FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489843

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489843
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493097
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATATAGAATGCCAGCCTTCCAAAGGGTATGCAAATAATGCCTAGTTT23940.48550285237995766No Hit
TTATTAATAGATGTCAACAATTTGTGGGCCCTCTAACTACAAATGAAAAA22660.4595444709661588No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20620.4181733005879168No Hit
GTGCTGGTACGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC19160.3885645217878024No Hit
CTATTAATAATGTCCTCCTGCAAATGTATATCAGGAAAAGAGGGAGTTTG15630.3169761730450601No Hit
CCTTTATCTAATGGCAAATATTTGGTAAGGTTAGGAAAAAATCTAGCGGG15620.31677337319026483No Hit
CTCTTATATAGAATGCCAGCCTTCCAAAGGGTATGCAAATAATGCCTAGT15240.3090669787080432No Hit
GTCTTTGAAGTACGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA14610.2962905878559391No Hit
GTTTAATACCTTTATCTAATGGCAAATATTTGGTAAGGTTAGGAAAAAAT14390.2918289910504424No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT14260.28919259293810345No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG13430.2723602049900932No Hit
GGATAATAAGGTTTAATACCTTTATCTAATGGCAAATATTTGGTAAGGTT13090.2654650099270529No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12710.2577586154448313No Hit
CTCTAACTACAAATGAAAAAAGGAGATTAAAGTTAATTATGCCCGCTAGA10840.2198350425981095No Hit
CCCCCCGCATACAGGCCACCAAATGCCCCTATCTTATCAACACTTCCGGA10540.21375104695425037No Hit
TCCAAAGGGTATGCAAATAATGCCTAGTTTTGAAGTAATGATTAACTGTA10340.2096950498583443No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCTTTTC10220.20726145160080067No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA9800.1987438576993979No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCTTTTCCTGATATACATTTGCAGG9600.1946878606034918No Hit
GTATTAAACCTTATTATCCTGAACATACAGTTAATCATTACTTCAAAACT9570.1940794610391059No Hit
GTATACATGCATACAAAGGCAATAAGGCAGGATAACCACATTGTGTAAAA9560.1938766611843106No Hit
GTTTTATTAGGGTTCAAATGGATACCCAAAGATAAAAGAAAATTGGTAAC9540.19347106147471999No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA9470.19205146249115285No Hit
CCCTAAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG9190.18637306655688435No Hit
GTTCAGGATAATAAGGTTTAATACCTTTATCTAATGGCAAATATTTGGTA8960.18170866989659237No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTGTTACCAATTTTCTT8890.18028907091302523No Hit
TTATTATCCTGAACATACAGTTAATCATTACTTCAAAACTAGGCATTATT8760.17765267280068628No Hit
ATGCGGTGGGTGTGCGAATCCACACCCCGAAGGACACCAAATACTCAAGA8660.17562467425273323No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC8380.16994627831846473No Hit
GGTTAGGAAAAAATCTAGCGGGCATAATTAACTTTAATCTCCTTTTTTCA8340.1691350788992835No Hit
ATTGACAACAGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT8070.1636594828198103No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCTTTTCCTGAT7840.15899508615951832No Hit
GCTTAGCGTGTATACATGCATACAAAGGCAATAAGGCAGGATAACCACAT7640.15493908906361223No Hit
GATTAACTGTATGTTCAGGATAATAAGGTTTAATACCTTTATCTAATGGC7580.1537222899348404No Hit
TATTAATAGATGTCAACAATTTGTGGGCCCTCTAACTACAAATGAAAAAA7550.1531138903704545No Hit
GTACCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG7540.1529110905156592No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT7510.15230269095127327No Hit
GATATACATTTGCAGGAGGACATTATTAATAGATGTCAACAATTTGTGGG7380.14966629283893432No Hit
AGATAAAGGTATTAAACCTTATTATCCTGAACATACAGTTAATCATTACT7370.14946349298413902No Hit
GGAACATATTGTACTAAAAATCAAGCAGTGTTTTCGAAAACTTCCTGTAA7300.1480438940005719No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT7220.14642149516220945No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7210.14621869530741416No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTGTTACCAATTTTCTTTTATC7210.14621869530741416No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG7060.14317669748548462No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG7050.1429738976306893No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG7040.142771097775894No Hit
GTTTTGAAGTAATGATTAACTGTATGTTCAGGATAATAAGGTTTAATACC6940.14074309922794095No Hit
TTGACATACTTTCCAATCAATAGGCCTATTTACAGGAAGTTTTCGAAAAC6920.14033749951835034No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC6870.13932350024437382No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC6830.1385123008251926No Hit
ACATAGAGGTGCCTTGAGCAGGAGTCGTGCAGGTCTTGCATGGCCCCGTG6800.1379039012608067No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC6800.1379039012608067No Hit
CTTTTACACAATGTGGTTATCCTGCCTTATTGCCTTTGTATGCATGTATA6800.1379039012608067No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG6800.1379039012608067No Hit
ATGTAAGACCTTGGGCAAGACCTGGTTGGCGTTCACGGTGGTCTCCATGC6770.1372955016964208No Hit
TAGTTAGAGGGCCCACAAATTGTTGACATCTATTAATAATGTCCTCCTGC6750.13688990198683018No Hit
CAATAGGCCTATTTACAGGAAGTTTTCGAAAACACTGCTTGATTTTTAGT6610.1340507040196959No Hit
TAATAAAACCAAGCGTTGGGGCTACTCCCTAAACTTCATGGGATATGTAA6530.1324283051813335No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG6520.1322255053265382No Hit
CTGTATGTTCAGGATAATAAGGTTTAATACCTTTATCTAATGGCAAATAT6510.13202270547174288No Hit
ATTAATAGATGTCAACAATTTGTGGGCCCTCTAACTACAAATGAAAAAAG6490.13161710576215227No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT6320.1281695082306321No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTTAGGGAG6310.1279667083758368No Hit
GGGTAAAGGGTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6240.12654710939226965No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA6210.12593870982788377No Hit
ACCAAGCTATGTTGCCCGTTTGTCCTCTAATTCCAGGAACATCAACAACA6140.12451911084431665No Hit
ATGTTAATATGGGCCTAAAGATCAGACAACTATTGTGGTTTCACATTTCT6140.12451911084431665No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT6130.12431631098952134No Hit
GGTTTAATACCTTTATCTAATGGCAAATATTTGGTAAGGTTAGGAAAAAA6110.12391071127993071No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA5990.12147711302238706No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCT5820.1180295154908669No Hit
CATTATTAATAGATGTCAACAATTTGTGGGCCCTCTAACTACAAATGAAA5700.11559591723332326No Hit
GTACAATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCC5690.11539311737852795No Hit
GTCTTTAAACACACAGTCTTTGAAGTACGCCTCAAGGTCGGTCGTTGACA5680.11519031752373265No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5670.11498751766893735No Hit
ATCTTTGGGTATCCATTTGAACCCTAATAAAACCAAGCGTTGGGGCTACT5610.11377071854016553No Hit
ATCCTGAACATACAGTTAATCATTACTTCAAAACTAGGCATTATTTGCAT5570.1129595191209843No Hit
CTCAAGAACCGTTTCTCTTCCAAAAGTAAGACAAGAAATGTGAAACCACA5420.10991752129905474No Hit
CCTTACCACAGGAACATATTGTACTAAAAATCAAGCAGTGTTTTCGAAAA5400.10951192158946414No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCAAAGGGTATGCAAA5360.10870072217028294No Hit
CTTTTTACCTCTGTTACCAATTTTCTTTTATCTTTGGGTATCCATTTGAA5350.10849792231548763No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5330.108092322605897No Hit
CCCTTTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACACGCAGCGCC5320.10788952275110171No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC5310.1076867228963064No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5290.1072811231867158No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTTCATCAGGCTCTGCTAG5290.1072811231867158No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCATCTCG5260.10667272362232989No Hit
GGCAATAAGGCAGGATAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAA5260.10667272362232989No Hit
ATGATTAACTGTATGTTCAGGATAATAAGGTTTAATACCTTTATCTAATG5230.10606432405794398No Hit
ATTTACAGGAAGTTTTCGAAAACACTGCTTGATTTTTAGTACAATATGTT5220.10586152420314868No Hit
TTCCTAACCTTACCAAATATTTGCCATTAGATAAAGGTATTAAACCTTAT5220.10586152420314868No Hit
GCTCTGTACCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA5190.10525312463876275No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5180.10505032478396745No Hit
ATATTGTACTAAAAATCAAGCAGTGTTTTCGAAAACTTCCTGTAAATAGG5130.10403632550999094No Hit
ATACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAG5120.10383352565519563No Hit
AGTTTAGGGAGTAGCCCCAACGCTTGGTTTTATTAGGGTTCAAATGGATA5080.1030223262360144No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTGT5080.1030223262360144No Hit
ATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG5040.1022111268168332No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGCCTAT4950.10038592812367546No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)