FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489852

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489852
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences512748
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN38960.7598274395999595No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT18510.3609960448407405No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG16830.32823141192164573No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT16470.3212104191532683No Hit
GAAATAAGGAGATTAAAATTGATTATGCCTGCTAGGTTCTATCCTAACCG12250.23890878170173263No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT12130.23656845077894015No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT11830.23071762347195895No Hit
ATCCTAACCGTACCAAGTATTTGCCCTTAGATAAAGGCATTAAACCTTAT10710.20887453485922908No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT9990.1948325493224742No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATACTTGGTACGGTT9380.18293586713161242No Hit
CATGTATACAAGCTAAACAAGCTTTCACTTTCTCGCCAACTTACAAGACT9240.18020548105502118No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCGCTTACAGTGAACG8490.16557841278756816No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA8350.16284802671097692No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8260.16109277851888257No Hit
CCTTCTTTCGTTCACTGTAAGCGGGCCTACATATTGTTGACATCTATTGA8230.16050769578818447No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG8220.16031266821128506No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG8190.15972758548058696No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA8130.1585574200191907No Hit
GTTCTATCCTAACCGTACCAAGTATTTGCCCTTAGATAAAGGCATTAAAC7740.15095134452011513No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA7710.15036626178941703No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT7560.14744084813592642No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAAT7560.14744084813592642No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAACAAGCTTTCACTTT7220.14080991052134773No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC7080.1380795244447565No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAAGTCTTGTAAGTTGGCGA7060.13768946929095774No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6840.13339886259917152No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6700.1306684765225803No Hit
CGAAATAAGGAGATTAAAATTGATTATGCCTGCTAGGTTCTATCCTAACC6560.12793809044598906No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC6520.12715798013839158No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAAATGTATA6400.12481764921559908No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6400.12481764921559908No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG6330.12345245617730347No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCA6300.12286737344660535No Hit
ATGTAAGACCTTGGGCAAGACCTGTCGGGCGTTCACGGTGGTCTCCATGC6290.12267234586970598No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6130.119551904639316No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC6110.11916184948551724No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6070.11838173917791976No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG6040.11779665644722162No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA5820.11350604975543543No Hit
CAATAGGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGT5680.1107756636788442No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5640.1099955533712467No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC5580.10882538790985044No Hit
GGAACATATTGTACACAAAATCAAACAATGTTTTCGGAAACTTCCTATAA5470.10668008456395735No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC5400.10531489152566173No Hit
GTCTTACATAATAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5370.10472980879496362No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATACTTGGTA5350.10433975364116485No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCGTCCAAAAGTAAGA5350.10433975364116485No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCT5330.10394969848736611No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG5160.10063422968007676No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT5160.10063422968007676No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)