FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences288662
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC19750.6841911993958333No Hit
CTTTTATATAAGATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTCGT17630.6107489035619513No Hit
TCCACAGAGTGTGTAAATAATGTCTCGTTTGGAAGTAATGATTAACTACA17490.6058989406295252No Hit
GGGTCTACATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTA14230.4929640894887446No Hit
GTGTGTAAATAATGTCTCGTTTGGAAGTAATGATTAACTACATGCTCTGG13400.46421073781793243No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA12850.44515731201197245No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG12730.44100020092703573No Hit
GTGTAAATAATGTCTCGTTTGGAAGTAATGATTAACTACATGCTCTGGAT11950.41397897887494717No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT11210.38834346051783747No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10790.373793571720559No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA8810.30520123881910327No Hit
CTATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGT8580.2972334425729746No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG8360.28961207225059066No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT8330.28857279447935646No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA8270.28649423893688813No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT8090.2802585723094831No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACACCCAATTACATATCCCATGAA7780.26951936867339654No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA7750.2684800909021624No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT7710.2670943872071835No Hit
TGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAAGGGACTC7360.25496947987611807No Hit
ATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTTT7240.2508123687911814No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG7060.24457670216377633No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6770.23453035037517928No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGGAAAACCCC6610.22898753559526366No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA6540.22656255412905058No Hit
ATATAAGATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTCGTTTGGA6470.2241375726628375No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAAAAAGGCCTTGTAAGTTGGCGA6460.22379114673909276No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGTTCCACCCCAACAGTCCCCCATG6380.221019739349135No Hit
CTCTTTTATATAAGATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTC6320.2189411838066666No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC6280.21755548011168774No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC6140.21270551717926153No Hit
GCCTAGCAGCCATGGGAACGATGTATACTTGCGGGAGAGCACGACAGAAT6080.2106269616367932No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG5940.20577699870436705No Hit
GACCAGACCGTTGTCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT5870.20335201723815396No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5860.20300559131440923No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA5770.1998877580007067No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT5760.19954133207696198No Hit
CGTGTGGACTCTCTTTTATATAAGATACCCGCCTTCCACAGAGTGTGTAA5740.19884848022947255No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACGG5630.19503779506828056No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT5550.19226638767832274No Hit
CAATAGGTCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT5460.18914855436462022No Hit
GTTCAGATACTGTTTACTTAAAAAGGCCTTGTAAGTTGGCGAGAAAGTAA5370.18603072105091767No Hit
CTCATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5320.18429859143219404No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG5160.17875577665227843No Hit
AGTATACATCGTTCCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG5140.178062924804789No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA5130.17771649888104427No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG5090.17633079518606537No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC5080.17598436926232064No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC5070.1756379433385759No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA5020.17390581371985228No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5000.17321296187236282No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAAACGAGACATTATT4890.16940227671117083No Hit
ATGTAATTGGGTGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA4860.16836299893993667No Hit
GCACAACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACA4840.1676701470924472No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC4780.16559159154997885No Hit
ACACACAATAGCTTGCCTGAGTGCCGTATGGTGAGGTGAACAATGTTCCG4730.16385946193125522No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGTCTGT4710.1631666100837658No Hit
TAGATAAAGGGATCAAACCTTATTATCCAGAGCATGTAGTTAATCATTAC4670.1617809063887869No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC4640.1607416286175527No Hit
ATTGTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA4550.15762379530385018No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTA4540.15727736938010545No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4420.15312025829516876No Hit
CACATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACGGTACCTTGC4410.152773832371424No Hit
GCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA4410.152773832371424No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT4360.1510417027527004No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT4340.15034885090521094No Hit
GGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGA4320.14965599905772148No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4250.14723101759150842No Hit
TTGTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAAC4250.14723101759150842No Hit
ATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTGTG4220.14619173982027422No Hit
TCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCACT4220.14619173982027422No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG4220.14619173982027422No Hit
ACTACATGCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATA4200.1454988879727848No Hit
ACCTTGCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCTGACATTCATT4200.1454988879727848No Hit
GTCCGGTAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4160.14411318427780587No Hit
CATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT4110.14238105465908224No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4100.14203462873533754No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC4080.14134177688784808No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGC4060.1406489250403586No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG4050.14030249911661388No Hit
GGTTCAGATACTGTTTACTTAAAAAGGCCTTGTAAGTTGGCGAGAAAGTA4030.13960964726912445No Hit
CACACAATAGCTTGCCTGAGTGCCGTATGGTGAGGTGAACAATGTTCCGG4010.138916795421635No Hit
ATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAACCTTAT4000.13857036949789026No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG3990.13822394357414555No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA3950.13683823987916663No Hit
CCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGGT3910.13545253618418773No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTCTACATTTAA3820.1323347028704852No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC3810.13198827694674048No Hit
CTTTATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGC3800.13164185102299575No Hit
CATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACGGTACCTTGCTT3790.13129542509925102No Hit
CTACCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC3790.13129542509925102No Hit
TTGACATACTTTCCAATCAATAGGTCTGTTTACAGGAAGTTTCCTAAAAC3780.13094899917550631No Hit
CAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTAT3770.13060257325176158No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG3770.13060257325176158No Hit
GTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACTGGGCTTTATTC3720.12887044363303796No Hit
GCCTTCCACAGAGTGTGTAAATAATGTCTCGTTTGGAAGTAATGATTAAC3680.12748473993805903No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA3680.12748473993805903No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT3680.12748473993805903No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT3680.12748473993805903No Hit
TGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGA3630.1257526103193354No Hit
GATTAACTACATGCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGC3630.1257526103193354No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG3620.1254061843955907No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC3560.12332762885312235No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA3530.12228835108188817No Hit
GAGTGTGTAAATAATGTCTCGTTTGGAAGTAATGATTAACTACATGCTCT3530.12228835108188817No Hit
GTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATAT3500.12124907331065397No Hit
CTCTGTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC3470.12020979553941981No Hit
GCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAG3470.12020979553941981No Hit
TCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATT3390.11743838814946199No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT3380.11709196222571727No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT3280.11362770298827002No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGTGAGG3280.11362770298827002No Hit
CTTCAAAGACTGTGTGTTTAATGAGTGGGAGGAGTTGGGGGAGGAGATTA3250.11258842521703584No Hit
CTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCTGAC3240.11224199929329112No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGT3230.11189557336954638No Hit
GTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGCAAGACCTTGGG3190.1105098696745675No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG3170.10981701782707803No Hit
GCTTTGGGGCATGGACATTGACACGTATAAAGAATTTGGAGCTTCTGTGG3140.10877774005584387No Hit
GGTCTGGTCTGTGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGC3140.10877774005584387No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGTACAA3120.10808488820835442No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC3120.10808488820835442No Hit
CCTGTAAACAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT3090.10704561043712023No Hit
CACTTGGCACAGACCAGACCGTTGTCGAGCAACGGGGTAAAGGTTCAGAT3080.10669918451337551No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG3080.10669918451337551No Hit
CCCTTAACTTCATGGGATATGTAATTGGGTGTTGGGGCACATTGCCACAG3080.10669918451337551No Hit
CTGTTGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCCC3060.10600633266588605No Hit
GAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACA3060.10600633266588605No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA3050.10565990674214133No Hit
CTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCT3040.1053134808183966No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT3030.10496705489465188No Hit
CTCTTATGCAAGACCTTGGGCAGGTTCCGGTGAGCGTTCACGGTGGTCTC3020.10462062897090714No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGTAGATGAGAAGGCACAG3020.10462062897090714No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA2970.10288849935218353No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC2960.10254207342843881No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA2960.10254207342843881No Hit
GTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCCGC2920.1011563697334599No Hit
GAGTCGTGCAGGTTTTGCATGGTCCGGTGCTGGTTGTTGATGATCCTGGA2920.1011563697334599No Hit
CCTTCCACAGAGTGTGTAAATAATGTCTCGTTTGGAAGTAATGATTAACT2910.10080994380971516No Hit
TCGCAGAAGATCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACA2900.10046351788597044No Hit
GGTCTACATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAA2890.10011709196222572No Hit
CTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTG2890.10011709196222572No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)