FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences530521
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTGGTTTTGAAGTAATGATTAACTACATGTTCAGG22010.41487518872956963No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTGGTTT18940.35700754541290547No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17580.33137236791757535No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAACCATAT17310.3262830312089437No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA17040.32119369450031193No Hit
CCCTTAACTTCATGGGATATGTAATTGGATGTTGGGGTACTTTACCGCAA15100.2846258677790323No Hit
GTATACATGCGTATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA12970.2444766559664933No Hit
ATGTAATTGGATGTTGGGGTACTTTACCGCAAGAACATATTGTACAAAAA12800.2412722587795771No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT12370.2331670188362006No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG12050.2271352123667112No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTGGT11970.22562726074933886No Hit
CCCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATCATGCCTGCAAGG11810.22261135751459413No Hit
GGATAATATGGTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTAAGGTT11540.21752202080596245No Hit
GTCTATTGACAGGCAGTTTTCGAAAACATTGCTTGATTTTTTGTACAATA11480.2163910570929332No Hit
GGTTAGGATAGAACCTTGCAGGCATGATTAATTTTAATCTCCTTTTTTCA11250.21205569619298764No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGCCCCACAATTCTCTGACATACT11220.21149021433647303No Hit
CTCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAG10880.2050814199626405No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC10340.1949027465453771No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAAC9940.18736298845851532No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC9790.18453557917594213No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT9720.18321612151074135No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9710.18302762755856977No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG9710.18302762755856977No Hit
CAATAGGTCTATTGACAGGCAGTTTTCGAAAACATTGCTTGATTTTTTGT9530.17963473641948197No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC9520.17944624246731045No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA9300.17529937551953645No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATGGCTGAGAGTCCAA9080.17115250857176248No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG8960.16889058114570393No Hit
GGTCTATTGACAGGCAGTTTTCGAAAACATTGCTTGATTTTTTGTACAAT8890.16757112348050313No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG8890.16757112348050313No Hit
CTGACATACTTTCCAATCAATAGGTCTATTGACAGGCAGTTTTCGAAAAC8820.16625166581530232No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATACGCATGTATA8630.16267028072404296No Hit
GTTAATAGATGTCAACAATATGTGGGCCCCCTTACAGTTAATGAAAAAAG8430.15890040168061206No Hit
TTGTTAATAGATGTCAACAATATGTGGGCCCCCTTACAGTTAATGAAAAA8290.15626148635021045No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8240.15531901658935274No Hit
CATTTGAACCCTAATAAAACCAAGCGGTGGGGCTACTCCCTTAACTTCAT7840.14777925850249096No Hit
GTTCAGGATAATATGGTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTA7730.14570582502860396No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCCATGTCAACGACCGACCT7610.14344389760254542No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT7540.1421244399373446No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTACTGTGGTTTCACATTTCC7240.13646962137219826No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC7170.13515016370699745No Hit
GTCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTTGCAGGCATGA7120.13420769394613974No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA6920.13043781490270887No Hit
GGATAGAACCTTGCAGGCATGATTAATTTTAATCTCCTTTTTTCATTAAC6920.13043781490270887No Hit
ATATGTTCTTGCGGTAAAGTACCCCAACATCCAATTACATATCCCATGAA6910.1302493209505373No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA6690.12610245400276332No Hit
GAGTATGTAAATAATGCCTGGTTTTGAAGTAATGATTAACTACATGTTCA6670.12572546609842022No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6660.12553697214624868No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA6650.12534847819407716No Hit
CAATTACATATCCCATGAAGTTAAGGGAGTAGCCCCACCGCTTGGTTTTA6570.12384052657670479No Hit
AGTTAAGGGAGTAGCCCCACCGCTTGGTTTTATTAGGGTTCAAATGTATA6560.12365203262453325No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA6470.12195558705498935No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6470.12195558705498935No Hit
GGATATGTAATTGGATGTTGGGGTACTTTACCGCAAGAACATATTGTACA6340.11950516567675927No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC6260.1179972140593869No Hit
TAATAAAACCAAGCGGTGGGGCTACTCCCTTAACTTCATGGGATATGTAA6240.1176202261550438No Hit
ATATTATCCTGAACATGTAGTTAATCATTACTTCAAAACCAGGCATTATT6230.11743173220287227No Hit
GTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAAC6220.11724323825070074No Hit
TCCACAGAGTATGTAAATAATGCCTGGTTTTGAAGTAATGATTAACTACA6180.11648926244201455No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT6140.11573528663332837No Hit
GTCGACGAGATCTCGAATAGAAGGAAAGAAGTCAGAAGGCAAAAAAGAGA6050.11403884106378447No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6000.11309637130292674No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5990.1129078773507552No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA5950.11215390154206903No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGCTCACCTCACCATACAG5880.11083444387686821No Hit
ATATGGTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTAAGGTTAGGAT5860.11045745597252513No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC5820.10970348016383893No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG5730.10800703459429503No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT5730.10800703459429503No Hit
GTCAATAGACCTATTGATTGGAAAGTATGTCAGAGAATTGTGGGGCTTTT5720.1078185406421235No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5710.10763004668995196No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA5680.10706456483343732No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5670.10687607088126579No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC5650.10649908297692269No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5630.1061220950725796No Hit
CATTTACAGGAGGACATTGTTAATAGATGTCAACAATATGTGGGCCCCCT5620.10593360112040806No Hit
GTTTGGGACTCTACCGTCCACTTCTTCGTCTGCCGTTCCGGCCGACCACG5590.10536811926389342No Hit
GATTGTATACATGCGTATAAAGGCATTAAGGCAGGATAGCCACATTGTGT5490.10348317974217798No Hit
GAATTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACA5440.10254070998132025No Hit
GGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG5360.10103275836394789No Hit
GTATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCAA5360.10103275836394789No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)