FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489838

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489838
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422776
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTCGTTTGGAAGTAATGATTAACTATGTGTTCAGG17110.4047060381857059No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA15190.35929191817889383No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT14450.3417885594262683No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11350.26846367816526956No Hit
GTGTAAAGGGAGCGGCAAAGCCCAAAAGCCCCACAATACGTTGACATACT11320.2677540825401631No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG10920.25829280753874395No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG10640.2516699150377505No Hit
CATGTATACAAGCCAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC10500.24835846878725376No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT10160.24031638503604746No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTCACTTCAT9570.22636100440895415No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9060.21429787878214468No Hit
GGAATATGATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAATT8820.20862111378129317No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8310.1965579881544837No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTT8300.1963214562794482No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT8280.19584839252937725No Hit
ATGTAAGACCTTGGGCAAGACCTGTCGGGCGTTCACGGTGGTCTCCATGC8080.19111775502866768No Hit
CTTTCGGAGTGTGGATTCGCACTCCTACCGCTTACAGACCACCAAATGCC8000.18922550002838384No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA7720.1826026075273904No Hit
GGATATTATTAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG7710.1823660756523549No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATG7610.18000075690200013No Hit
ATTTACAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT7550.17858156565178723No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT7460.17645277877646792No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA7240.17124907752568735No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG7190.17006641815050996No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT7060.16699150377504873No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGA7010.16580884439987134No Hit
CTCCGAAAGACACCAAATACTCAAGAACAGTATCTCTTCCAAAAGTAAGA6810.16107820689916175No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC6670.15776676064866502No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC6550.15492837814823926No Hit
CTCAAGAACAGTATCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA6500.15374571877306184No Hit
ATCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT6490.15350918689802637No Hit
ATCCAGCATCCAGAGAATTAGTAGTAAGCTATGTCAATGTTAACATGGGC6440.15232652752284898No Hit
GACCAAACCGTTGCCGAGCAACGGGGTAAAGGTTCAGATATTGTGTACGC6370.15067080439760064No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACATAAATC6280.1485420175222813No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6200.14664976252199746No Hit
TTATTAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAA6050.14310178439646526No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC5980.14144606127121692No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5960.14097299752114595No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC5930.1402634018960395No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAACGTATTGTGGGGCT5900.13955380627093308No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT5830.13789808314568472No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA5830.13789808314568472No Hit
GTAGTAAGCTATGTCAATGTTAACATGGGCCTAAAAATCAGACAACTATT5750.13600582814540088No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5410.12796374439419456No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5380.12725414876908814No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA5320.12583495751887525No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC5220.12346963876852045No Hit
TCCACAGAGTATGTAAATAATGCCTCGTTTGGAAGTAATGATTAACTATG5190.122760043143414No Hit
TCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG5180.12252351126837853No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA5160.12205044751830757No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTAATAATATCCTCTTTC5100.12063125626809469No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5080.12015819251802373No Hit
GACTTCTTTCCTTCTATTCGTGATCTCCTCGACACCGCCTCTGCTCTGTA5050.11944859689291729No Hit
GAGTATGTAAATAATGCCTCGTTTGGAAGTAATGATTAACTATGTGTTCA4980.11779287376766892No Hit
ATACCACATCATCCATATAACTGAAAGCCAAGCAGTGGGGGAAAGCCCTA4880.11542755501731414No Hit
GGGTAAAGGTTCAGATATTGTGTACGCAGAAAGGCCTTGTAAGTTGGCGA4880.11542755501731414No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTCGTTT4750.1123526406418529No Hit
GAATTAGTAGTAAGCTATGTCAATGTTAACATGGGCCTAAAAATCAGACA4680.11069691751660454No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTCGT4660.11022385376653358No Hit
ATGTTAACATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC4640.10975079001646262No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGTCGGGCGTTC4610.10904119439135618No Hit
TATTAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAA4530.10714893939107234No Hit
ATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG4520.10691240751603687No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGTATGCGCTGATGGCC4450.1052566843907885No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTAGTG4430.10478362064071753No Hit
ATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCCAGTC4410.10431055689064658No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC4350.10289136564043372No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4350.10289136564043372No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTGAAGGGAG4340.10265483376539822No Hit
CTCCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG4340.10265483376539822No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCCAAACAGGCTTTCACTTT4320.10218177001532726No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAAT4300.10170870626525631No Hit
TCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTT4260.10076257876511438No Hit
TCACCATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA4250.10052604689007891No Hit
ATGCTGGATCGTCCAAATTACTTCCCACCCAGGTGGCCAGATTCATCAAC4240.10028951501504343No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)