FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200158
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC6020.3007623977058124No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA5870.2932683180287573No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT5770.28827226491072055No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5580.2787797639864507No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG5450.27228489493300295No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5430.2712856843093956No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG4760.23781212841854932No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA4740.236812917794942No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCAACAATTCTTTGACATACT4610.23031804874149422No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG4450.22232436375263542No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTAT4380.2188271265700097No Hit
ATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGCACTTG4050.20234015128048843No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC3910.19534567691523697No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACACAGTCTTTGA3780.18885080786178918No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA3710.18535357067916347No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT3660.1828555441201451No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT3550.17735988569030464No Hit
CCCTTAACTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG3530.1763606750666973No Hit
GGGTAAAGGTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA3310.16536935820701645No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG3260.16287133164799808No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCGTAT3110.15537725197094296No Hit
CTTTTGGAGTGTGGATTCGCACTCCTACCGCTTACAGACCACCAAATGCC3110.15537725197094296No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA3100.15487764665913928No Hit
GTCCCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCTTCCCGACTGCCGAT2890.1443859351112621No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTC2890.1443859351112621No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT2880.14388632979945845No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG2730.1363922501224033No Hit
ATATAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC2680.13389422356338493No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2680.13389422356338493No Hit
CTATATAAGAGAGAAACTACACGTAGCGCCTCATTTTGTGGGTCACCATA2630.13139619700436656No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAATTAATGAAAAA2610.1303969863807592No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA2600.12989738106895554No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA2580.12889817044534818No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC2500.12490132795091877No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG2500.12490132795091877No Hit
GTCAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTTGGTCTTTT2490.12440172263911511No Hit
ACATCGACCCATATAAAGAATTTGGAGCATCTGTGGAGTTACTCTCTTTT2480.12390211732731142No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA2440.12190369608009671No Hit
GGTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG2410.12040488014468569No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA2350.11740724827386366No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG2350.11740724827386366No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA2350.11740724827386366No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG2310.11540882702664895No Hit
ATATGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTG2280.11391001109123793No Hit
CTCTTACAATTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG2280.11391001109123793No Hit
TCTGTGCAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTATTCG2220.11091237922041587No Hit
GTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA2180.10891395797320118No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA2180.10891395797320118No Hit
GAGTGATTCATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTCTTGCATGG2170.1084143526613975No Hit
GTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT2090.1044175101669681No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT2050.10241908891975339No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG2040.10191948360794972No Hit
ATATAAGAGAGAAACTACACGTAGCGCCTCATTTTGTGGGTCACCATATT2040.10191948360794972No Hit
CTTATATAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCA2030.10141987829614604No Hit
CTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCTTG2030.10141987829614604No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)