FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences612801
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN47510.7752924685175122No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGG26700.4357042498298795No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT14900.2431458173207942No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT14300.23335471058304408No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT13400.21866805047641896No Hit
ACACCAAATATTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATG12440.20300227969601878No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT12340.20137042857306045No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT12320.20104405834846878No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12290.20055450301158123No Hit
AATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTTGTCT11390.1858678429049561No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10070.16432740808190588No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA10040.16383785274501836No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG9620.1569840780285933No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACACCTAGTTAATCATTACT9580.15633133757940995No Hit
TTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA9520.15535222690563494No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC9490.15486267156874745No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAATCTTTGATTTTCTGT9180.14980393308757656No Hit
ACACTAAATATTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATG9050.1476825266277307No Hit
GCTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG9030.14735615640313904No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC9000.14686660106625155No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA8680.1416446774727848No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC8620.1406655667990098No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT8310.1356068283178389No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGGTGTTCTGGAT8290.13528045809324724No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA8270.13495408786865556No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG8250.1346277176440639No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG8240.13446453253176804No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7940.12956897916289303No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA7910.12907942382600548No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT7740.1263052769169763No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAATGACC7720.12597890669238462No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT7390.12059379798662208No Hit
GTGTGAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT7390.12059379798662208No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC7380.12043061287432624No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT7190.11733009574070538No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC7180.11716691062840955No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCACCAATTCTTGCCC7030.11471913394397201No Hit
GTATTAAACCTTATTATCCAGAACACCTAGTTAATCATTACTTCCAAACC7030.11471913394397201No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6970.11374002327019701No Hit
ACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTT6960.11357683815790118No Hit
GGATAACCACATTGTGTGAAAGGGGCAGCAAAACCCAAAAGACCCACAAT6910.11276091259642201No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC6680.10900765501361781No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6520.10639669321688444No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT6420.10476484209392609No Hit
ATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTACTGAAGGAAAGAAGT6390.10427528675703858No Hit
CTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC6360.10378573142015109No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC6340.10345936119555942No Hit
GTACAATGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTA6200.10117476962341772No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT6190.10101158451112188No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCAGGCAACGGCCAGGTC6150.10035884406193855No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)