FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489808

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489808
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences574930
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG26580.4623171516532448No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT22880.3979614909641173No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22720.39517854347485787No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG18320.3186474875202198No Hit
GTTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG17660.3071678291270241No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG17130.29794931556885185No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA16920.2942966969891987No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT15790.274642130346303No Hit
GTGTAAAGGGAGCGGCAAACCCCAAAAGACCCACAATTCTTTGACATACT15620.27168524863896476No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12790.22246186492268621No Hit
CCCATAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACACAGTCTTT12400.21567843041761606No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA11850.20611204842328631No Hit
GGAACATATTGTACAAAAAATCAAAAATTGTTTTCGGAAACTTCCCATAA11220.1951541926843268No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG11210.19498025846624806No Hit
GCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCGGTG11110.19324091628546083No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG10820.1881968239611779No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT10590.18419633694536724No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGCACCTTGCCACAG10420.18123945523802898No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC10000.1739342180787226No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATA9970.17341241542448646No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTA9890.17202094167985668No Hit
TTCCCTGGCTGCTCGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC9810.1706294679352269No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG9760.1697597968448333No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9620.16732471779173116No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT9520.16558537561094394No Hit
ATGTAAGACCTTAGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC9440.16419390186631416No Hit
ATATGTTCCTGTGGCAAGGTGCCCCAACTTCCAATTACATATCCCATGAA9300.16175882281321205No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT9130.15880194110587376No Hit
TATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAA8680.15097490129233124No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC8670.1508009670742525No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT8640.15027916442001635No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCA8420.14645261162228446No Hit
ATTTATGGGAAGTTTCCGAAAACAATTTTTGATTTTTTGTACAATATGTT8410.14627867740420572No Hit
ATCCTAAACTTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT8410.14627867740420572No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACGGTACCTGTC8190.14245212460647383No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTAAC8160.14193032195223768No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTATTGCCAATTTTCTT8060.14019097977145042No Hit
CTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCATATTCATTTGAAAG7810.13584262431948238No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7770.13514688744716746No Hit
CCCAGTACAAAGATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTC7710.13410328213869516No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC7700.13392934792061642No Hit
ACTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATA7670.13340754526638027No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT7660.13323361104830153No Hit
CAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAAGGA7530.13097246621327813No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA7510.1306245977771207No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT7490.13027672934096327No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG7430.1292331240324909No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC7370.12818951872401857No Hit
CTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATAT7320.12731984763362497No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG7260.1262762423251526No Hit
TTCCATGGCTGCTCGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC6990.12158001843702712No Hit
GTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTG6820.11862313672968883No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA6820.11862313672968883No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6800.11827526829353138No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA6790.11810133407545266No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTAGGCAAGACCTGGCGGGCGTTC6750.11740559720313777No Hit
ATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAA6740.11723166298505906No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA6670.11601412345850798No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACGG6630.11531838658619312No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC6580.11444871549579948No Hit
GCTTACTACTAATTCCCTGGATGCCGGGTCTTCCAAATTACTTCCCACCC6580.11444871549579948No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTATGGGAAGTTTCCGAAAAC6570.11427478127772077No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGCAATAGCGGTAAAAA6530.11357904440540588No Hit
ATAATATCCTCTTTCAAATGAATATGAGGAAAAGAGGGAGTTTGCCATTC6500.11305724175116971No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG6470.11253543909693353No Hit
GATTAAGACCTTCGTCTGCGAGGCGAGGGAGTTCTTCTTCTAGGGGACCT6420.11166576800653992No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6310.10975249160767397No Hit
GTTTGGAAGTAATGATTAACTATATGTTCAGGATAATAAGGTTTGATGCC6280.10923068895343782No Hit
TTATTATCCTGAACATATAGTTAATCATTACTTCCAAACTAGGCATTATT6260.10888282051728036No Hit
GCTTTATTCTTCTACGGTACCTGTCTTTAATCCTGAATGGCAAACTCCCT6230.10836101786304418No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA6190.1076652809907293No Hit
TTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTC6110.10627380724609953No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT6100.1060998730280208No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT6060.10540413615570592No Hit
GATAATATCCTCTTTCAAATGAATATGAGGAAAAGAGGGAGTTTGCCATT6050.10523020193762718No Hit
CCCATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT6040.10505626771954847No Hit
CAATAGGTCTATTTATGGGAAGTTTCCGAAAACAATTTTTGATTTTTTGT6040.10505626771954847No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5970.10383872819299741No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5950.10349085975683997No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC5940.10331692553876123No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5900.10262118866644636No Hit
CAATATGTGGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGAT5860.10192545179413147No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5850.10175151757605273No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5840.10157758335797402No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT5820.10122971492181657No Hit
CTATTGATAATATCCTCTTTCAAATGAATATGAGGAAAAGAGGGAGTTTG5810.10105578070373783No Hit
GAATTAGTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACA5800.10088184648565912No Hit
CTCCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG5790.10070791226758041No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC5760.10018610961334423No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)