCAAATATTTAGTGCGGTT13080.26584339222672293No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGTGTGTTCA12110.24612870641174425No Hit
GTTCTATCCTAACCGCACTAAATATTTGCCCCTAGATAAAGGCATTAAAC12000.24389302039148894No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT11540.23454378794314854No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT11110.22580428804578687No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAA10700.21747127651574433No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAGTTGA10590.215235590495489No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAGTTGATTATG10400.21137395100595707No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAAT10070.20466689294519114No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC9990.20304093947591453No Hit
CTCTTATATAAAATACCCGCCTTCCACAGAGTATGTAAATAATGCCTAGT9840.19999227672102093No Hit
CTTATATAAAATACCCGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT9740.1979598348844252No Hit
GGATAGAACCTAGCAGGCATAATCAACTTTAATCTCCTTTTTTCGTTAAC9680.19674036978246773No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA9670.1965371255988082No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCATTGACATTGCTGAGAGTCCAA9480.1926754861092763No Hit
ATGATTAACTGTGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAGGG9450.19206575355829755No Hit
GATTAACTGTGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAGGGGC9230.1875943815177869No Hit
ATCCTAACCGCACTAAATATTTGCCCCTAGATAAAGGCATTAAACCTTAT9100.18495220713021246No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC9100.18495220713021246No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCTCATTCATTTGAAAG8960.1821067885589784No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGTG8910.18109056764068054No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8790.17865163743676565No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8620.1751964863145529No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC8530.17336728866161674No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT8440.17153809100868056No Hit
CTTCTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCTC8420.17113160264136143No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC8130.16523752131523375No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC7910.16076614927472313No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG7860.15974992835642526No Hit
GTTTGGAAGTAATGATTAACTGTGTGTTCAGGATAATAAGGTTTAATGCC7840.1593434399891061No Hit
TATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAA7800.15853046325446782No Hit
CAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGA7390.15019745172442528No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAACTTTAATCTCCTTTTTTCG7270.1477585215205104No Hit
CAATATGTTCTTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATG7200.14633581223489336No Hit
CAATAGGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGT7160.14552283550025505No Hit
TTATTATCCTGAACACACAGTTAATCATTACTTCCAAACTAGGCATTATT7020.14267741692902103No Hit
CCTTTATCTAGGGGCAAATATTTAGTGCGGTTAGGATAGAACCTAGCAGG6970.14166119601072316No Hit
AGATAAAGGCATTAAACCTTATTATCCTGAACACACAGTTAATCATTACT6920.1406449750924253No Hit
ATATAAAATACCCGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGA6890.14003524254144656No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTTCTGTACCTGTC6780.13779955652119125No Hit
ATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAA6750.13718982397021254No Hit
ATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAGTTGATTA6740.13698657978655296No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTTCTG6560.13332818448068062No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAAATGTATA6420.13048276590944657No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTATAGGAAGTTTCCGAAAAC6400.13007627754212742No Hit
ACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATGAGAGG6400.13007627754212742No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAGGGGCAAATATTTAGTG6380.1296697891748083No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6340.12885681244017No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA6340.12885681244017No Hit
ATATGTTCTTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA6320.12845032407285084No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCCTATCAACACTTCCGGA6320.12845032407285084No Hit
ATGTAATTGGAAGTTGGGGTACCTTACCACAAGAACATATTGTACACAAA6180.1256049055016168No Hit
GTTTTAGTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAAC6130.12458868458331894No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGT6120.12438544039965935No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGACT6110.12418219621599978No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC6020.12235299856306363No Hit
GGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATG5900.11991406835914872No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5890.11971082417548916No Hit
GAATTAGTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACA5860.11910109162451042No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAATGACCGACCT5770.11727189397157427No Hit
ATTTACATACTCTGTGGAAGGCGGGTATTTTATATAAGAGAGAAACTACT5660.11503620795131894No Hit
GCTTTATTCTTCTTCTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCT5600.1138167428493615No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA5470.11117456846178704No Hit
TTCTTCTTCTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC5450.11076808009446791No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC5440.11056483591080832No Hit
TTCCCCCACTGTTTGGCTTTTAGTTATATGGATGATGTGGTATTGGGGGC5400.10975185917617002No Hit
GTACAATATGTTCTTGTGGTAAGGTACCCCAACTTCCAATTACATATCCC5400.10975185917617002No Hit
ATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGTGTGTTCAGGAT5320.10812590570689344No Hit
AACATATTGTACACAAAATCAAACAATGTTTTCGGAAACTTCCTATAAAT5280.10731292897225514No Hit
GTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTG5270.10710968478859556No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA5160.10487399876834025No Hit
CTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTG5130.10426426621736153No Hit
GGATATGTAATTGGAAGTTGGGGTACCTTACCACAAGAACATATTGTACA5100.10365453366638279No Hit
GTTTCTCTCTTATATAAAATACCCGCCTTCCACAGAGTATGTAAATAATG5050.10263831274808494No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)