FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences487562
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN43220.8864513641342023No Hit
ATATCGTTTTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC31470.6454563727279813No Hit
ATATGGTTTTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC20800.42661241031909786No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCGGAAAGTCCAA20570.4218950615511463No Hit
AAACCATATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA18800.38559198624995383No Hit
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGTAA17230.3533909533556758No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA16110.33041951587695517No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG14590.2992439935844057No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATGACATATCCCATGAA14320.2937062363350712No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT13900.285091947280551No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12970.266017450088399No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT12670.2598643864780274No Hit
CTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT12510.25658275255249585No Hit
CTCTTGGACTTTCCGCAATGTCAACGACCGACCTTGAGGCATACTTCAAA11220.23012457902789799No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGAAAACTTCCTGTAA10830.2221255963344149No Hit
CATGTATACAAGCCAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC10610.21761334968680907No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT10540.21617763484438904No Hit
AAACGATATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA10140.20797355003056023No Hit
TGATTAACTACATGCTCTGGATAATACGGTTTGATCCCTTTATCTAAGGG10020.20551232458641158No Hit
CAATAGGCCTGTTTACAGGAAGTTTTCTAAAACACATTTTGATTTTTTGT9990.2048970182253744No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCCAAACAG9950.20407660974399153No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT9820.20141028217949716No Hit
GTTTACAGGAAGTTTTCTAAAACACATTTTGATTTTTTGTACAATATGTT9220.18910415495875396No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC9150.18766844011633393No Hit
GACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAG9050.18561741891287672No Hit
CCCAATGACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT8960.18377149982976526No Hit
GTTTCGCCCCAGACCTGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGCA8860.18172047862630805No Hit
ATATAGTTTTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC8680.17802864046008507No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT8560.17556741501593642No Hit
GCACAACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACA8490.1741317001735164No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAGGTTCCCGTGGGCGTTC8250.16920924928521913No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG8130.16674802384107046No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA7930.16264598143415607No Hit
GACCTTCAACCTAACCTCCGCCCCCAACTCCTCCCACTCAGTAAACACAC7790.15977455174931599No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC7460.15300618177790723No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA7390.15157046693548717No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGCGGAGGTTA7290.14951944573202997No Hit
ATCTTAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG7250.1486990372506471No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA7160.1468531181675356No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7050.1445969948437327No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA6930.14213576939958406No Hit
GTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATAC6800.1394694418350897No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT6660.1365980121502496No Hit
GTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTTTG6640.13618780790955817No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG6620.13577760366886674No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG6520.13372658246540953No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC6430.13188066338229804No Hit
ATGTCATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA6240.12798372309572936No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCCAAACAGGCTTTTACTTT6230.12777862097538364No Hit
ATATTGTTTTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC6190.12695821249400074No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC6180.12675311037365505No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCCAAAC6100.12511229341088928No Hit
AGTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATA6030.12367657856846924No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT6030.12367657856846924No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG5950.12203576160570348No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT5800.11895922980051767No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT5770.11834392343948051No Hit
GTAATGTTTAACCTAACCTCCGCCCCCAACTCCTCCCACTCAGTAAACAC5750.11793371919878907No Hit
CAATGACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG5740.11772861707844336No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5720.11731841283775192No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC5660.1160878001156776No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT5630.11547249375464044No Hit
GGGCAAAACTCATCGGGACTGACAATTCTGTCGTGCTCTCCCGCAAGTAT5600.11485718739360329No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA5490.11260106406980036No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT5470.11219085982910892No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5390.11055004286634315No Hit
GTCATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAAAT5310.1089092259035774No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTA5270.10808881742219452No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG5250.10767861318150306No Hit
GAACCTGCACCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATAC5240.10747351106115735No Hit
GGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCTTTTGGGGTTT5170.10603779621873731No Hit
TCCTAGTACAAAGTAATGTTTAACCTAACCTCCGCCCCCAACTCCTCCCA5030.10316636653389723No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC5020.10296126441355152No Hit
GTACAATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATGACATATCCC5010.10275616229320579No Hit
AAGTCAACAGTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTC5000.10255106017286006No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG4990.10234595805251434No Hit
CCCTTAACTTCATGGGATATGTCATTGGGAGTTGGGGCACATTGCCACAG4980.10214085593216864No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG4890.10029493684905716No Hit
GTCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATT4880.10008983472871143No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)