FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489817

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489817
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences584600
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24290.4154977762572699No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG19040.32569278138898394No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA14710.25162504276428327No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA13720.23469038658912078No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT13190.2256243585357509No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT12370.21159767362299006No Hit
GTGCTGGTGAACTGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9960.17037290455011975No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTTCCAATTTTCTT9800.16763599042080055No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCCTAT9630.1647280191583989No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9420.16113581936366747No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC8860.1515566199110503No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG8770.15001710571330823No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA8640.14779336298323642No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG8590.14693807731782416No Hit
CATTTGAACCCTAATAAAACCAAACGGTGGGGCTACTCCCTTAACTTCAT8550.14625384878549436No Hit
GGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG8500.14539856312008212No Hit
GTACAAAACCTTCGGACGGAAACTGCACTTGTATTCCCATCCCATCATCT8100.13855627779678414No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7960.13616147793362984No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTTCCAATTTTCTTTTGTC7810.13359562093739308No Hit
CCCTAGCAGCCATGGAAAGGCGGTGTATTTCCGAGAGAGGACAACAGAGT7780.13308244953814574No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT7780.13308244953814574No Hit
TTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCT7760.13274033527198084No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCCAGG7620.13034553540882654No Hit
GGATAAAACCTGGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC7590.1298323640095792No Hit
CCTTTAAATGAATGTGAGGAAAGGAGGGAGTTTGCCACTCAGGATTAAAG7310.1250427642832706No Hit
TTATTCATAGATGTGAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA7300.12487170715018815No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC7250.12401642148477592No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTAGTACAATA7230.12367430721861102No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6730.11512145056448854No Hit
CTCACAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC6550.11204242216900444No Hit
GGATAATAGGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT6510.11135819363667465No Hit
GCCTTAATGCCTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTT6490.11101607937050975No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT6430.10998973657201506No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG6390.10930550803968525No Hit
GGATAGAACCTGGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC6260.10708176530961341No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC6170.10554225111187136No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGGCCGACCACG6070.10383167978104688No Hit
GATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGT5960.10195005131713993No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG5960.10195005131713993No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5950.10177899418405748No Hit
GGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTAGTACAAT5890.10075265138556279No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)