FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences431071
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN69951.6227025246421123No Hit
CCCTTTACACAATGTGGATACCCAGCATTAATGCCTTTGTATGCATGTAT9130.211798056468656No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG8420.19532745185827857No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8310.1927756680454032No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG7830.18164061140740156No Hit
GTGTAAAGGGAGCGGCAAAACCCAAAAGTCCCACAATTCTTTGACATACT7530.17468120100865056No Hit
GTTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG7370.17096951546265No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6950.16122634090439858No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6770.15705069466514796No Hit
ATTTATAGGAAGTTTCCTAAAACATTGTTTGATTTTTTGTACAATATGTT6720.1558907929320228No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTGAGA6520.15125118599952211No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6470.15009128426639695No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA6420.1489313825332718No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6360.14753950045352157No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGACT6200.14382781490752103No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTATCCACATTGTGTAAAG5710.13246077792289437No Hit
GCCTAGCAGCCATGGAAAAGAGGTGTATTTCCGAGAGAGGACAACAGAGT5490.1273572102971436No Hit
ATCCTAAACTTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT5310.12318156405789302No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG5150.11946987851189246No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG5040.11691809469901711No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTT5010.11622215365914199No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTA4960.11506225192601682No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT4950.1148302715793918No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4930.11436631088614173No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC4840.11227848776651642No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG4740.10995868430026608No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTATTCG4720.10949472360701601No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTT4510.10462313632789029No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)