FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences580280
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25500.43944302750396363No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT24530.42272695939891086No Hit
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTAACTGTAAATGAAAAGAG23350.40239194871441375No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT14190.24453712001102917No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC13070.225236092920659No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA12810.22075549734610878No Hit
TATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT12500.21541324877645276No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG12050.20765837182050045No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11900.20507341283518302No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT11880.20472875163714072No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC11840.20403942924105603No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGGT11530.1986971806714No Hit
ATATGATCCTGTGGCAAAGTTCCCCAACTTCCAATTATGTAACCCATGAA11310.19490590749293443No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT11060.19059764251740538No Hit
ATTCTATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAAC11040.19025298131936308No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC10980.1892189977252361No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT10140.17474322740745846No Hit
TTATTAATAGGTGTCAACAATTTGTGGGCCCTCTAACTGTAAATGAAAAG10110.17422623561039496No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA10040.17301992141724684No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9620.16578203625835802No Hit
AGTCTGTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT9600.16543737506031572No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGTCACCCGTG9430.16250775487695596No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG8900.15337423312883436No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA8840.15234024953470737No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA7970.13734748741986627No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT7820.13476252843454883No Hit
CTCTAACTGTAAATGAAAAGAGAAGATTGAAATTAATTATGCCTGCTAGA7610.13114358585510444No Hit
CTATATAAGAGGGAAACTACACGTAGCGCATCATTTTGCGGGTCACCATA7420.12786930447370234No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG7380.1271799820776177No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT7160.12338870889915213No Hit
CTCCGAAAGAGACCAAATATTCAAGTACAGTCTCTCTTCCAAAAGTAAGG7100.12235472530502516No Hit
TTCAAGTACAGTCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACA7090.12218239470600399No Hit
ATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGG6770.1166678155373268No Hit
CTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCATTTACAAG6730.11597849314124217No Hit
GTCTAAGGGCAAATATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAA6550.11287654235886124No Hit
GTACAATATGATCCTGTGGCAAAGTTCCCCAACTTCCAATTATGTAACCC6450.11115323636864963No Hit
GTCCCGTACTGGTTGTTGTGGATCCTGGAATTAGAGGACAAACGGGCAAC6370.10977459157648031No Hit
ATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTAT6310.10874060798235334No Hit
CCCTTAGACAAAGGAATTAAACCTTATTATCCAGATCAGGTAGTTAATCA6290.10839594678431101No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC6230.10736196319018405No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6070.10460467360584545No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTGTTTGATCTTTTGT6030.10391535120976081No Hit
GTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA5920.10201971462052802No Hit
TTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTC5900.10167505342248569No Hit
TTCTAAAACAGTGTTTGATCTTTTGTACAATATGATCCTGTGGCAAAGTT5900.10167505342248569No Hit
GGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCA5870.10115806162542221No Hit
CCTCCAGCCTATAGGCCACCAAATGCCCCTATCTTATCAACACTTCCGGA5850.10081340042737989No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)