FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489834

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489834
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501034
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA22770.45446017635529723No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC19050.38021371803111165No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT16600.3313148409090002No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA12340.24629067089259413No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT12310.2456919091319152No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT11300.22553359652239172No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA11290.22533400926883204No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT11000.21954597891560257No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG9150.18262233700706937No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC9130.1822231624999501No Hit
ATCTTAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG8650.17264297432908743No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT8330.16625618221517902No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT8220.164060722426023No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC8210.16386113517246334No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT8200.16366154791890372No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA8130.16226443714398622No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA8040.1604681518619495No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG8010.15986939010127058No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT7890.15747434305855493No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG7840.15647640679075672No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG7770.15507929601583925No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG7750.15468012150872No Hit
CATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT7680.1532830107338025No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGGTATCCACATTGCGTGAAA7630.1522850744660043No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA7360.14689621861989408No Hit
GTGGATACCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAACAG7310.14589828235209587No Hit
CTCATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA7180.14330364805582055No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA7070.14110818826666452No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT7060.1409086010131049No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC6790.13551974516699464No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC6680.1333242853778386No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCTAAACAGGCTTTTACTTT6650.1327255236171597No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT6650.1327255236171597No Hit
CCTCCCGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6570.1311288255886826No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6560.130929238335123No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6540.13053006382800367No Hit
GCACCGGACCATGCAAAACCTGCACAGCTCCTGCTCAAGGAACCTCTATG6510.12993130206732478No Hit
GTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGCAAGACCTTGGG6290.12554038248901273No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT6280.12534079523545308No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6140.12254657368561814No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT6080.12134905016426031No Hit
CCTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCT6070.12114946291070068No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG6060.12094987565714103No Hit
GCTTTAGGGCATGGACATTGACACCTATAAAGAATTTGGAGCTTCTGTGG5740.1145630835432326No Hit
CTCTTATGCAAGACCTTGGGCAGGTTCCTGTGGGCGTTCACGGTGGTCTC5740.1145630835432326No Hit
CACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAACCT5660.11296638551475549No Hit
TCCCGATACAGCGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA5660.11296638551475549No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGC5650.11276679826119586No Hit
TCCTAGTACAAAGACCTTTAACCTAACCTCCGCCCCCAACTCCTCCCACT5640.11256721100763621No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT5620.11216803650051692No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAGGTTCCTGTGGGCGTTC5570.11117010023271874No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGTAGAA5490.10957340220424162No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA5380.10737794241508561No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC5370.10717835516152598No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5350.10677918065440668No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC5340.10657959340084704No Hit
CTCTAAGGCCTCCCGATACAGCGCAGAGGCGGTGTCGAGGAGATCTCGAA5330.1063800061472874No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5310.10598083164016812No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG5310.10598083164016812No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG5280.10538206987948923No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT5270.10518248262592958No Hit
ATATTAACGTTGACATAGCCGACTACTAATTCCCTGGATGCTGGATCTTC5260.10498289537236995No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC5240.10458372086525067No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5160.10298702283677355No Hit
CGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCCT5150.10278743558321392No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA5130.10238826107609464No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA5080.10139032480829643No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT5040.10059197579405789No Hit
TCTCCATGCTACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGAT5020.10019280128693861No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)