FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486113
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC23640.4863066817797508No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA20020.4118383997136469No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG18810.386947067862822No Hit
ACTCACAACTGTGCCAGCGGCTCCTCCTCCTGCCTCCACCAATCGGCAGT18680.3842727925400061No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT16360.336547263702061No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16240.3340787018656156No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATCAACTACA15470.3182387634150907No Hit
GGTTAGTGAGTGACTGGAGATTTGGGACTGCGAATTTTGGCCAAGACACA15150.31165593185123625No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA15050.3095987969875317No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC14640.30116454404634313No Hit
CCCAAAGTCAGGGCCCTGTACTTTCCTGCTGGTGGCTCCAGTTCAGGAAC13830.2845017516503364No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT12780.26290183558143887No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12460.2563190040175844No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG12130.24953045896735945No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT11440.2353362284077982No Hit
TTTGCAGGAGGACATTGTTGATAGATGTAAGCAATTTGTGGGGCCCCTTA11290.2322505261122414No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT11020.22669626198023918No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT10480.21558773371623471No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA10250.21085632352971428No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG9920.20406777847948934No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC9350.1923421097563735No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA9040.18596499167888947No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT9030.18575927819251903No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTAAGGCA9010.18534785121977812No Hit
GTATGTAGTTGATCATTACTTCCAGACGCGACATTATTTACACACTCTTT8990.1849364242470372No Hit
CCTTAGGCAGGTTCCCGTGGGCGTTCACGGTGGTTTCCATGCGACGTGCA8810.18123358149236907No Hit
CTCACCAACCTGTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTC8590.17670788479221908No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA8340.17156504763295777No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG8120.1670393509328078No Hit
GTATTATCCAGAGTATGTAGTTGATCATTACTTCCAGACGCGACATTATT8100.1666279239600669No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCT8050.16559935652821464No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA8040.16539364304184417No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG8010.16477650258273283No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA7980.16415936212362145No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA7970.163953648637251No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT7950.1635422216645101No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA7920.16292508120539875No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT7780.1600450923962124No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC7680.15798795753250788No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC7590.15613653615517378No Hit
GACTCTGGGATCTTGAAGAGTTTGGTGGAAAGTGGTGGAGTTCCACTGCA7570.1557251091824329No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA7560.15551939569606243No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG7400.1522279799141352No Hit
GTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG7290.14996513156406022No Hit
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC7230.1487308506458375No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC7000.1439994404593171No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATCAACTACATACTCTGGAT6890.14173659210924208No Hit
CGTTAATATGGGCCTAAAACTCAGACAATTATTGTGGTTTCACATTTCCT6870.14132516513650117No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA6750.13885660330005575No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG6680.1374166088954626No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG6680.1374166088954626No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCAC6610.1359766144908694No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC6550.1347423335726467No Hit
GCCTATGGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCC6420.13206805824983078No Hit
GTGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCGTTGACATACT6290.1293937829270149No Hit
ACCACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGGGG6230.1281595020087922No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT6220.12795378852242173No Hit
CTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACT6200.12754236154968085No Hit
CCTCCAATTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGTTTTATCAT6200.12754236154968085No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT6140.12630808063145813No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC6020.12383951879501268No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTGAAA5960.12260523787678997No Hit
CGTGTGGACTCTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAA5920.12178238393130815No Hit
TATCCACATTGTGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG5910.1215766704449377No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT5880.12095952998582633No Hit
GTACAGGGTCCCCAGTCTTCGATAAGATTGACGATATGGCAGAGACAGTA5800.1193138220948627No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC5790.11910810860849227No Hit
AATTAGAGGACAACAGGTTGGTGAGTGACTGGAGATTTGGGACTGCGAAT5750.11828525466301046No Hit
GCCTAAGGTCTTGCATAAGAGGACTCTTGGACTTTCAGCAATGTCAACGA5750.11828525466301046No Hit
ATATTAACGTTGACATAGCCGACTACTAATTCCCTGGATGCTGGATCTTC5740.11807954117663999No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT5660.11643383328567639No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA5650.11622811979930593No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA5610.11540526585382412No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA5560.11437669842197183No Hit
CTTTATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGC5530.11375955796286048No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA5520.11355384447649004No Hit
GGCCTGGTCTGTGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGC5510.11334813099011959No Hit
GGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA5510.11334813099011959No Hit
CTTTCACACAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA5460.1123195635582673No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGC5430.11170242309915596No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTA5420.11149670961278552No Hit
TCGCAGAAGATCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACA5350.11005671520819234No Hit
GCACAACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACA5310.10923386126271054No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA5280.10861672080359917No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5250.1079995803444878No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC5250.1079995803444878No Hit
GCTTTGGGACATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5220.10738243988537644No Hit
GCCCAAGGTCTTGCATAAGAGGACTCTTGGACTTTCAGCAATGTCAACGA5160.10614815896715372No Hit
ACCTTAGGCAGGTTCCCGTGGGCGTTCACGGTGGTTTCCATGCGACGTGC5120.10532530502167191No Hit
ATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA5100.10491387804893101No Hit
GTCCTGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC5070.10429673758981965No Hit
TTAACGCAGGATATCCACATTGTGTGAAAGGGGCGGCAAACCCCAAAAGA4970.10223960272611513No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC4940.10162246226700375No Hit
CATTTGCAGGAGGACATTGTTGATAGATGTAAGCAATTTGTGGGGCCCCT4930.10141674878063331No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGATGA4890.10059389483515149No Hit
ACCCTGTACCGAACATGGAGAACATCGCATCAGGACTCCTAGGACCCCTG4880.10038818134878104No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)