FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377165
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT12240.3245264009120677No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA11620.3080879721076982No Hit
CTACATGCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATAT10610.2813092413134835No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA8720.23119854705500245No Hit
CATTTAAACCCGCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT8710.23093341110654486No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA8650.22934259541579943No Hit
ATCTTAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG8590.227751779725054No Hit
CATTTACAGGAGGACATTGTTGATAGATGTAAGCAATTTGTGGGGCCCCT8280.2195325653228693No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT7970.21131335092068457No Hit
GCACTGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG7490.19858682539472114No Hit
AATTAGTTATGCCTGCTAGGTTTTATCCCAATGTTACTAAATATTTGCCC7480.19832168944626358No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC7460.19779141754934843No Hit
ATCTAAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG7070.18745111555950314No Hit
GTATAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT6730.1784364933119457No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC6620.17551999787891243No Hit
GTACAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT6540.17339891029125185No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA6410.1699521429613034No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG6390.16942187106438827No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT6280.16650537563135498No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT6070.16093752071374595No Hit
TAACCAATTTTAGTCTCCTGTTTTCATTTACTGTAAGGGGCCCCACAAAT6070.16093752071374595No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5980.15855129717762784No Hit
GTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC5870.15563480174459454No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG5840.15483939389922183No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG5770.1529834422600188No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC5750.15245317036310368No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACG5740.15218803441464612No Hit
CTCCAAAAGAAACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA5490.14555963570320682No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAA5470.14502936380629167No Hit
GTCTTTGAAGTAAGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA5470.14502936380629167No Hit
GTTTGATTTTTTGTACAATATGTTCCTGTGGCAATGTGCCCCAACTCCCA5420.1437036840640038No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5380.14264314027017355No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5170.13707528535256452No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACC5100.13521933371336153No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTC5090.13495419776490394No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG5080.13468906181644638No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGTGGGTCACCATATT5050.13389365397107367No Hit
CCTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCT5000.1325679742287858No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACACCCGTG4970.1317725663834131No Hit
ATTTGAAGGAGGACATTGTTGATAGATGTAAGCAATTTGTGGGGCCCCTT4850.12859093500192223No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAACAG4800.12726525525963436No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT4800.12726525525963436No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4760.1262047114658041No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGAAA4720.1251441676719738No Hit
ATTTACAGGAGGACATTGTTGATAGATGTAAGCAATTTGTGGGGCCCCTT4660.12355335198122837No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG4620.12249280818739808No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA4620.12249280818739808No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT4600.12196253629048294No Hit
GAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACA4560.12090199249665265No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4540.12037172059973751No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT4440.11772036111516179No Hit
GCTATTGTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAG4410.11692495326978908No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA4400.1166598173213315No Hit
GTCCAGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC4390.11639468137287394No Hit
CCTTCAAATGAATGTCAGGAAAAGAAGGAGTGCGCCAGTTAGGATTAAAG4280.11347818593984065No Hit
GGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGA4240.11241764214601037No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC4240.11241764214601037No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4230.11215250619755279No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA4200.11135709835218008No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCTAAACAGGCTTTTACTTT4180.11082682645526494No Hit
CATTTAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT4160.11029655455834979No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC4120.10923601076451951No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGTCTGGA4110.10897087481606194No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA4080.10817546697068922No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG4050.10738005912531651No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG4010.10631951533148622No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG4010.10631951533148622No Hit
CCCTTTTTTCAAAGCCAACTCAGAAAATCCAGATTGGGACCTCAACCCGC3990.10578924343457108No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT3990.10578924343457108No Hit
GTACAATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCC3960.10499383558919836No Hit
GTCCTGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGCAGTATGGATCGG3960.10499383558919836No Hit
CCTGTAAATGAATGTCAGGAAAAGAAGGAGTGCGCCAGTTAGGATTAAAG3910.1036681558469105No Hit
GAGCACGTAGTTAATCATTACTTCCAGACGAGACATTATTTACACACTCT3900.10340301989845294No Hit
GTTAAGGGAATATCCCCATCTTTTTGTTTTGTGCGGGTTTAAATGTATAC3850.10207734015616507No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT3840.10181220420770751No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA3790.10048652446541964No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT3790.10048652446541964No Hit
GTCTTTGGGTATACATTTAAACCCGCACAAAACAAAAAGATGGGGATATT3780.10022138851696208No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)