FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences580280
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25360.43703039911766733No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT23040.3970497001447577No Hit
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTAACTGTAAATGAAAAGAG21920.37774867305438753No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT13010.22420210932653203No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT12460.2147239263803681No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC12220.2105879920038602No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA11990.2066243882263735No Hit
ATATGATCCTGTGGCAAAGTTCCCCAACTTCCAATTATGTAACCCATGAA11470.19766319707727303No Hit
TATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT11390.19628455228510375No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC11230.19352726270076515No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT10930.1883573447301303No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG10510.18111945957124145No Hit
TTATTAATAGGTGTCAACAATTTGTGGGCCCTCTAACTGTAAATGAAAAG10390.1790514923829875No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10300.17750051699179706No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA10300.17750051699179706No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGTCACCCGTG10050.173192252016268No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGGT9940.17129661542703523No Hit
ATTCTATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAAC9830.16940097883780245No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC9640.16612669745640035No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG9610.16560970565933686No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT9580.1650927138622734No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT9390.1618184324808713No Hit
AGTCTGTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT9260.15957813469359622No Hit
CTCCGAAAGAGACCAAATATTCAAGTACAGTCTCTCTTCCAAAAGTAAGG8430.14527469497483972No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG8330.1435513889846281No Hit
CTCTAACTGTAAATGAAAAGAGAAGATTGAAATTAATTATGCCTGCTAGA7360.1268353208795754No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA7280.12545667608740607No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT7240.12476735369132143No Hit
CTATATAAGAGGGAAACTACACGTAGCGCATCATTTTGCGGGTCACCATA7220.12442269249327911No Hit
CTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCATTTACAAG7170.12356103949817329No Hit
GTCTAAGGGCAAATATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAA7120.1226993865030675No Hit
GTACAATATGATCCTGTGGCAAAGTTCCCCAACTTCCAATTATGTAACCC6820.11752946853243262No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA6700.11546150134417867No Hit
CCTCCAGCCTATAGGCCACCAAATGCCCCTATCTTATCAACACTTCCGGA6290.10839594678431101No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT6240.10753429378920522No Hit
ATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTAT6170.10632797959605708No Hit
ATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGG6120.10546632660095126No Hit
TTCAAGTACAGTCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACA6040.10408768180878197No Hit
GTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA6020.10374302061073963No Hit
GTCCCGTACTGGTTGTTGTGGATCCTGGAATTAGAGGACAAACGGGCAAC6000.10339835941269732No Hit
GGGTAAAGGTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA5970.10288136761563384No Hit
GCTTTATTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTT5820.10029640863031639No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)