FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences288662
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATAAGATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTCGT17260.5979311443833966No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC17110.5927347555272257No Hit
TCCACAGAGTGTGTAAATAATGTCTCGTTTGGAAGTAATGATTAACTACA16470.5705634964075632No Hit
GTGTGTAAATAATGTCTCGTTTGGAAGTAATGATTAACTACATGCTCTGG15140.5244888485495146No Hit
GGGTCTACATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTA13480.4669821452078902No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG12830.44446446016448304No Hit
GTGTAAATAATGTCTCGTTTGGAAGTAATGATTAACTACATGCTCTGGAT12510.43337883060465177No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT11980.41501825664618136No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA11340.3928469975265189No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9700.3360331460323839No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT8760.30346910920037967No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG8330.28857279447935646No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA8280.2868406648606328No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT8030.2781800167670147No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA7690.266401535359694No Hit
CTATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGT7070.24492312808752104No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT7020.24319099846879744No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACACCCAATTACATATCCCATGAA6920.23972673923135018No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA6800.23556962814641347No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGTTCCACCCCAACAGTCCCCCATG6670.23106609113773205No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG6590.22829468374777423No Hit
TGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAAGGGACTC6550.2269089800527953No Hit
AGTATACATCGTTCCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG6520.22586970228156114No Hit
ATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTTT6460.22379114673909276No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAAAAAGGCCTTGTAAGTTGGCGA6400.22171259119662443No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6330.21928760973041134No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC6270.217209054187943No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC6270.217209054187943No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA6190.2144376467979852No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGGAAAACCCC6180.21409122087424046No Hit
CTCTTTTATATAAGATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTC6140.21270551717926153No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC5890.2040448690856434No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT5780.20023418392445144No Hit
ATATAAGATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTCGTTTGGA5710.19780920245823838No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG5650.19573064691577No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT5540.19191996175457804No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACGG5500.19053425805959912No Hit
GTTCAGATACTGTTTACTTAAAAAGGCCTTGTAAGTTGGCGAGAAAGTAA5470.18949498028836495No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA5430.18810927659338603No Hit
GCCTAGCAGCCATGGGAACGATGTATACTTGCGGGAGAGCACGACAGAAT5320.18429859143219404No Hit
GACCAGACCGTTGTCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT5270.18256646181347042No Hit
CAATAGGTCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT5240.18152718404223625No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5190.17979505442351262No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC5170.17910220257602316No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA5140.178062924804789No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG5140.178062924804789No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAAACGAGACATTATT4970.17217368410112865No Hit
ATGTAATTGGGTGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA4960.17182725817738392No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA4930.17078798040614976No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG4860.16836299893993667No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC4780.16559159154997885No Hit
CTCATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA4640.1607416286175527No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT4640.1607416286175527No Hit
CGTGTGGACTCTCTTTTATATAAGATACCCGCCTTCCACAGAGTGTGTAA4630.160395202693808No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGTCTGT4630.160395202693808No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC4620.16004877677006324No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTA4600.1593559249225738No Hit
ACTACATGCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATA4450.15415953606640292No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC4440.1538131101426582No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4390.15208098052393457No Hit
GGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGA4370.1513881286764451No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG4350.15069527682895567No Hit
GCACAACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACA4320.14965599905772148No Hit
ATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTGTG4310.14930957313397678No Hit
GCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA4270.14792386943899785No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4200.1454988879727848No Hit
CTACCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC4140.1434203324303164No Hit
CTTCAAAGACTGTGTGTTTAATGAGTGGGAGGAGTTGGGGGAGGAGATTA4130.1430739065065717No Hit
CCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGGT3990.13822394357414555No Hit
TAGATAAAGGGATCAAACCTTATTATCCAGAGCATGTAGTTAATCATTAC3960.13718466580291136No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT3950.13683823987916663No Hit
CACATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACGGTACCTTGC3910.13545253618418773No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC3900.135106110260443No Hit
ATTGTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA3890.13475968433669827No Hit
GCCTTCCACAGAGTGTGTAAATAATGTCTCGTTTGGAAGTAATGATTAAC3880.13441325841295357No Hit
ACCTTGCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCTGACATTCATT3880.13441325841295357No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTCTACATTTAA3850.1333739806417194No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA3840.13302755471797464No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGC3820.1323347028704852No Hit
GGTTCAGATACTGTTTACTTAAAAAGGCCTTGTAAGTTGGCGAGAAAGTA3810.13198827694674048No Hit
CTTTATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGC3800.13164185102299575No Hit
ATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAACCTTAT3800.13164185102299575No Hit
TTGACATACTTTCCAATCAATAGGTCTGTTTACAGGAAGTTTCCTAAAAC3740.1295632954805274No Hit
GGTCTGGTCTGTGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGC3730.12921686955678266No Hit
GTCCGGTAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC3710.12852401770929323No Hit
GATTAACTACATGCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGC3710.12852401770929323No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG3710.12852401770929323No Hit
TCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATT3680.12748473993805903No Hit
CCTGTAAACAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT3650.12644546216682487No Hit
GAGTGTGTAAATAATGTCTCGTTTGGAAGTAATGATTAACTACATGCTCT3650.12644546216682487No Hit
GTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATAT3540.12263477700563288No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT3520.12194192515814344No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC3490.12090264738690926No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG3480.12055622146316453No Hit
TTGTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAAC3440.11917051776818563No Hit
CACACAATAGCTTGCCTGAGTGCCGTATGGTGAGGTGAACAATGTTCCGG3430.1188240918444409No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGT3430.1188240918444409No Hit
ACACACAATAGCTTGCCTGAGTGCCGTATGGTGAGGTGAACAATGTTCCG3430.1188240918444409No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG3400.11778481407320673No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG3370.11674553630197256No Hit
CAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTAT3350.11605268445448311No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG3330.11535983260699365No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG3320.11501340668324891No Hit
TCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCACT3320.11501340668324891No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA3290.11397412891201474No Hit
ACCTTATTATCCAGAGCATGTAGTTAATCATTACTTCCAAACGAGACATT3290.11397412891201474No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT3270.11328127706452529No Hit
CTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCT3270.11328127706452529No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA3240.11224199929329112No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGTGAGG3240.11224199929329112No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC3230.11189557336954638No Hit
CTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTG3230.11189557336954638No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC3180.11016344375082275No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA3150.10912416597958859No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG3140.10877774005584387No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC3130.10843131413209914No Hit
CATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACGGTACCTTGCTT3100.10739203636086496No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA3100.10739203636086496No Hit
ATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGCATAA3090.10704561043712023No Hit
TGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGA3060.10600633266588605No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT3060.10600633266588605No Hit
CTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCTGAC3050.10565990674214133No Hit
CATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT3030.10496705489465188No Hit
CTCTGTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC3030.10496705489465188No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG3000.10392777712341769No Hit
GCCCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTA3000.10392777712341769No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT2990.10358135119967297No Hit
CCTTTACCCCGTTGCTCGACAACGGTCTGGTCTGTGCCAAGTGTTTGCTG2970.10288849935218353No Hit
GAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACA2960.10254207342843881No Hit
CTGTTGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCCC2960.10254207342843881No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT2940.10184922158094935No Hit
CCCTTAACTTCATGGGATATGTAATTGGGTGTTGGGGCACATTGCCACAG2930.10150279565720462No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT2910.10080994380971516No Hit
GCTTTGGGGCATGGACATTGACACGTATAAAGAATTTGGAGCTTCTGTGG2900.10046351788597044No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)