FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489828

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489828
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326984
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTACATGGTCCGG20610.6303060700217747No Hit
CTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT18950.579539060015169No Hit
TAGACAAAGGCATTAAACCTTATTATCCGGACCATGTAGTTAATCATTAC18470.5648594426638612No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG16570.5067526239816015No Hit
GTGTTGAAGACCAACCTCCCATGCTGTAGCTCTTGTTCCCAAGAATATGG13990.42784968071832263No Hit
CTCTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT13050.3991020967386783No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC11990.36668460842120715No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11230.3434418809483033No Hit
CTTTATCTAAGGGCAAATATTTGGTAAGGTTAGGATAAAACCTAGCAGGC10710.32753896215105327No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT10660.3260098353436254No Hit
CATACAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA10520.3217282802828273No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10470.3201991534753994No Hit
ATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATG9490.29022826804981283No Hit
ACAATATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAA9400.28747583979644264No Hit
ATTTTGTGGTCTGTAAGCGGGAGGAGTGCGAATCCACACTCCAAAAGACA9290.28411176082010126No Hit
CTTCCACACCTCGAAAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCT8920.27279622244513496No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8310.2541408753945147No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTACA8270.2529175739485724No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC8000.24466028918846183No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTACATGGTCC7980.24404863846549069No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT7710.2357913537053801No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7490.2290631957526974No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA7470.2284515450297262No Hit
GAAATTTCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGCTTGTATA7470.2284515450297262No Hit
GTCCCGTACTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6790.20765542044870697No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA6730.2058204682797935No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAA6330.19358745382037043No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT6280.19205832701294254No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6230.19052920020551464No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC6050.18502434369877424No Hit
GTACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG6030.1844126929758031No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAACCTTAT6020.18410686761431752No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC6010.18380104225283195No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTAT6010.18380104225283195No Hit
CTCCAAAAGACACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGA5840.17860201110757715No Hit
CTTTATATGCATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACT5780.17676705893866365No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATGTCCTCCTGC5740.17554375749272136No Hit
GTACAAAAACTCAAGCAATGTTTTCGCAAACTTCCTGTAAATAGACCTAT5700.17432045604677904No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTGCAGG5660.17309715460083674No Hit
CCCTTAACTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG5660.17309715460083674No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5550.16973307562449538No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAAGTTAAGGGAG5470.16728647273261077No Hit
ACCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT5460.1669806473711252No Hit
GAAAAGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGCTTGTATA5300.16208744158735597No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAAGCCTTGTAAGTTGGCGA5300.16208744158735597No Hit
TATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATGAAAAAA5280.1614757908643848No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA5280.1614757908643848No Hit
GTGTAGTTTCTCTCTTATATAGAATACCAGCCTTCCACAGAGTATGTAAA5240.1602524894184425No Hit
ACTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCACCACCAATCGGCAGT5180.15841753724952903No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5170.15811171188804346No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5000.15291268074278863No Hit
GCATTAAACCTTATTATCCGGACCATGTAGTTAATCATTACTTCAAAACT4970.1519952046583319No Hit
CAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATGAAAAAAGGA4960.15168937929684634No Hit
CCTTTATCTAAGGGCAAATATTTGGTAAGGTTAGGATAAAACCTAGCAGG4920.15046607785090402No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4890.14954860176644727No Hit
CTCACCAACCTCTTGTCCTCCAATTTGTCCTGGCTATCGTTGGATGTGTC4860.14863112568199055No Hit
GATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACAC4810.14710199887456266No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT4730.14465539598267804No Hit
ATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTTGA4600.14067966628336553No Hit
ATATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTA4600.14067966628336553No Hit
GCTGAGAGTCCAAGAGTCCGCTTATGTAAGACCTTGGGCAAGACTTGGTG4580.1400680155603944No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4580.1400680155603944No Hit
GCCTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAAC4470.13670393658405305No Hit
GTTTTAGTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA4470.13670393658405305No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA4440.1357864604995963No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT4440.1357864604995963No Hit
GTTGTCAGTATGCCCTGAGTCTGAGGGCTCCACCCCAAAAGACCGCCGTG4420.13517480977662516No Hit
CCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCAA4390.1342573336921684No Hit
GTCTTTGAAGTACGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA4350.13303403224622612No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC4280.13089325471582708No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC4280.13089325471582708No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4260.13028160399285593No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG4250.12997577863137036No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCATATTTCC4250.12997577863137036No Hit
GTGTGGAAGACCAACCTCCCATGCTGTAGCTCTTGTTCCCAAGAATATGG4140.126611699655029No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT4140.126611699655029No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTA4100.12538839820908668No Hit
ATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAG4070.12447092212462996No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG4070.12447092212462996No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG4060.12416509676314437No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4040.12355344604017324No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT4030.12324762067868764No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA4000.12233014459423092No Hit
GTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTAAGGTTAGGATAAAAC4000.12233014459423092No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTACTGGTAGTTGATGTTCCTGG4000.12233014459423092No Hit
GTTTTATGCCTTTGTCTAAGGGCAAATATTTGGTAAGGTTAGGATAAAAC3940.12049519242531745No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC3930.12018936706383186No Hit
ATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATGAAAAAAG3880.11866024025640398No Hit
GCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA3860.11804858953343284No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCT3840.11743693881046167No Hit
GAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAA3840.11743693881046167No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGAAGTTTGCGAAAAC3710.11346120911114918No Hit
ATGTAAGACCTTGGGCAAGACTTGGTGGGCGTTCACGGTGGTCTCCATGC3650.1116262569422357No Hit
GCGTGTAGTTTCTCTCTTATATAGAATACCAGCCTTCCACAGAGTATGTA3620.11070878085777897No Hit
ATTTGGTGGTCTGTAAGCGGGAGGAGTGCGAATCCACACTCCAAAAGACA3590.10979130477332223No Hit
GGACATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATG3590.10979130477332223No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTCTATATAAGAGAGAAACTACA3580.10948547941183667No Hit
CATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA3500.10703887651995206No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC3490.10673305115846646No Hit
GGGTTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGGAAGAT3490.10673305115846646No Hit
GGCCTACATATTGTTGACATCTATTGATAATGTCCTCCTGCAAATGAATG3480.1064272257969809No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA3470.10612140043549532No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG3460.10581557507400974No Hit
GTACTAAAACTCAAGCAATGTTTTCGCAAACTTCCTGTAAATAGACCTAT3440.10520392435103858No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT3430.104898098989553No Hit
CTTCAACACCTCGAAAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCT3420.10459227362806743No Hit
GCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC3380.10336897218212512No Hit
CTTGATTTTTAGTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAA3370.10306314682063955No Hit
GTTTTGAAGTAATGATTAACTACATGGTCCGGATAATAAGGTTTAATGCC3350.10245149609766839No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA3340.10214567073618282No Hit
ACATATTGTTGACATCTATTGATAATGTCCTCCTGCAAATGAATGTGAGG3340.10214567073618282No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC3320.10153402001321166No Hit
GCATAAAACCTTATTATCCGGACCATGTAGTTAATCATTACTTCAAAACT3310.10122819465172607No Hit
GTTCATATATTGTTTACACAGAAAAGCCTTGTAAGTTGGCGAGAAAGTGA3300.1009223692902405No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT3290.10061654392875494No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)