FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489812

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489812
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences634707
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA58010.9139650263822521No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT23290.3669409664616902No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20110.31683910843901203No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT17480.2754026661120801No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT16610.26169555401153605No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT15860.24987907806279117No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT15690.24720067684774233No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC14500.2284518683424005No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT14050.2213619827731536No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA13680.2155325213051061No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG12620.1988319019642134No Hit
CTATATCACAATGTACTTAATCACCTGTTCATGAACATTTCGTTTCCAGT12040.18969382723051736No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC11950.188275850116668No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT11900.18748808505341835No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC11620.18307660069922027No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC11410.1797679874335717No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT11300.17803490429442245No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT11160.17582916211732344No Hit
GTACTTAATCACCTGTTCATGAACATTTCGTTTCCAGTGTTTCTTCAAAA11160.17582916211732344No Hit
TTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC10900.17173278378842521No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT10650.16779395847217693No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA10260.1616493909788296No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT10220.16101917892822987No Hit
CAATAGGCCTATTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT10160.1600738608523303No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10160.1600738608523303No Hit
GTGTAAAAGGGGCAGCAAACCCCAAAAGACCCACAATACGTTGACAGACT10120.15944364880173056No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG9950.15676524758668173No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT9610.15140844515658405No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC9380.14778472586563562No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG9070.14290058247348777No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA8730.1375437800433901No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT8710.13722867401809025No Hit
GAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCCAAATGTTA8340.13139921255004278No Hit
GGGTATACATTTAAATCCTAACAAAACAAAAAGATGGGGTTACTCTTTAC8300.13076900049944304No Hit
GATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTGG8280.13045389447414318No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC8280.13045389447414318No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT8200.12919347037294374No Hit
GTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCC8000.1260424101199451No Hit
CATTTAAATCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT7990.1258848571072952No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC7900.12446687999344579No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATT7650.12052805467719752No Hit
ATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAG7650.12052805467719752No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT7540.11879497153804827No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7360.1159590173103495No Hit
GTGCTGGAGAAGGAAAAGGGATGAATAGGCAGAATGCACAGGATTTTTAG7340.11564391128504962No Hit
GCTTTATTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCT7090.11170508596880135No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG7020.11060221488025183No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG6840.10776626065255306No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT6780.10682094257665348No Hit
GTTTTATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAAC6740.10619073052605375No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAACCCCAAAAGACCCACAAT6740.10619073052605375No Hit
GTGTATTAGTAGTTCATTCTTTTAATTGCTGAGTAGTGTTCCATTGTATG6720.10587562450075388No Hit
GGATATCTCTGTATCTTTTTCTCAGTTTTGATGTTGAACCTAAAATTGCT6700.10556051847545403No Hit
ATATAGAGCTGAGGCGGTATCTAGAAGATCCCGTACTGAAGGAAAGAAGT6650.10477275341220438No Hit
GTTATGTTAACACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTT6600.10398498834895471No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG6590.10382743533630479No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA6550.10319722328570506No Hit
CTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTA6530.1028821172604052No Hit
CTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC6510.10256701123510534No Hit
TTGTATGACTATATCACAATGTACTTAATCACCTGTTCATGAACATTTCG6420.10114903412125596No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6420.10114903412125596No Hit
TCCTAGTACAAAGACCTTTAATCTAGTCTCCTCCCCCAACTCCTCCCAGT6400.10083392809595608No Hit
CCTTTAATCTAGTCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAG6360.10020371604535636No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)