FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486113
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC25720.529095086944805No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC22440.46162106341529646No Hit
ACTCACAACTGTGCCAGCGGCTCCTCCTCCTGCCTCCACCAATCGGCAGT21910.4507182486376624No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA21400.44022686083276935No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG17130.3523872021525859No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16360.336547263702061No Hit
CCCAAAGTCAGGGCCCTGTACTTTCCTGCTGGTGGCTCCAGTTCAGGAAC15840.3258501624107975No Hit
GGTTAGTGAGTGACTGGAGATTTGGGACTGCGAATTTTGGCCAAGACACA15200.3126844992830885No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATCAACTACA15040.30939308350116124No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT15040.30939308350116124No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA14980.30815880258293854No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12710.2614618411768457No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT12690.2610504142041048No Hit
TTTGCAGGAGGACATTGTTGATAGATGTAAGCAATTTGTGGGGCCCCTTA12150.24994188594010033No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT11530.23718764978513224No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT11420.23492480143505728No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG11140.2291648238166846No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG11140.2291648238166846No Hit
CCTTAGGCAGGTTCCCGTGGGCGTTCACGGTGGTTTCCATGCGACGTGCA11100.2283419698712028No Hit
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC11030.22690197546660962No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT10870.22361055968468235No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT9580.19707351994289393No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG9360.19254782324274397No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTAAGGCA9020.18555356470614856No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA9020.18555356470614856No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA8940.18390785681518496No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC8890.1828792893833327No Hit
CTCACCAACCTGTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTC8880.18267357589696223No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT8730.17958787360140543No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA8530.17547360387399638No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA8160.16786220487828962No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG8140.1674507779055487No Hit
GACTCTGGGATCTTGAAGAGTTTGGTGGAAAGTGGTGGAGTTCCACTGCA8120.1670393509328078No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC8070.16601078350095555No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA7890.1623079407462874No Hit
CGTGTGGACTCTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAA7870.16189651377354647No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA7810.16066223285532377No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG7720.1588108114779897No Hit
ACCTTAGGCAGGTTCCCGTGGGCGTTCACGGTGGTTTCCATGCGACGTGC7670.15778224404613742No Hit
CGTTAATATGGGCCTAAAACTCAGACAATTATTGTGGTTTCACATTTCCT7660.15757653055976695No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCAC7590.15613653615517378No Hit
GTATGTAGTTGATCATTACTTCCAGACGCGACATTATTTACACACTCTTT7480.15387368780509883No Hit
TATCCACATTGTGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG7370.15161083945502385No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG7320.15058227202317157No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG7280.14975941807768975No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC7040.14482229440479888No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCT7010.14420515394568753No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA6920.14235373256835346No Hit
GTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG6880.14153087862287164No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT6740.13865088981368529No Hit
TCGCAGAAGATCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACA6610.1359766144908694No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTGAAA6610.1359766144908694No Hit
ACCACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGGGG6570.13515376054538758No Hit
AATTAGAGGACAACAGGTTGGTGAGTGACTGGAGATTTGGGACTGCGAAT6530.13433090659990576No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA6510.13391947962716488No Hit
ATATTAACGTTGACATAGCCGACTACTAATTCCCTGGATGCTGGATCTTC6460.1328909121953126No Hit
GTATTATCCAGAGTATGTAGTTGATCATTACTTCCAGACGCGACATTATT6430.13227377173620125No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT6390.13145091779071943No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT6370.13103949081797853No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGC6340.13042235035886718No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA6320.13001092338612627No Hit
GCCTATGGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCC6290.1293937829270149No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT6260.12877664246790355No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATCAACTACATACTCTGGAT6210.1277480750360513No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6190.12733664806331038No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC6170.12692522109056947No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC6130.12610236714508766No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC6110.12569094017234675No Hit
GTGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCGTTGACATACT6080.1250737997132354No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTA6070.12486808622686496No Hit
CCTCCAATTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGTTTTATCAT6050.12445665925412404No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT6040.12425094576775358No Hit
TTAACGCAGGATATCCACATTGTGTGAAAGGGGCGGCAAACCCCAAAAGA6000.12342809182227178No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC5880.12095952998582633No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA5860.12054810301308544No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5840.12013667604034452No Hit
CTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACT5820.11972524906760362No Hit
ATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA5800.1193138220948627No Hit
TCCTAGTACAAAGACCTTTAACCTCATCTCCTCCCCCAACTCCTCCCACT5770.11869668163575135No Hit
ACCTTGGGCAGGTTCCCGTGGGCGTTCACGGTGGTTTCCATGCGACGTGC5760.11849096814938091No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC5740.11807954117663999No Hit
GTACAGGGTCCCCAGTCTTCGATAAGATTGACGATATGGCAGAGACAGTA5650.11622811979930593No Hit
CTTTCACACAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA5470.11252527704463777No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA5430.11170242309915596No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5410.11129099612641506No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA5400.1110852826400446No Hit
GCACAACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACA5370.11046814218093325No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5300.10902814777634007No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG5290.10882243428996961No Hit
TAGATAAAGGGATCAAACCGTATTATCCAGAGTATGTAGTTGATCATTAC5270.10841100731722872No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA5270.10841100731722872No Hit
TTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC5240.10779386685811736No Hit
CTTTATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGC5190.10676529942626509No Hit
ACCCTGTACCGAACATGGAGAACATCGCATCAGGACTCCTAGGACCCCTG5180.10655958593989462No Hit
GGTCCTAGGAGTCCTGATGCGATGTTCTCCATGTTCGGTACAGGGTCCCC5130.10553101850804238No Hit
GTCCTGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC5120.10532530502167191No Hit
CACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGCACCTTGC5100.10491387804893101No Hit
GGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA5080.10450245107619009No Hit
CTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACA5060.1040910241034492No Hit
GCTTTATTCTTCTACGGCACCTTGCTTTAATCCTAAATGGCAAACTCCTT4990.10265102969885603No Hit
AAACAAAGGACGTCCCGCGCAGGATCCAGTTGGCAGCACAGCCTAGCAGC4950.10182817575337422No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT4930.10141674878063331No Hit
GGCCTGGTCTGTGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGC4920.10121103529426285No Hit
CATTTGCAGGAGGACATTGTTGATAGATGTAAGCAATTTGTGGGGCCCCT4920.10121103529426285No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT4890.10059389483515149No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)