FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489848

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489848
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences535397
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG36550.6826709899383074No Hit
CTCTTACAGTTAACGAAAAAAGGAGACTAAAATTGATTATGCCTGCTAGG24620.4598456846041349No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG21090.39391330171816424No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCACTTAACTTCAT20420.3813992233800339No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT19650.36701737215561536No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18170.3393743334385512No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT17970.335638787665975No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT17020.31789494524623785No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGACTAAAATTGA16820.31415939947366156No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT14850.2773642736137857No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG14660.2738155051298382No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGACTAAAATTGATTATG14590.27250806410943657No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT13580.25364355795792654No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA13090.24449147081511477No Hit
TATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAA12890.24075592504253854No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT12540.23421871994053012No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT12280.229362510436181No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA11640.21740876396393705No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCA11640.21740876396393705No Hit
GGTTAGGATAAAACCTAGCAGGCATAATCAATTTTAGTCTCCTTTTTTCG11560.21591454565490653No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT11270.210498004284671No Hit
CAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGA11050.20638890393483716No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC9750.18210785641309157No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAAT9480.17706486962011367No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC9350.1746367648679391No Hit
ATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAA9320.1740764330020527No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC9160.1710879963839917No Hit
CAATATGTTCTTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATG9070.1694070007863324No Hit
GATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC8790.16417723670472564No Hit
CTCCAAAAGACACCAAATACTCGAGAACAGTTTCTCTTCCAAAAGTAAGA8780.16399045941609686No Hit
ATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGACTAAAATTGATTA8730.16305657297295278No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC8690.16230946381843753No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC8540.15950780448900534No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC8530.15932102720037655No Hit
CAATAGGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGT8450.15782680889134604No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA8310.1552119268505427No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC8110.15147638107796646No Hit
GTTTGGAAGTAATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCC7980.1490482763257919No Hit
GGATAAAACCTAGCAGGCATAATCAATTTTAGTCTCCTTTTTTCGTTAAC7920.14792761259401901No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG7700.14381851224418515No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG7590.14176396206926822No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG7550.141016852914753No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7520.14045652104886652No Hit
ATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGA7450.13914908002846488No Hit
CACTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAA7410.13840197087394962No Hit
ATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGG7260.13560031154451743No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC7110.13279865221508524No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG6860.12812921999936494No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA6860.12812921999936494No Hit
CAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGACTAAAATT6580.12289945591775822No Hit
TTATTATCCTGAACACATAGTTAATCATTACTTCCAAACTAGGCATTATT6510.12159201489735653No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6490.12121846032009892No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT6470.12084490574284128No Hit
GATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGACTA6390.1193506874338108No Hit
ATATGTTCTTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA6360.11879035556792436No Hit
GTACAATATGTTCTTGTGGTAAGGTACCCCAACTTCCAATTACATATCCC6350.11860357827929555No Hit
CTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTG6330.11823002370203793No Hit
ATCTAAGGGCAAATATTTGGTAAGGTTAGGATAAAACCTAGCAGGCATAA6270.11710935997026506No Hit
GGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATG6270.11710935997026506No Hit
ATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAG6220.11617547352712099No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC6190.11561514166123456No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6160.11505480979534811No Hit
GTGTACAATATGTTCTTGTGGTAAGGTACCCCAACTTCCAATTACATATC6160.11505480979534811No Hit
CTTACAGTTAACGAAAAAAGGAGACTAAAATTGATTATGCCTGCTAGGTT6150.11486803250671933No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT6140.1146812552180905No Hit
ATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAG6040.11281348233180238No Hit
ACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATGTGAGG6030.11262670504317357No Hit
GCCTTTATCTAAGGGCAAATATTTGGTAAGGTTAGGATAAAACCTAGCAG6020.11243992775454477No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCT5710.10664983180705158No Hit
ATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGGAT5650.1055291680752787No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5640.10534239078664992No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCT5450.10179362230270249No Hit
GTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTTAGGATAAAAC5410.10104651314818723No Hit
ATCCTGAACACATAGTTAATCATTACTTCCAAACTAGGCATTATTTACAT5410.10104651314818723No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5410.10104651314818723No Hit
GCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAAC5360.10011262670504316No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)