FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences527019
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27330.5185771290978124No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG20700.39277521303786006No Hit
ATCCTAACCTTACCAAATATCTGCCCTTGGACAAAGGCATTAAACCATAT19860.3768365087406716No Hit
CTTATATAGAATGCCCGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT19000.3605183114840262No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAATTTCAT16120.30587132532223693No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG12510.23737284614027196No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA12040.22845476159303554No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA11030.20929036714046365No Hit
CTCTTATATAGAATGCCCGCCTTCCACAGAGTATGTAAATAATGCCTAGT10780.20454670514725276No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA10450.1982850713162144No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT10180.19316191636354665No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA9730.1846233247757671No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA9260.17570524022853068No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT8870.16830512751912172No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACCTACAAGGCA8810.1671666486407511No Hit
GGATAATATGGTTTAATGCCTTTGTCCAAGGGCAGATATTTGGTAAGGTT8770.16640766272183735No Hit
GTTCTATCCTAACCTTACCAAATATCTGCCCTTGGACAAAGGCATTAAAC8750.1660281697623805No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT8620.16356146552591083No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG8420.15976653593134213No Hit
ATATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT8170.15502287393813124No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT8150.1546433809786744No Hit
CCCTTAATTTCATGGGATATGTAATTGGATGTTGGGGTACTTTACCGCAA8150.1546433809786744No Hit
GCATCAAACCTCGACAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCT8070.15312540914084694No Hit
ATGTAATTGGATGTTGGGGTACTTTACCGCAAGAACATATTGTACTCAAA8020.15217667674220475No Hit
CAATATGTTCTTGCGGTAAAGTACCCCAACATCCAATTACATATCCCATG7690.1459150429111664No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGCGAGTCCAA7650.14515605699225265No Hit
CATATAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAATTTCAT7480.14193036683686927No Hit
AACATATTGTACTCAAAATCAAACAATGTTTTCGAAAACTGCCTGTAAAT7370.13984315555985646No Hit
GTTTGATGCTGTAGCTCTTGTTCCCAAGAATATGGTGACCCACAAAATGA7310.13870467668148587No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT7210.1368072118842015No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAATGCCTTGTAGGTTGGCGA7050.13377126820854657No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6850.12997633861397787No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA6800.12902760621533568No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC6780.12864811325587883No Hit
GTTTGGAAGACCAACCTCCCATGCTGTAGCTCTTGTTCCCAAGAATATGG6430.1220069864653836No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGTTTGATTTTGAGTACAATA6320.11991977518837083No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC6310.11973002870864237No Hit
ATTTACATACTCTGTGGAAGGCGGGCATTCTATATAAGAGAGAAACTACA6260.1187812963100002No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6200.1176428174316296No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC6190.11745307095190118No Hit
GGTCTATTTACAGGCAGTTTTCGAAAACATTGTTTGATTTTGAGTACAAT6180.11726332447217272No Hit
AATTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA6170.11707357799244429No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAAC6120.11612484559380212No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC6080.11536585967488837No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA6070.11517611319515994No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT6050.11479662023570307No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6000.1138478878370609No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT5980.11346839487760405No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCTCCGTG5940.11270940895869029No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA5930.11251966247896186No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG5830.11062219768167751No Hit
CAATTACATATCCCATGAAATTAAGGGAGTAGCCCCAACGTTTGGTTTTA5750.10910422584385004No Hit
GCATGGGAGGTTGGTCTTCCAAACCTCGACAAGGCATGGGGACGAATCTT5720.10853498640466473No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT5700.10815549344520786No Hit
GTCTTACATAAGAGGACTCTTGGACTCGCAGCAATGTCAACGACCGACCT5660.10739650752629411No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5640.10701701456683724No Hit
GTTCAGGATAATATGGTTTAATGCCTTTGTCCAAGGGCAGATATTTGGTA5570.10568878920873821No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5400.10246309905335481No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5380.10208360609389794No Hit
CAATAGGTCTATTTACAGGCAGTTTTCGAAAACATTGTTTGATTTTGAGT5330.10113487369525576No Hit
GTATAGGCCCCTCACTCTGGGGTCTAGCAGAGCTTGGTGGAATGTTGTGG5330.10113487369525576No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)