FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200158
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC6950.3472256917035542No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACACAGTCTTTGA6930.34622648107994686No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT6610.33023911110222925No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA6090.3042596348884381No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG5750.2872730542871132No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5660.2827766064808801No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT5330.26628963119135884No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5240.26179318338512575No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG4980.24880344527823023No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA4890.24430699747199713No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTAT4790.23931094435396036No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA3930.19634488753884433No Hit
ATATAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC3860.1928476503562186No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG3730.1863527813027708No Hit
ATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGCACTTG3590.17935830693751936No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCAACAATTCTTTGACATACT3460.1728634378840716No Hit
CCCTTAACTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG3310.16536935820701645No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCGTAT3270.16337093695980176No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT3230.16137251571258707No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT3220.1608729104007834No Hit
GGGTAAAGGTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA3100.15487764665913928No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTC3090.1543780413473356No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA3090.1543780413473356No Hit
CTTATATAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCA2980.14888238291749517No Hit
CTATATAAGAGAGAAACTACACGTAGCGCCTCATTTTGTGGGTCACCATA2960.14788317229388784No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA2950.14738356698208416No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA2850.1423875138640474No Hit
CGTTAATATGGGCCTAAAACTCAGACAAATATTGTGGTTTCACATTTCCT2810.1403890926168327No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG2800.139889487305029No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA2780.13889027668142168No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2690.13439382887518858No Hit
TCTGTGCAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTATTCG2680.13389422356338493No Hit
ACATCGACCCATATAAAGAATTTGGAGCATCTGTGGAGTTACTCTCTTTT2670.13339461825158125No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAATTAATGAAAAA2590.12939777575715186No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT2500.12490132795091877No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG2500.12490132795091877No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA2460.12290290670370409No Hit
TCACCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA2420.12090448545648937No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG2420.12090448545648937No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA2410.12040488014468569No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT2380.11890606420927466No Hit
TAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAACATACCTTGGTAG2370.118406458897471No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT2360.11790685358566731No Hit
CTTTTGGAGTGTGGATTCGCACTCCTACCGCTTACAGACCACCAAATGCC2350.11740724827386366No Hit
CTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCTTG2330.11640803765025629No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA2300.11490922171484526No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG2280.11391001109123793No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG2280.11391001109123793No Hit
GTCCCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCTTCCCGACTGCCGAT2270.11341040577943423No Hit
GGTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG2250.1124111951558269No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTATTCG2220.11091237922041587No Hit
GAGTGATTCATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTCTTGCATGG2210.1104127739086122No Hit
GTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA2200.10991316859680852No Hit
CTTGATTTTGAGTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAA2200.10991316859680852No Hit
TTTCTGTTCCCAATCCTCTGGGATTCTTTCCCGATCACCAGTTGGACCCT2200.10991316859680852No Hit
GCCTATAGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC2180.10891395797320118No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCAACAATTCT2170.1084143526613975No Hit
CATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA2130.10641593141418279No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG2120.10591632610237912No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT2100.10491711547877178No Hit
ATATAAGAGAGAAACTACACGTAGCGCCTCATTTTGTGGGTCACCATATT2090.1044175101669681No Hit
GTCAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTTGGTCTTTT2070.10341829954336075No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC2070.10341829954336075No Hit
GATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGT2040.10191948360794972No Hit
TTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT2020.10092027298434236No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA2020.10092027298434236No Hit
CATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAG2010.1004206676725387No Hit
CATATACACCATGGAATACTATGCAGCCATAAAAAACGATGAGTTCATGT2010.1004206676725387No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG2010.1004206676725387No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCGATTCG2010.1004206676725387No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)