FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489821

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489821
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences346323
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA24050.6944384288655389No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG22740.6566124687069586No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT21670.6257164554476602No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA18200.5255209731955429No Hit
GGGACTTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTGAAAATCAGA18040.5209010085960216No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT16180.467193920126587No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAACCGTAT14900.430234203330417No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG13850.3999156856460588No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA13400.3869220352099052No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCTCCCACG12850.3710409068990509No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA12370.3571810131004871No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11670.3369686679775816No Hit
CTATCAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG11290.3259962520537186No Hit
ACCTTGAGGAGTGCGAATCCACACTCCAAAAGATACCAAATACTCAAGAA10090.2913465175573092No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA9960.2875927963201982No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG9790.2826840839332068No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT9430.27228916358428407No Hit
GTACATTCTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA9180.26507046889753205No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT8720.25178807067390846No Hit
GGATAATACGGTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTAAGGTT8660.250055583949088No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8540.24659061049944705No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGCTCACCTCACCATACAG8320.24023815917510535No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCATCTGCGGA7850.22666701316401164No Hit
GTTCAGGATAATACGGTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTA7720.2229132919269006No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAAC7320.2113633804280975No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC7300.2107858848531573No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7220.20847590255339668No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA7090.20472218131628567No Hit
GTGTAAAAGGGGCAGCAAAGCCCAGAAGGCCCACAATTCTTTGACATACT6860.19808098220447387No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6750.194904756542303No Hit
GTATACATGCATATAACGGCATCAAGGCAGGATAGCCACATTGTGTAAAA6650.1920172786676022No Hit
GCCTTAGAGTCTCCGGAACATTGCTCACCTCACCATACAGCACTCAGGCA6340.18306609725602976No Hit
CAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTGTGGTG6160.17786863708156836No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6080.17555865478180774No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTGTTCG6010.17353742026951718No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT6000.17324867248204712No Hit
TTATTGATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA5870.16949495124493608No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG5810.16776246452011562No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT5790.16718496894517546No Hit
CTTCTGGACTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC5720.1651637344328849No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5520.15938877868348333No Hit
ACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCCAGGTGGC5450.1573675441711928No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG5350.154480066296492No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5220.15072634505938098No Hit
GTCCACAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA5150.14870511054709043No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG5120.1478388671846802No Hit
CTACTGTACCTGTCTTTAATCCCGAGTGGCAAACTCCCTCCTTTCCTCAC4970.14350765037262903No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA4850.14004267692298808No Hit
CATTTAAACCCTAATAAAACCAAGCGTTGGGGCTACTCCCTTAACTTCAT4820.13917643356057785No Hit
GGGTAAAGGTTCAGATATTGTTTACACAAAAAGGCCTTGTAAGTTGGCGA4800.1385989379856377No Hit
CCTCAAGGTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4730.13657770347334713No Hit
ACTCACCCCAACACAGAATAGCTTGCCTGAGTGCTGTATGGTGAGGTGAG4720.13628895568587707No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCATCTGCGG4650.13426772117358649No Hit
GTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAAC4630.13369022559864635No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAAT4560.1316689910863558No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCGTTATATGCATGTATA4540.13109149551141563No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA4520.1305139999364755No Hit
GTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT4520.1305139999364755No Hit
GTCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATAA4510.1302252521490054No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC4510.1302252521490054No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA4490.12964775657406524No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC4490.12964775657406524No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATACGGTTTAATGCC4450.12849276542418495No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAA4270.12329530524972353No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4250.12271780967478337No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACATAAATC4180.12069657516249283No Hit
ATGTTAATATGGGCCTGAAAATCAGACAACTATTGTGGTTTCACATTTCC4140.1195415840126125No Hit
GTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGTACAATA4140.1195415840126125No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACGAGAAAAACCCC4090.11809784507526211No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT4000.1154991149880314No Hit
ACCTTGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA4000.1154991149880314No Hit
CTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCCAGGTGGCC3990.11521036720056133No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA3980.11492161941309126No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA3970.11463287162562118No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTG3960.1143441238381511No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC3910.1129003849008007No Hit
GTCCAAGGGATACTAACATTGAGAGTCCCGAGATTGAGATCTTCTGCGAC3860.1114566459634503No Hit
AACCTTGAGGAGTGCGAATCCACACTCCAAAAGATACCAAATACTCAAGA3850.11116789817598022No Hit
AGCGGGAGGAGTGCGAATCCACACTCCAAAAGATACCAAATACTCAAGAA3840.11087915038851014No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT3750.10828042030127945No Hit
CTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCT3690.10654793357645898No Hit
TGGAAACCACCGTGAACGCCCACCAGGTCTTGCCCAAGGTCTTACATAAG3680.1062591857889889No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACACAGTCTTTGA3680.1062591857889889No Hit
GATTAACTGCATGTTCAGGATAATACGGTTTAATGCCTTTGTCTAAGGGC3670.10597043800151883No Hit
ATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGAT3650.10539294242657865No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC3630.10481544685163849No Hit
AGTTAAGGGAGTAGCCCCAACGCTTGGTTTTATTAGGGTTTAAATGTATA3620.10452669906416841No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA3610.10423795127669834No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC3610.10423795127669834No Hit
TTCTTCTACTGTACCTGTCTTTAATCCCGAGTGGCAAACTCCCTCCTTTC3600.10394920348922826No Hit
CCTCTGCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGCTCACCT3590.10366045570175818No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT3580.1033717079142881No Hit
GGGAATTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTGAAAATCAGA3570.10308296012681803No Hit
GTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCC3560.10279421233934795No Hit
GGGCACATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATC3550.10250546455187787No Hit
GTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTT3550.10250546455187787No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT3550.10250546455187787No Hit
GCCCAGAAGGCCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT3530.10192796897693772No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT3520.10163922118946764No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC3500.10106172561452748No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG3490.10077297782705741No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)