FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326984
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTACATGGTCCGG22950.7018692046093998No Hit
CTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT18320.5602720622415776No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG15830.4841215472316689No Hit
CTCTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT15420.4715827074107602No Hit
GTGTTGAAGACCAACCTCCCATGCTGTAGCTCTTGTTCCCAAGAATATGG15190.46454872409659187No Hit
TAGACAAAGGCATTAAACCTTATTATCCGGACCATGTAGTTAATCATTAC14560.4452817263230005No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11180.3419127541408754No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9880.30215545714775033No Hit
CTTTATCTAAGGGCAAATATTTGGTAAGGTTAGGATAAAACCTAGCAGGC9830.3006263303403225No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT9620.2942039977491253No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9270.28350011009713016No Hit
CATACAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA9070.2773836028674186No Hit
ATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATG8880.2715729209991926No Hit
CTTCCACACCTCGAAAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCT8860.27096127027622147No Hit
ATTTTGTGGTCTGTAAGCGGGAGGAGTGCGAATCCACACTCCAAAAGACA8800.26912631810730797No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT8530.2608690333471974No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA8240.2520000978641157No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8210.25108262177965895No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTACATGGTCC7910.24190786093509165No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTACA7900.24160203557360604No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC7800.23854378195875026No Hit
ACAATATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAA7730.23640300442835122No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA7270.22233503780001465No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC6970.21316027695544737No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA6500.19878648496562523No Hit
CTCCAAAAGACACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGA6350.19419910454334155No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6340.193893279181856No Hit
GTCCCGTACTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6280.19205832701294254No Hit
GTACAAAAACTCAAGCAATGTTTTCGCAAACTTCCTGTAAATAGACCTAT6170.18869424803660118No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG6120.1871651212291733No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATGTCCTCCTGC5990.1831893915298608No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAAGTTAAGGGAG5880.17982531255351944No Hit
CCCTTAACTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG5840.17860201110757715No Hit
GAAATTTCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGCTTGTATA5680.1737088053238079No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTGCAGG5660.17309715460083674No Hit
GTACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5580.17065055170895213No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAA5570.17034472634746653No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC5450.16667482200963962No Hit
CTCACCAACCTCTTGTCCTCCAATTTGTCCTGGCTATCGTTGGATGTGTC5420.1657573459251829No Hit
TATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATGAAAAAA5300.16208744158735597No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTAT5270.16116996550289922No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5250.16055831477992807No Hit
CTTTATATGCATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACT5210.15933501333398578No Hit
GTGTAGTTTCTCTCTTATATAGAATACCAGCCTTCCACAGAGTATGTAAA5090.15566510899615885No Hit
GTTTTAGTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA5030.15383015682724538No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA5030.15383015682724538No Hit
CAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATGAAAAAAGGA4910.15016025248941844No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAACCTTAT4770.14587869742862036No Hit
GCCTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAAC4710.1440437452597069No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAAGCCTTGTAAGTTGGCGA4690.14343209453673575No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4670.1428204438137646No Hit
GCATTAAACCTTATTATCCGGACCATGTAGTTAATCATTACTTCAAAACT4660.142514618452279No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC4650.14220879309079343No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC4550.13915053947593767No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTA4530.1385388887529665No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4490.1373155873070242No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT4480.1370097619455386No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4400.13456315905365399No Hit
ATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTTGA4370.13364568296919727No Hit
CCTTTATCTAAGGGCAAATATTTGGTAAGGTTAGGATAAAACCTAGCAGG4350.13303403224622612No Hit
ATATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTA4290.13119908007731265No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT4170.12752917573948575No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG4170.12752917573948575No Hit
GTGTGGAAGACCAACCTCCCATGCTGTAGCTCTTGTTCCCAAGAATATGG4140.126611699655029No Hit
GTTGTCAGTATGCCCTGAGTCTGAGGGCTCCACCCCAAAAGACCGCCGTG4130.12630587429354342No Hit
GTTTTATGCCTTTGTCTAAGGGCAAATATTTGGTAAGGTTAGGATAAAAC4130.12630587429354342No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4090.1250825728476011No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA4080.12477674748611553No Hit
GCGTGTAGTTTCTCTCTTATATAGAATACCAGCCTTCCACAGAGTATGTA4070.12447092212462996No Hit
GTCTTTGAAGTACGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA4040.12355344604017324No Hit
GAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAA4030.12324762067868764No Hit
GTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTAAGGTTAGGATAAAAC4020.12294179531720208No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA3930.12018936706383186No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT3930.12018936706383186No Hit
ATTTGGTGGTCTGTAAGCGGGAGGAGTGCGAATCCACACTCCAAAAGACA3870.11835441489491841No Hit
ACCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT3850.11774276417194725No Hit
ACTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCACCACCAATCGGCAGT3810.11651946272600495No Hit
ATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATGAAAAAAG3790.1159078120030338No Hit
GTACTAAAACTCAAGCAATGTTTTCGCAAACTTCCTGTAAATAGACCTAT3760.11499033591857706No Hit
GAAAAGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGCTTGTATA3740.1143786851956059No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT3730.11407285983412033No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCATATTTCC3730.11407285983412033No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCT3690.11284955838817802No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG3690.11284955838817802No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG3690.11284955838817802No Hit
GTTTTGAAGTAATGATTAACTACATGGTCCGGATAATAAGGTTTAATGCC3680.11254373302669245No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA3640.11132043158075013No Hit
ATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAG3630.11101460621926455No Hit
GTTCATATATTGTTTACACAGAAAAGCCTTGTAAGTTGGCGAGAAAGTGA3600.11009713013480783No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC3590.10979130477332223No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC3560.10887382868886551No Hit
GGACATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATG3540.10826217796589435No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT3540.10826217796589435No Hit
CCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCAA3430.104898098989553No Hit
GGGTTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGGAAGAT3370.10306314682063955No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG3360.10275732145915396No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTGTATGCATGTATA3350.10245149609766839No Hit
GCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA3350.10245149609766839No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTCTATATAAGAGAGAAACTACA3300.1009223692902405No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTACTGGTAGTTGATGTTCCTGG3300.1009223692902405No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA3290.10061654392875494No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)