FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489813

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489813
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences634707
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA57820.9109715191419032No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT21670.34141737841240133No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20090.31652400241371215No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT16630.2620106600368359No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT16230.25570853953083866No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT15810.24909131299954151No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT15710.24751578287304218No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC14270.2248281490514521No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC13780.21710805143160544No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT13610.2144296502165566No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA13380.2108059309256082No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG13250.20875774176115908No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC12870.20277072728046172No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT12090.19048159229376704No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC11330.17850756333237225No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT11200.17645937416792315No Hit
CTATATCACAATGTACTTAATCACCTGTTCATGAACATTTCGTTTCCAGT11150.1756716091046735No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA11060.1742536319908241No Hit
GTGTAAAAGGGGCAGCAAACCCCAAAAGACCCACAATACGTTGACAGACT10640.167636405459527No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT10480.1651155572571281No Hit
TTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC10440.16448534520652835No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT10370.16338247411797885No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9510.14983291503008475No Hit
CAATAGGCCTATTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT9450.14888759695418516No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG9410.14825738490358542No Hit
GTACTTAATCACCTGTTCATGAACATTTCGTTTCCAGTGTTTCTTCAAAA8990.14164015837228833No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG8840.13927686318253935No Hit
GGGTATACATTTAAATCCTAACAAAACAAAAAGATGGGGTTACTCTTTAC8640.1361258029295407No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT8540.1345502728030414No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG8410.13250208363859228No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT8100.12761794024644443No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATT7970.12556975108199533No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT7960.1254121980693454No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT7930.12493953903139558No Hit
GTGTATTAGTAGTTCATTCTTTTAATTGCTGAGTAGTGTTCCATTGTATG7910.12462443300609571No Hit
GATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTGG7870.12399422095549599No Hit
CATTTAAATCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT7600.11974028961394785No Hit
GTGCTGGAGAAGGAAAAGGGATGAATAGGCAGAATGCACAGGATTTTTAG7580.119425183588648No Hit
GAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCCAAATGTTA7560.11911007756334813No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC7420.11690433538624907No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA7300.1150136992344499No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7300.1150136992344499No Hit
ATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAG7290.11485614622179997No Hit
GTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCC7260.11438348718385018No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA7180.11312306308265073No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG7130.11233529801940108No Hit
GTTTTATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAAC7110.11202019199410122No Hit
GCTTTATTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCT7060.11123242693085156No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT6990.11012955584230205No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6940.10934179077905239No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC6760.10650583655135361No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC6760.10650583655135361No Hit
CTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTA6730.10603317751340383No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAACCCCAAAAGACCCACAAT6700.10556051847545403No Hit
GGATATCTCTGTATCTTTTTCTCAGTTTTGATGTTGAACCTAAAATTGCT6690.1054029654628041No Hit
GTTATGTTAACACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTT6440.10146414014655582No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6430.10130658713390589No Hit
ATATTTGCCATTGGATAAGGGTATTAAACCTTATTATCCAGAACATCTAG6400.10083392809595608No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)