FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489816

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489816
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences584600
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24440.4180636332535067No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG17310.2960998973657202No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA15760.2695860417379405No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA13680.23400615805679098No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT12750.21809784468012317No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT12590.21536093055080396No Hit
GTGCTGGTGAACTGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC11540.19739993157714678No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCCTAT11030.18867601778994184No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTTCCAATTTTCTT10740.1837153609305508No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC10650.18217584673280876No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC10210.17464933287718096No Hit
CATTTGAACCCTAATAAAACCAAACGGTGGGGCTACTCCCTTAACTTCAT9260.15839890523434827No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA9250.15822784810126583No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG9020.1542935340403695No Hit
CCCTAGCAGCCATGGAAAGGCGGTGTATTTCCGAGAGAGGACAACAGAGT8980.1536093055080397No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG8830.15104344851180296No Hit
TTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCT8760.1498460485802258No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCCAGG8580.1467670201847417No Hit
GTACAAAACCTTCGGACGGAAACTGCACTTGTATTCCCATCCCATCATCT8530.14591173451932946No Hit
GGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG8480.14505644885391722No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC8480.14505644885391722No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTTCCAATTTTCTTTTGTC8410.14385904892234005No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8290.14180636332535068No Hit
GGATAAAACCTGGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC7740.13239822100581594No Hit
TTATTCATAGATGTGAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA7610.1301744782757441No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT7610.1301744782757441No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTAGTACAATA7610.1301744782757441No Hit
CCTTTAAATGAATGTGAGGAAAGGAGGGAGTTTGCCACTCAGGATTAAAG7460.12760862127950734No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG7160.12247690728703388No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7140.12213479302086896No Hit
CTCACAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC7130.12196373588778651No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA7020.12008210742387958No Hit
CTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAAC6880.11768730756072528No Hit
GGATAGAACCTGGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC6850.11717413616147794No Hit
GATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGT6790.11614779336298325No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG6780.11597673622990078No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC6670.11409510776599384No Hit
GGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTAGTACAAT6630.11341087923366405No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6490.11101607937050975No Hit
ATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTAGTACAATATGTT6490.11101607937050975No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC6340.108450222374273No Hit
GTCTTTGAAGTACGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6270.10725282244269586No Hit
AGTTAAGGGAGTAGCCCCACCGTTTGGTTTTATTAGGGTTCAAATGTATA6240.1067396510434485No Hit
CATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA6230.10656859391036605No Hit
GCCTTAATGCCTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTT6220.1063975367772836No Hit
GGATAATAGGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT6180.10571330824495381No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCTCCTCC6050.10348956551488196No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5940.10160793705097503No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG5900.10092370851864524No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)