FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489844

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489844
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences667659
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC85651.2828404919277656No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT42950.6432924591745187No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG36760.5505804609838256No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA29770.44588629824506226No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28950.43360457958329024No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC28830.4318072549010798No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCGTAT25580.38312971142454455No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT23650.3542227394523252No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTAT19060.2854750703577724No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC18840.28217997510705317No Hit
TTATTAATAGATGTCAACAATACGTGGGCCCTCTTACAATTAATGAAAAA18220.27289379758229876No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA18200.27259424346859695No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA18190.2724444664117461No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT15990.23949351390455306No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC15300.22915889698184255No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA15190.22751134935648287No Hit
GTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGTACAATA15180.227361572299632No Hit
CTCTTACAATTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG14950.2239166999920618No Hit
GGATAATACGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT14160.2120843125008425No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATACGTGGGCCCTCT13680.20489501377200037No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC13570.20324746614664072No Hit
GTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT13550.20294791203293894No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAA13530.2026483579192372No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGGCCCACAATTCTTTGACATACT13470.20174969557813197No Hit
ATACTATATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA13420.20100081029387756No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA13240.19830482327056176No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA12790.19156485571227228No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG12660.18961775397321087No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT12620.18901864574580737No Hit
GGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGTACAAT12610.1888688686889565No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAA12580.18841953751840385No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA11690.17508937945867575No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC11680.17493960240182488No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA11280.1689485201277898No Hit
GTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCC10800.16175922139894766No Hit
TTGACATACTTTCCAATCGATAGGTCTATTTACAGGCAATTTTCGAAAAC10620.15906323437563188No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA10420.15606769323861433No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACACAGTCTTTGA10290.1541205914995529No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC10160.15217348976049153No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT10110.15142460447623712No Hit
TTATTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA9990.14962727979402657No Hit
CCCTTAACTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG9940.14887839450977222No Hit
GCGGTAGGAGTGCGAATCCACACTCCAAAAGATACCAAATACTCAAGAAC9240.13839400053021078No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG9230.1382442234733599No Hit
GTTCAGGATAATACGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTA9170.13734556113225463No Hit
ATATAGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTGTTTACC9140.136896229961702No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC9100.13629712173429848No Hit
CTTACAATTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT9090.1361473446774476No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG9060.13569801350689498No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA9040.13539845939319325No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCATCCACCAATCGGCAGT8730.13075537063081602No Hit
CTATATAAGAGAGAAACTACACGTAGCGCCTCATTTTGTGGGTCACCATA8650.129557154176009No Hit
AAAACTCAGACAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAA8640.12940737711915812No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG8320.12461451129993006No Hit
TTGTAAGAGGGCCCACGTATTGTTGACATCTATTAATAATGTCCTCCTGT8260.12371584895882479No Hit
GTTTTAGTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACA8250.12356607190197391No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA8150.12206830133346513No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG8110.12146919310606163No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTG8050.12057053076495637No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG7830.11727543551423707No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7770.11637677317313179No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC7600.11383056320666687No Hit
CTCCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG7570.11338123203611425No Hit
TCCTCACATTCATTTACAGGAGGACATTATTAATAGATGTCAACAATACG7560.11323145497926336No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT7430.11128435324020197No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC7290.10918747444428967No Hit
GATATAGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTAC7250.10858836621688615No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA7170.10739014976207915No Hit
ACGTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTA7160.10724037270522828No Hit
CAACAATACGTGGGCCCTCTTACAATTAATGAAAAAAGGAGATTAAAATT7070.10589237919357036No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATAC7070.10589237919357036No Hit
CAATTACATATCCCATAAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTA6910.10349594628395634No Hit
CTTTCCAATCGATAGGTCTATTTACAGGCAATTTTCGAAAACATTGCTTG6840.10244750688600018No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAT6770.10139906748804403No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC6740.10094973631749142No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT6700.1003506280900879No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA6700.1003506280900879No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA6680.10005107397638616No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)