FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences512748
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN38770.7561219156388713No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG22940.44739326140716296No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT16480.32140544673016763No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT14480.2823999313502929No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT12070.2353982853175439No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT10570.20614414878263784No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT10370.20224359724465038No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATACTTGGTACGGTT9870.1924922183996817No Hit
GAAATAAGGAGATTAAAATTGATTATGCCTGCTAGGTTCTATCCTAACCG9750.19015188747688924No Hit
ATCCTAACCGTACCAAGTATTTGCCCTTAGATAAAGGCATTAAACCTTAT9440.18410603259300864No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA8660.1688938815948575No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8650.16869885401795814No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA8460.16499333005687006No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCGCTTACAGTGAACG8450.16479830247997068No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG8340.16265299913407755No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA8290.16167786124958067No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAAGTCTTGTAAGTTGGCGA8260.16109277851888257No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG8090.15777730971159323No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAAT8070.15738725455779445No Hit
GTTCTATCCTAACCGTACCAAGTATTTGCCCTTAGATAAAGGCATTAAAC8030.15660714425019698No Hit
CCTTCTTTCGTTCACTGTAAGCGGGCCTACATATTGTTGACATCTATTGA8020.1564121166732976No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC7690.14997620663561828No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT7620.14861101359732265No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG7270.14178504840584458No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT7050.13749444171405836No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCA6950.13554416594506463No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC6880.13417897290676903No Hit
CATGTATACAAGCTAAACAAGCTTTCACTTTCTCGCCAACTTACAAGACT6760.13183864198397655No Hit
GTCTTACATAATAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6530.12735300771529096No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATACTTGGTA6430.12540273194629722No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAACAAGCTTTCACTTT6310.12306240102350473No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA6230.12150218040830973No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC6110.11916184948551724No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6100.11896682190861788No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC5820.11350604975543543No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5790.11292096702473729No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5700.11116571883264294No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5620.10960549821744794No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAAATGTATA5550.10824030517915234No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5510.10746019487155482No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG5480.10687511214085672No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA5450.10629002941015861No Hit
CAATAGGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGT5410.1055099191025611No Hit
GTTTGGAAGTAATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCC5360.10453478121806423No Hit
GTTTTAGTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAAC5300.103364615756668No Hit
GTACAATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCC5230.10199942271837238No Hit
GATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC5160.10063422968007676No Hit
CTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTG5150.1004392021031774No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)