FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489832

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489832
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences513443
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA27510.5357946256936018No Hit
CTATCAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG21990.42828512609968383No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT20680.4027710963047505No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG20460.39848629740789143No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC18900.36810317795743636No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAACCGTAT18490.36011787092238084No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA17450.3398624579554108No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17200.3349933682998892No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA15550.30285737657344636No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG15420.30032544995257504No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCATCTGTGGA14450.2814333820891511No Hit
TTATTGATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA13890.2705266212607826No Hit
GACCTTTAACCTAAACTCCTCCCCCAACTCCTCCCAGTCTTTAAACACAC13460.26215178705328535No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA13040.253971716432009No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT12980.2528031349146838No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG12650.24637593656939524No Hit
CATCCAGCGATAGCCAGGACAAATTGGAGGACAAGAGGTTGGTGAGTGAT12560.24462306429340747No Hit
GTTCAGGATAATACGGTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTA12050.2346901213961433No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA11780.2294315045681799No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC11420.22242001546422877No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT11400.222030488291787No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10840.21112372746341854No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA10610.2066441649803386No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC10350.2015803117385961No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT10290.2004117302212709No Hit
CCCACGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC10170.19807456718662053No Hit
GGATAATACGGTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTAAGGTT10010.19495834980708665No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG9930.19340024111731974No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9730.18950496939290243No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT9410.18327253463383472No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA9380.18268824387517213No Hit
GACCTGCTGCGAGCAAAACAAGCTGCTAGGAGTTCCGCAGTATGGATCGG9360.1822987167027304No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG9300.1811301351854052No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCATCTGTGG9220.17957202649563828No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC9120.1776243906334296No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGCTCACCTCACCATACAG9070.1766505727023253No Hit
GTTCCGGATAATACGGTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTA8950.1743134096676749No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8870.17275530097790795No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT8700.16944432001215326No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAAC8620.16788621132238632No Hit
CGCGAGGTCCAACTGGTGATCGGGAAAGAATCCCAGAGGATTGGGAACAG8580.16710715697750284No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC8500.16554904828773592No Hit
CATTTAAACCCTAATAAAACCAAGCGTTGGGGCTACTCCCTTAACTTCAT8400.16360141242552728No Hit
GACCAGACCGTTGCCGGGCAACGGGGTAAAGGTTCAGATATTGTTTACAC8350.16262759449442293No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG8300.1616537765633186No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCATCTGTGG8060.15697945049401785No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG7860.1530841787696005No Hit
GGGTAAAGGTTCAGATATTGTTTACACAAAAAGGCCTTGTAAGTTGGCGA7720.1503574885625084No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG7590.14782556194163715No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC7390.14393029021721981No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA7380.14373552663099898No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA7350.14315123587233639No Hit
GCCTTAGAGTCTCCGGAACATTGCTCACCTCACCATACAGCACTCAGGCA7330.14276170869989463No Hit
GGTCAGGTCTCTGCCAAGTGTTTGCTGACGCAACCCCCACTGGATGGGGC7160.1394507277341399No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT7110.1384769098030356No Hit
CTACTGTACCTGTCTTTAATCCCGAGTGGCAAACTCCCTCCTTTCCTCAC7090.13808738263059386No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT7040.13711356469948952No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA6990.1361397467683852No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6840.1332182929750722No Hit
GATCATTAACCTAAACTCCTCCCCCAACTCCTCCCAGTCTTTAAACACAC6610.1287387304919923No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA6590.12834920331955055No Hit
CCCAAGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC6550.1275701489746671No Hit
GGAATCGTGCAGGTCTTGCATGGCCCCGTGCTGGTGGTTGATGTTCCTGG6530.1271806218022254No Hit
CCTAAACTCCTCCCCCAACTCCTCCCAGTCTTTAAACACACAGTCTTTGA6510.12679109462978363No Hit
ACACTTGGGTGCTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTA6420.12503822235379586No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC6380.12425916800891239No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC6350.1236748772502498No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC6290.12250629573292458No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG6220.12114295062937853No Hit
CCTTTTCGAGGTTTGGAAGACCAACCTCCCATGCTGTAGCTCTTGTTCCC6170.1201691326982742No Hit
CAGCCGGTCTGGAGCGAACCTTATCGGAACCGACAACTCTGTTGTCCTCT6120.11919531476716988No Hit
GTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAAC6030.11744244249118209No Hit
GACCTGACCGTTGCCGGGCAACGGGGTAAAGGTTCAGATATTGTTTACAC5890.11471575228408995No Hit
GATCCTTGGCCAGAGGCAAATCAGGTAGGAGCGGGAGCATTCGGGCCAGG5870.11432622511164824No Hit
GTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT5850.1139366979392065No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC5780.11257335283566045No Hit
GTCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATAA5750.11198906207699783No Hit
TATTGATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA5730.1115995349045561No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5710.11121000773211438No Hit
GACCCTGCGTTCGGAGCCAACTCAAACAATCCAGATTGGGACTTCAACCC5670.11043095338723091No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5500.10711997242147618No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT5440.105951390904151No Hit
CTTTAATCCCGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG5430.10575662731793012No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5420.10556186373170927No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC5330.10380899145572148No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT5300.10322470069705889No Hit
TTCTTCTACTGTACCTGTCTTTAATCCCGAGTGGCAAACTCCCTCCTTTC5230.10186135559351281No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA5230.10186135559351281No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG5220.10166659200729194No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5180.10088753766240847No Hit
GGCCAACCACGGGGCGCACCTCTCTTTACGCGGTCTCCCCGTCTGTGCCT5160.10049801048996675No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5150.10030324690374588No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)