FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences574930
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG30150.5244116675073488No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22620.3934392012940706No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT20280.3527385942636495No Hit
GTTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG18880.3283878037326283No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA18190.31638634268519644No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG17850.31047257927051986No Hit
GTGTAAAGGGAGCGGCAAACCCCAAAAGACCCACAATTCTTTGACATACT16170.2812516306332945No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG15610.271511314420886No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT15020.2612491955542414No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA13670.2377680761136138No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG12870.22385333866731602No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12740.22159219383229264No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC11300.19654566642895657No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG11290.19637173221087784No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG10930.19011010036004383No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGCACCTTGCCACAG10600.18437027116344598No Hit
TTCCCTGGCTGCTCGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC10390.1807176525837928No Hit
GCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCGGTG10090.17549962604143113No Hit
CCCATAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACACAGTCTTT9900.1721948758979354No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTA9780.17010766528099072No Hit
ATATGTTCCTGTGGCAAGGTGCCCCAACTTCCAATTACATATCCCATGAA9710.16889012575443965No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT9500.1652375071747865No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT9460.1645417703024716No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCA9430.16401996764823545No Hit
GGAACATATTGTACAAAAAATCAAAAATTGTTTTCGGAAACTTCCCATAA8960.15584505939853546No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATA8890.15462751987198442No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8610.14975736176578017No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA8500.14784408536691424No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT8440.1468004800584419No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC8420.14645261162228446No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT8290.14419146678726105No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACGGTACCTGTC8260.1436696641330249No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC8190.14245212460647383No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTAAC8180.14227819038839512No Hit
CTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATAT8120.14123458507992276No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT7980.13879950602682067No Hit
ATTTATGGGAAGTTTCCGAAAACAATTTTTGATTTTTTGTACAATATGTT7880.13706016384603342No Hit
CAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAAGGA7810.13584262431948238No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTATTGCCAATTTTCTT7750.13479901901101002No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG7740.1346250847929313No Hit
ATCCTAAACTTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT7660.13323361104830153No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACGG7520.13079853199519942No Hit
TTCCATGGCTGCTCGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC7500.13045066355904197No Hit
TATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAA7500.13045066355904197No Hit
CCCAGTACAAAGATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTC7440.12940705825056964No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA7420.12905918981441217No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT7340.12766771606978242No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC7320.12731984763362497No Hit
ATGTAAGACCTTAGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC6990.12158001843702712No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6970.12123215000086968No Hit
CTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCATATTCATTTGAAAG6960.12105821578279095No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT6790.11810133407545266No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6760.11757953142121651No Hit
GTTTGGAAGTAATGATTAACTATATGTTCAGGATAATAAGGTTTGATGCC6710.11670986033082288No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA6710.11670986033082288No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6670.11601412345850798No Hit
ACTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATA6610.11497051815003566No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6550.11392691284156332No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC6520.11340511018732716No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA6240.10853495208112292No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG6170.10731741255457186No Hit
GTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCATATTCATTTGAA6110.10627380724609953No Hit
GTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTG6050.10523020193762718No Hit
GCTTACTACTAATTCCCTGGATGCCGGGTCTTCCAAATTACTTCCCACCC6050.10523020193762718No Hit
TTATTATCCTGAACATATAGTTAATCATTACTTCCAAACTAGGCATTATT6040.10505626771954847No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA6030.10488233350146973No Hit
ATAATATCCTCTTTCAAATGAATATGAGGAAAAGAGGGAGTTTGCCATTC5990.10418659662915486No Hit
ATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAA5980.10401266241107612No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG5960.10366479397491868No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5960.10366479397491868No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGGCCGACCACG5930.10314299132068253No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC5910.10279512288452507No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGCAATAGCGGTAAAAA5910.10279512288452507No Hit
CCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT5850.10175151757605273No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT5850.10175151757605273No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA5790.10070791226758041No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG5780.10053397804950168No Hit
CAATATGTGGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGAT5750.10001217539526552No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)