FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489823

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489823
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences399265
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA18900.4733698170388088No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTACATGTTCAGG12410.3108211338334189No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG12230.3063128498616207No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11750.2942907592701589No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCATAT11560.2895320150777053No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATCATGCCTGCTAGG11540.28903109463639437No Hit
GGTTAGGATAGAACCTAGCAGGCATGATTAATTTTAATCTCCTTTTTTCA11200.2805154471341089No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT10870.2722502598524789No Hit
GTACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG10390.2602281692610171No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9750.24419871513906805No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC9750.24419871513906805No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA9080.22741788035515262No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA8930.22366097704532079No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8720.21840131241155622No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA8570.21464440910172441No Hit
GCCTTAGAGTCTCCGGAACATTGCTCACCTCACCATACAGCACTCAGGCA8380.2098856649092708No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGCATA8000.2003681765243635No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT7830.19611035277322078No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT7830.19611035277322078No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA7700.1928543699046999No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA7690.19260390968404442No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCATGT7660.19185252902207808No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT7500.1878451654915908No Hit
GGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT7480.18734424505027988No Hit
CTCCAAAAGACACCAAATATTCTAGAACAGTTTCTCTTCCAAAAGTAAGA7250.18158365997520443No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC7190.18008089865127172No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC6610.16555420585325537No Hit
GTATAAGCAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC6600.16530374563259992No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6570.16455236497063355No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG6400.16029454121949083No Hit
GTCCCGTACTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6290.1575394787922808No Hit
GTCTTACATAAGAGGACTCTTGGACTTTTACATAAGAGGACTCTTGGACT6190.1550348765857263No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCTTCATTTCCTCAC6060.15177889371720538No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGT6020.15077705283458354No Hit
GGGTAAAGGTTCAGATATTGCTTATACAGAAAGGCCTTGTAAGTTGGCGA5950.14902383128999536No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA5870.14702014952475173No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC5870.14702014952475173No Hit
CTATTAATAATGTCCTCATGTAAATGAATGTGAGGAAATGAAGGAGTTTG5850.1465192290834408No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGACCCGTAT5700.142762325773609No Hit
CTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCAGTGGA5600.14025772356705446No Hit
CTCTAAGGCCTCCCGATACAAAGCTGAGGCGGTGTCGAGGAGATCTCGAA5510.13800358158115536No Hit
GGATAGAACCTAGCAGGCATGATTAATTTTAATCTCCTTTTTTCATTAAC5490.13750266113984447No Hit
GTCTTACATAAGAGGACTCTTGGACTTTCAGCAATGTCAACGACCGACCT5450.13650082025722263No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5410.13549897937460084No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCAGTGGAGTTACTCTCTTTT5400.1352485191539454No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5360.13424667827132356No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5360.13424667827132356No Hit
CTTTAATCCTGAGTGGCAAACTCCTTCATTTCCTCACATTCATTTACATG5350.1339962180506681No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC5320.13324483738870174No Hit
ATATAGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5310.1329943771680463No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5290.13249345672673538No Hit
CTCATCTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGG5170.1294879340788699No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG5160.12923747385821446No Hit
GTCTACGTCCCGTCGACGCTGAATCCCGCGGACGACCCCTCTCGGGGCCG5130.1284860931962481No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCTTACAGACCACCAAATGCC5070.12698333187231536No Hit
GTTTGGGGCTCTACCGTCCCCTTCTTCATCTGCCGTTCCGGCCGACCACG5050.12648241143100447No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCAGTGG5030.12598149098969355No Hit
GTTCAGGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTA4990.12497965010707175No Hit
GATCCATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGCAGGTCTG4950.12397780922444994No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4930.12347688878313902No Hit
GCTTAGATTGTATGCATGCATATAAAGGCATTAAGGCAGGATAGCCACAT4890.1224750479005172No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTAATCTCCTC4820.12072182635592901No Hit
GTTGTCCTCTCTCGGAAATACACCTCCTTTCCATGGCTGCTAGGGTGTGC4800.1202209059146181No Hit
GTGTTGGGGTGAGTTGATGAATTTGGCCACCTGGGTGGGAAGTAATTTGG4750.11896860481134083No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG4740.11871814459068539No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG4710.11796676392871902No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATTTG4690.1174658434874081No Hit
TTCTAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGGAACCACA4670.1169649230460972No Hit
AGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCTAGAA4640.11621354238413083No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC4630.11596308216347538No Hit
TCCTTTGTCTACGTCCCGTCGACGCTGAATCCCGCGGACGACCCCTCTCG4600.11521170150150903No Hit
TGCTTATACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT4590.11496124128085358No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4570.11446032083954266No Hit
CCTAGTACAAAGATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTC4550.11395940039823176No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTAGTACAATA4550.11395940039823176No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC4540.11370894017757631No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC4520.11320801973626539No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG4490.11245663907429902No Hit
GATTGTATGCATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGT4490.11245663907429902No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC4490.11245663907429902No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4480.11220617885364358No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGGCGATGGCC4450.11145479819167721No Hit
TGGAAACCACCGTGAACGCCCACCAGGTCTTGCCCAAGGTCTTACATAAG4370.10945111642643357No Hit
GTATAAAGAATTTGGAGCTTCAGTGGAGTTACTCTCTTTTTTGCCTTCTG4330.10844927554381174No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC4320.1081988153231563No Hit
CATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA4280.10719697444053447No Hit
ATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGCATACAATCTAAGC4240.10619513355791266No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4230.1059446733372572No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA4180.10469237223397995No Hit
ATATTATCCTGAACATGTAGTTAATCATTACTTCAAAACTAGGCATTATT4180.10469237223397995No Hit
CATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTC4170.10444191201332449No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTACATGTTCA4170.10444191201332449No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTACTGTGGTTCCACATTTCC4150.10394099157201359No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4140.10369053135135813No Hit
CTCTTGGACTTTCAGCAATGTCAACGACCGACCTTGAGGCATACTTCAAA4110.10293915068939176No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA4030.10093546892414812No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTACA4000.10018408826218175No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)