FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489818

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489818
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377165
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA13410.3555473068816035No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT12670.33592724669574325No Hit
CTACATGCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATAT11780.31233014728301933No Hit
CATTTAAACCCGCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT9880.26195431707608074No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT8850.23464531438495087No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA8690.23040313920962974No Hit
ATCTTAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG8570.22722150782813888No Hit
CATTTACAGGAGGACATTGTTGATAGATGTAAGCAATTTGTGGGGCCCCT8290.21979770127132686No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA8140.2158206620444633No Hit
GCACTGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG8110.21502525419909058No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC7340.19460978616785757No Hit
AATTAGTTATGCCTGCTAGGTTTTATCCCAATGTTACTAAATATTTGCCC7330.1943446502194No Hit
TAACCAATTTTAGTCTCCTGTTTTCATTTACTGTAAGGGGCCCCACAAAT7250.19222356263173943No Hit
ATCTAAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG7150.1895722031471637No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT7040.18665570771413043No Hit
GTCTTTGAAGTAAGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA6900.18294380443572442No Hit
GTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC6570.17419431813662456No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT6470.17154295865204883No Hit
GTACAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT6470.17154295865204883No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACG6300.16703564752827013No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA6200.1643842880436944No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC6110.16199806450757626No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6000.159081569074543No Hit
GTATAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT5960.15802102528071268No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG5960.15802102528071268No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAA5890.15616507364150967No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG5800.15377885010539155No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG5790.15351371415693396No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC5770.1529834422600188No Hit
CTCCAAAAGAAACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA5740.15218803441464612No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5720.15165776251773097No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT5450.14449909190937651No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG5390.1429082762186311No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAACAG5380.14264314027017355No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTC5330.14131746052788566No Hit
GTAAACACACAGTCTTTGAAGTAAGCCTCAAGGTCGGTCGTTGACATTGC5320.14105232457942812No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG5300.14052205268251294No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT5280.13999178078559782No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA5270.13972664483714023No Hit
CCTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCT5240.13893123699176751No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT5160.13681014940410696No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGTGGGTCACCATATT5120.13574960561027666No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5120.13574960561027666No Hit
CCTGTAAATGAATGTCAGGAAAAGAAGGAGTGCGCCAGTTAGGATTAAAG5080.13468906181644638No Hit
GGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGA4980.13203770233187068No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG4970.1317725663834131No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC4830.1280606631050071No Hit
CATTTAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT4830.1280606631050071No Hit
GCTATTGTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAG4790.1270001193111768No Hit
GAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACA4680.1240836238781435No Hit
ATTTGAAGGAGGACATTGTTGATAGATGTAAGCAATTTGTGGGGCCCCTT4660.12355335198122837No Hit
GTTTGATTTTTTGTACAATATGTTCCTGTGGCAATGTGCCCCAACTCCCA4650.12328821603277079No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4630.12275794413585565No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA4590.12169740034202536No Hit
GTCCAGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC4580.12143226439356782No Hit
CCCTTTTTTCAAAGCCAACTCAGAAAATCCAGATTGGGACCTCAACCCGC4490.11904604085744967No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT4470.1185157689605345No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACACCCGTG4430.11745522516670422No Hit
ATTTACAGGAGGACATTGTTGATAGATGTAAGCAATTTGTGGGGCCCCTT4410.11692495326978908No Hit
GCCTAGCAGCCATGGAAAGGATGTATACTTGCGGGAGAGCACGACAGAAT4410.11692495326978908No Hit
CCTTCAAATGAATGTCAGGAAAAGAAGGAGTGCGCCAGTTAGGATTAAAG4400.1166598173213315No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4390.11639468137287394No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACC4370.11586440947595879No Hit
GAGCACGTAGTTAATCATTACTTCCAGACGAGACATTATTTACACACTCT4350.11533413757904365No Hit
GTCCTGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGCAGTATGGATCGG4320.11453872973367094No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGAAA4300.1140084578367558No Hit
GTTAAGGGAATATCCCCATCTTTTTGTTTTGTGCGGGTTTAAATGTATAC4270.11321304999138308No Hit
CTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT4260.1129479140429255No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGTCTGGA4170.11056169050680736No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC4110.10897087481606194No Hit
GGAACATATTGTACAAAAAATCAAACAGTGCTTTAGAAAACTTCCTGTAA4110.10897087481606194No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCTAAACAGGCTTTTACTTT4070.10791033102223165No Hit
GCAGTGGAGACACAAAGGTTCCACGCATGCGCTGATGGCCCATGGCCAAG4060.10764519507377407No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT4030.10684978722840136No Hit
CAATAGGCCTGTTTACAGGAAGTTTTCTAAAGCACTGTTTGATTTTTTGT3970.10525897153765593No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC3950.10472869964074079No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA3950.10472869964074079No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT3930.10419842774382565No Hit
GCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCCCATGGCCAAG3870.10260761205308022No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA3870.10260761205308022No Hit
GGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA3860.10234247610462265No Hit
GTCTTTGGGTATACATTTAAACCCGCACAAAACAAAAAGATGGGGATATT3810.1010167963623348No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)