FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences667659
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC82581.236858935474546No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG41760.6254689894092643No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT40840.6116895001789835No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28750.43060903844627274No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC28470.42641528085444813No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT25090.37579063563885157No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA24340.3645573563750358No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCGTAT23540.35257519182696556No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA20490.3068931894874479No Hit
TTATTAATAGATGTCAACAATACGTGGGCCCTCTTACAATTAATGAAAAA17470.2616605183184829No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC16620.24892946848615838No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTAT16510.2472819208607987No Hit
GTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGTACAATA16480.24683258969024607No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT16010.2397930680182548No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA15200.22766112641333375No Hit
CTCTTACAATTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG14740.2207713817981934No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA14490.21702695537692143No Hit
GTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT14470.21672740126321968No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGGCCCACAATTCTTTGACATACT14380.2153794077515618No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC14300.21418119129675478No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC14110.2113354272165881No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA14020.2099874337049302No Hit
GGATAATACGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT13800.20669233845421092No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG13470.20174969557813197No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA13330.19965281678221966No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAA13130.1966572756452021No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAA13090.19605816741779858No Hit
GGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGTACAAT12790.19156485571227228No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA11980.1794329141073512No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATACGTGGGCCCTCT11900.17823469765254418No Hit
ATACTATATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA11650.17449027123127225No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA11590.17359160889016698No Hit
GTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCC10950.1640058772517108No Hit
GTTCAGGATAATACGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTA10810.16190899845579854No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC10650.1595125655461845No Hit
CCCTTAACTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG10270.15382103738585115No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC10240.15337170621529853No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT10060.15067571919198272No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA9790.14663173865700904No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA9540.14288731223573709No Hit
TTATTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA9290.13914288581446516No Hit
TTGACATACTTTCCAATCGATAGGTCTATTTACAGGCAATTTTCGAAAAC9210.13794466935965816No Hit
GCGGTAGGAGTGCGAATCCACACTCCAAAAGATACCAAATACTCAAGAAC8560.12820916066435112No Hit
GTTTTAGTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACA8560.12820916066435112No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG8490.12716072126639497No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8390.1256629506978862No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG8300.1243149571862283No Hit
ATATAGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTGTTTACC8190.12266740956086863No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTG8120.12161897016291251No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA8080.121019861935509No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC8060.12072030782180725No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT7900.1183238749121932No Hit
CTTACAATTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT7690.11517855671832476No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG7690.11517855671832476No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA7600.11383056320666687No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7560.11323145497926336No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC7540.11293190086556161No Hit
CTCCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG7540.11293190086556161No Hit
TTGTAAGAGGGCCCACGTATTGTTGACATCTATTAATAATGTCCTCCTGT7330.10978658267169318No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATAC7320.1096368056148423No Hit
TCCTCACATTCATTTACAGGAGGACATTATTAATAGATGTCAACAATACG7320.1096368056148423No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATACGGTTTAATGCC7210.10798925798948267No Hit
AAAACTCAGACAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAA7160.10724037270522828No Hit
CTATATAAGAGAGAAACTACACGTAGCGCCTCATTTTGTGGGTCACCATA7160.10724037270522828No Hit
GTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAAC7080.10604215625042125No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCATCCACCAATCGGCAGT7060.10574260213671949No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA6990.10469416273876336No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAT6930.10379550039765809No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA6900.10334616922710546No Hit
CTTTCCAATCGATAGGTCTATTTACAGGCAATTTTCGAAAACATTGCTTG6860.10274706099970195No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6840.10244750688600018No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT6830.10229772982914931No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6820.10214795277229843No Hit
ACGTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTA6770.10139906748804403No Hit
GATATAGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTAC6720.10065018220378967No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC6720.10065018220378967No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)