FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences535397
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG45630.8522647680132686No Hit
CTCTTACAGTTAACGAAAAAAGGAGACTAAAATTGATTATGCCTGCTAGG21670.4047463844586353No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG20170.3767297911643136No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT18610.34759253413821894No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT17960.3354520103773462No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17940.33507845580008855No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCACTTAACTTCAT17670.3300354690071106No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT16910.31584039507132095No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGACTAAAATTGA14240.2659708590074281No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG13630.2545774444010706No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT13500.2521493396488961No Hit
GGTTAGGATAAAACCTAGCAGGCATAATCAATTTTAGTCTCCTTTTTTCG13280.2480402392990622No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT13040.2435575843719707No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCA12740.23795426571310638No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGACTAAAATTGATTATG12680.2368336019813335No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA12550.23440549722915893No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT12250.22880217857029458No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT12090.22581374195223355No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA11950.22319885991143024No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT11910.22245175075691495No Hit
TATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAA11560.21591454565490653No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAAT11470.21423355005724723No Hit
CAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGA9990.1865905113401831No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC9690.18098719268131871No Hit
GTTTGGAAGTAATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCC9690.18098719268131871No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC9510.1776252014860001No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC9360.17482354215656795No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA9360.17482354215656795No Hit
GATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC9340.1744499875793103No Hit
ATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAA8940.16697889603415783No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC8650.1615623546639223No Hit
CAATATGTTCTTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATG8530.15932102720037655No Hit
GGATAAAACCTAGCAGGCATAATCAATTTTAGTCTCCTTTTTTCGTTAAC8250.1540912631187698No Hit
CTCCAAAAGACACCAAATACTCGAGAACAGTTTCTCTTCCAAAAGTAAGA8120.15166315836659527No Hit
ATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGACTAAAATTGATTA7960.14867472174853427No Hit
CAATAGGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGT7780.14531273055321564No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC7550.141016852914753No Hit
ATCTAAGGGCAAATATTTGGTAAGGTTAGGATAAAACCTAGCAGGCATAA7410.13840197087394962No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC7370.13765486171943436No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC7320.13672097527629032No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG7290.13616064341040388No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7220.13485320239000217No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7220.13485320239000217No Hit
GTGTACAATATGTTCTTGTGGTAAGGTACCCCAACTTCCAATTACATATC6960.12999699288565308No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC6880.1285027745766226No Hit
ATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGA6870.12831599728799378No Hit
CTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTG6870.12831599728799378No Hit
ATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGGAT6680.12476722880404635No Hit
TTATTATCCTGAACACATAGTTAATCATTACTTCCAAACTAGGCATTATT6640.1240201196495311No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA6640.1240201196495311No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG6600.12327301049501584No Hit
GTACAATATGTTCTTGTGGTAAGGTACCCCAACTTCCAATTACATATCCC6520.12177879218598535No Hit
ATATGTTCTTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA6500.12140523760872773No Hit
GTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTTAGGATAAAAC6440.12028457387695485No Hit
CACTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAA6440.12028457387695485No Hit
ATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGG6390.1193506874338108No Hit
GTTTCTCTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATG6280.11729613725889386No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG6250.11673580539300743No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6080.11356059148631764No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT6070.11337381419768881No Hit
GGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATG6050.1130002596204312No Hit
ACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATGTGAGG6000.11206637317728713No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC5900.11019860029099901No Hit
ATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAG5890.11001182300237021No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5880.1098250457137414No Hit
GATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGACTA5880.1098250457137414No Hit
CTTCAAAGACTGTGTGTTTAACGACTGGGAGGAGTTGGGGGAGGAGATTA5830.10889115927059734No Hit
GCCTTTATCTAAGGGCAAATATTTGGTAAGGTTAGGATAAAACCTAGCAG5820.10870438198196852No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTA5800.10833082740471091No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5790.1081440501160821No Hit
GGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTTAGGATAAAA5590.10440850434350585No Hit
CTTACAGTTAACGAAAAAAGGAGACTAAAATTGATTATGCCTGCTAGGTT5540.10347461790036179No Hit
ATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAG5420.10123329043681606No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCT5410.10104651314818723No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)