FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489856

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489856
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239672
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA7840.327113722086852No Hit
CTCATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6920.28872792816849696No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT6560.27370740011348843No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC6360.2653626623051504No Hit
GAAAACAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTATCCCAATGT5940.24783871290764042No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA5900.24616976534597282No Hit
CCTCCTGCATATAGACCTCCAAATGCCCCTATCTTATCAACACTTCCGGA5570.23240094796221503No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT5250.21904936746887416No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4860.20277712874261491No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC4820.2011081811809473No Hit
GGAACATATTGTACACAAAATCAAAACGTGTTTTAGGAAACTTCCTGTAA4740.19777028605761207No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT4680.19526686471511065No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA4640.19359791715344304No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT4560.1902600220301078No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG4470.18650489001635567No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT4230.17649120464635001No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA4210.1756567308655162No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC4200.1752394939750993No Hit
GTTTACAGGAAGTTTCCTAAAACACGTTTTGATTTTGTGTACAATATGTT4070.16981541439967957No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT4020.16772922994759504No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACGTTTTGATTTTGTGT3990.16647751927634433No Hit
CCTATTTTCATTTACTGTAAGGGGCCCCACAAATTGCTTACATCTATCAA3980.16606028238592743No Hit
GCACCGGACCATGCAAAACCTGCACAACGCCTGCTCAAGGAACCTCTATG3900.16272238726259222No Hit
ATATGTTCCTGTGGCAAGGTGCCCCAACTCCCAATTACATATCCCATGAA3830.15980172902967388No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC3720.15521212323508796No Hit
ACCTAGCAGCCATGGAAACGATGTATACTTGCGGGAGAGCACGACAGAAT3600.1502052805500851No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA3600.1502052805500851No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG3460.14436396408424848No Hit
CTCTAAGGCCTCCCGATACAAAGCAGAGGCGGTGTCGAGGAGATCTCGAA3410.14227777963216395No Hit
CTTTTATATAAGATCCCCGCTTTCCAAAGAGTGTGTAAATAATGTCGCGT3360.14019159518007943No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA3340.13935712139924564No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT3330.13893988450882874No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA3250.1356019893854935No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT3220.1343502787142428No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT3190.1330985680429921No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT3190.1330985680429921No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA3180.1326813311525752No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA3170.1322640942621583No Hit
GCTTTAGGGCATGGACATTGACACCTATAAAGAATTTGGAGCTTCTGTGG3160.13184685737174137No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG3160.13184685737174137No Hit
CTTTAGGGCATGGACATTGACACCTATAAAGAATTTGGAGCTTCTGTGGA3150.13142962048132448No Hit
CCTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCT3080.12850896224840616No Hit
ACATAGAGGTTCCTTGAGCAGGCGTTGTGCAGGTTTTGCATGGTCCGGTG3060.12767448846757234No Hit
GTAGCTCTTGTTCCCAAGAATATGGTGACCCACAAAATGAGGCGCTACGT3030.12642277779632163No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC2990.12475383023465403No Hit
GTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTGAG2990.12475383023465403No Hit
CAGTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTG2920.1218331720017357No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC2900.1209986982209019No Hit
GTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATAC2870.1197469875496512No Hit
CAAGATCTACAGCATGGGAGGTTGGTCTTCCAAACCTCGAAAAGGCATGG2850.11891251376881738No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT2840.11849527687840047No Hit
CACACAATAGCTTGCCTGAGTGCCGTATGGTGAGGTGAACAATGTTCCGG2810.11724356620714978No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGTGGGTCACCATATT2780.11599185553589905No Hit
GTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGCAAGACCTTGGG2770.11557461864548217No Hit
ATGTAATTGGGAGTTGGGGCACCTTGCCACAGGAACATATTGTACACAAA2750.11474014486464836No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC2740.11432290797423145No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT2740.11432290797423145No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA2730.11390567108381455No Hit
GGCCGAGACAGTATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCAC2720.11348843419339764No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTGTTCG2720.11348843419339764No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA2680.11181948663173003No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG2560.1068126439467272No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACCTTGCCACAG2530.10556093327547648No Hit
ACACACAATAGCTTGCCTGAGTGCCGTATGGTGAGGTGAACAATGTTCCG2530.10556093327547648No Hit
ATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTTC2530.10556093327547648No Hit
CACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAACCT2520.10514369638505959No Hit
GCATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAAAACCACATC2520.10514369638505959No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT2500.10430922260422579No Hit
CTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT2490.10389198571380887No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA2490.10389198571380887No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA2480.10347474882339198No Hit
GGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA2480.10347474882339198No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACGCCCATAGGTAT2460.10264027504255815No Hit
TCCTAGGAGTCCTGATGCGATGTTCTCCATGTTCGGTACAGGGTCCCCAG2430.10138856437130746No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG2430.10138856437130746No Hit
TTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTCCTAAAAC2420.10097132748089055No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT2420.10097132748089055No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)