FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489857

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489857
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239672
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA8040.3354584598951901No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC6450.2691177943189025No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT6160.2570179244968123No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT5920.24700423912680666No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA5730.23907673820888548No Hit
GAAAACAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTATCCCAATGT5520.2303147635101305No Hit
CTCATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5120.2136252878934544No Hit
CCTCCTGCATATAGACCTCCAAATGCCCCTATCTTATCAACACTTCCGGA4920.2052805500851163No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC4820.2011081811809473No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4820.2011081811809473No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT4710.19651857538636133No Hit
GTTTACAGGAAGTTTCCTAAAACACGTTTTGATTTTGTGTACAATATGTT4540.18942554824927402No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACGTTTTGATTTTGTGT4270.17816015220801762No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA4260.17774291531760072No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC4250.17732567842718383No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG4210.1756567308655162No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA4140.17273607263259788No Hit
GGAACATATTGTACACAAAATCAAAACGTGTTTTAGGAAACTTCCTGTAA4030.16814646683801193No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA4020.16772922994759504No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT4020.16772922994759504No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC3960.16522580860509364No Hit
ATATGTTCCTGTGGCAAGGTGCCCCAACTCCCAATTACATATCCCATGAA3900.16272238726259222No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA3810.1589672552488401No Hit
GCACCGGACCATGCAAAACCTGCACAACGCCTGCTCAAGGAACCTCTATG3770.15729830768717248No Hit
GCTTTAGGGCATGGACATTGACACCTATAAAGAATTTGGAGCTTCTGTGG3690.15396041256383725No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA3680.15354317567342035No Hit
CTTTTATATAAGATCCCCGCTTTCCAAAGAGTGTGTAAATAATGTCGCGT3640.15187422811175272No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG3630.15145699122133582No Hit
CTCTAAGGCCTCCCGATACAAAGCAGAGGCGGTGTCGAGGAGATCTCGAA3550.14811909609800059No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT3450.14394672719383156No Hit
CCTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCT3430.14311225341299777No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT3300.137688173837578No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC3270.13643646316632732No Hit
CTTTAGGGCATGGACATTGACACCTATAAAGAATTTGGAGCTTCTGTGGA3160.13184685737174137No Hit
CCTATTTTCATTTACTGTAAGGGGCCCCACAAATTGCTTACATCTATCAA3150.13142962048132448No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT3140.13101238359090758No Hit
GTAGCTCTTGTTCCCAAGAATATGGTGACCCACAAAATGAGGCGCTACGT3140.13101238359090758No Hit
GTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTGAG3140.13101238359090758No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT3060.12767448846757234No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC3060.12767448846757234No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT3060.12767448846757234No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT3030.12642277779632163No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT3020.12600554090590474No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC2940.12266764578256953No Hit
ATGTAATTGGGAGTTGGGGCACCTTGCCACAGGAACATATTGTACACAAA2930.1222504088921526No Hit
CAGTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTG2930.1222504088921526No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA2920.1218331720017357No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA2820.11766080309756667No Hit
GTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATAT2800.11682632931673287No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGTGGGTCACCATATT2770.11557461864548217No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC2760.11515738175506525No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT2740.11432290797423145No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG2720.11348843419339764No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC2710.11307119730298075No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTGTTCG2700.11265396041256384No Hit
GGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA2690.11223672352214692No Hit
CTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTG2690.11223672352214692No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG2660.11098501285089622No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT2660.11098501285089622No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACCTTGCCACAG2630.10973330217964551No Hit
ACATAGAGGTTCCTTGAGCAGGCGTTGTGCAGGTTTTGCATGGTCCGGTG2610.10889882839881171No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA2560.1068126439467272No Hit
GGCCGAGACAGTATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCAC2530.10556093327547648No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG2510.10472645949464268No Hit
GGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCTTTTGGGGTTT2490.10389198571380887No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT2490.10389198571380887No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG2470.10305751193297506No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA2460.10264027504255815No Hit
CAAGATCTACAGCATGGGAGGTTGGTCTTCCAAACCTCGAAAAGGCATGG2400.10013685370005673No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)