FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences629415
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG38910.6181930840542409No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN31260.49665165272514955No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG21720.3450823383618122No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT19080.3031386287266748No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA18070.28709198223747445No Hit
CATACTTTCCAATCAATAGGTCTATTTATAGGAAGTTTCCGAAAACATTG17520.27835370939682086No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG16220.25769960995527597No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGGACAATATGTT16210.25754073226726404No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTT15690.2492790924906461No Hit
CATTTGAACCCTACTAAAACTAAACGTTGGGGCTATTCCCTTCACTTCAT15170.24101745271402814No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT14960.23768102126577856No Hit
GGATATTATTAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG14590.23180254680933882No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGA14410.2289427484251249No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC13690.21750355488826928No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT13500.2144848788160435No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATG13300.2113073250558058No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA13160.20908303742363943No Hit
TTATTAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAA13000.20654099441544926No Hit
GTGTAAAGGGAGCGGCAAATCCCAGAAGCCCCACAATTCGTTGACATATT12950.20574660597538985No Hit
CATATTTTCCAATCAATAGGTCTATTTATAGGAAGTTTCCGAAAACATTG12770.20288680759117592No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG12040.19128873636630842No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT11980.1903354702382371No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT11650.18509250653384493No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA11470.182232708149631No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA11280.17921403207740522No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCA11240.17857852132535768No Hit
CATGTATACAAGCCAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC10900.17317667993295363No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGGACAAT10750.17079351461277537No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG10630.16888698235663274No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC10130.16094309795603856No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA9920.15760666650778898No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA9750.15490574581158695No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC9640.15315809124345622No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC9290.14759737216304028No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA9250.14696186141099274No Hit
TATTAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAA9120.14489645146683827No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC9000.14298991921069565No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG8880.14108338695455305No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA8780.1394946100744342No Hit
GTTTGGAAGTAATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCC8330.1323451141138994No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8270.1313918479858281No Hit
GTTTTAGTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAC8240.13091521492179245No Hit
CTATTAATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTG7870.12503674046535276No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7810.12408347433728144No Hit
GGGTAAAGGTTCAGATATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA7770.1234479635852339No Hit
ATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTA7740.12297133052119825No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT7610.12090592057704376No Hit
CAATAGGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGG7560.12011153213698435No Hit
GATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC7510.11931714369692492No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT7320.11629846762469913No Hit
CTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAGGAACATA7260.11534520149662782No Hit
TTATTATCCTGAACACATAGTTAATCATTACTTCCAAACTAGGCATTATT7110.11296203617644955No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7080.11248540311241391No Hit
ATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGA7020.11153213698434261No Hit
GGAACATATTGTCCACAAAATCAAACAATGTTTTCGGAAACTTCCTATAA6940.11026111548024754No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTAATAATATCCTCTTTC6830.10851346091211679No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTGACTGGCAAACTCCCTCTTTTC6720.10676580634398608No Hit
ATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCCAGTC6710.10660692865597421No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6700.10644805096796232No Hit
GAAAATATGTCAACGAATTGTGGGGCTTCTGGGATTTGCCGCTCCCTTTA6690.10628917327995042No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT6670.10597141790392667No Hit
ATGTAATTGGAAGTTGGGGTACCTTACCACAGGAACATATTGTCCACAAA6580.10454151871181971No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGCGT6440.10231723107965332No Hit
CTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTG6410.10184059801561768No Hit
GTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATTGTG6380.10136396495158202No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)