FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501290
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25870.5160685431586507No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGTATGTTCCGG21340.4257016896407269No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAACCTTAT17900.357078736858904No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT14770.29463982924055937No Hit
TTATTAATAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATGAAAAA12740.2541443076861697No Hit
GTCTTTGAAGTACGCCTCAAGGTCGGTCGTTGACATCGCTGAGAGTCCAA11370.226814817770153No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT11000.21943386063954995No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA10850.21644158072173791No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG10650.21245187416465522No Hit
GTGTAAAAGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9320.18592032556005506No Hit
CTATTAATAATGTCCTCCTGCAAATGAATGTGAGGAAAGGAGGGAGTTTG8990.17933730974086856No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACATAAATC8350.16657024875820384No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8330.16617127810249555No Hit
CTATATAAGAGAGAAACCACACGCAGCGCCTCATTTTGTGGGTCACCATA8120.1619820862175587No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8010.1597877476111632No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAAC7910.15779289433262184No Hit
CTGTAAGAGGGCCTACAAATTGTTGACATCTATTAATAATGTCCTCCTGC7350.1466217159727902No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT7170.14303098007141576No Hit
GTTTACAGGAAGTTTTCGAAAACATTGCTTAAGTTTTAGTACAATATGTT7030.14023818548145783No Hit
ATATAAGAGAGAAACCACACGCAGCGCCTCATTTTGTGGGTCACCATATT6880.1372459055636458No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC6870.13704642023579164No Hit
CTCCAAAAGACACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGA6840.13644796425222927No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAA6780.13525105228510442No Hit
GGAACATATTGTACTAAAACTTAAGCAATGTTTTCGAAAACTTCCTGTAA6700.13365516966227134No Hit
CCCTTAACTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG6620.13205928703943826No Hit
CCCTTAATTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG6490.12946597777733448No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC6470.12906700712162622No Hit
GTCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATAA6310.12587524187596003No Hit
GGGTAAAGGTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5930.11829479941750284No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGTATGTTCC5870.11709788745037802No Hit
AGTTAAGGGAGTAGCCCCAACGCTTGGTTTTATTAGGGTTTAAATGTACA5860.11689840212252389No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCTACACTTCCGGA5840.11649943146681563No Hit
CTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTG5830.11629994613896147No Hit
CCCTTAGACAAAGGCATTAAACCTTATTATCCGGAACATACAGTTAATCA5810.1159009754832532No Hit
GGTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG5800.11570149015539907No Hit
TTGACATACTTTCCAATCAATAGGTCTGTTTACAGGAAGTTTTCGAAAAC5790.11550200482754493No Hit
CAATAGGTCTGTTTACAGGAAGTTTTCGAAAACATTGCTTAAGTTTTAGT5750.11470406351612839No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGTA5700.1137066368768577No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC5690.11350715154900357No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC5540.11051487163119152No Hit
CCTGTAAACAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT5430.10832053302479604No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA5420.10812104769694188No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTGCAGG5400.10772207704123361No Hit
GTACTAAAACTTAAGCAATGTTTTCGAAAACTTCCTGTAAACAGACCTAT5320.10612619441840053No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC5210.10393185581200502No Hit
ATATTGTACTAAAACTTAAGCAATGTTTTCGAAAACTTCCTGTAAACAGA5170.10313391450058847No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA5170.10313391450058847No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5100.10173751720560953No Hit
GTTTTGAAGTAATGATTAACTGTATGTTCCGGATAATAAGGTTTAATGCC5070.10113906122204712No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5020.10014163458277645No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)