FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489858

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489858
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences699051
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT34510.49366927448784137No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN32540.46548821187581446No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA28360.4056928607497879No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA26090.37322026576029504No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAGCATT24320.3479002247332455No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC23780.34017546645380664No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA21090.30169472613586135No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG20790.2974031937583953No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT18060.2583502491234545No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA17820.2549170232214817No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT17300.24747836710054058No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT16390.23446071888889364No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT16210.23188579946241406No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA16130.23074139082842313No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC15460.22115696851874897No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTATATATCCCATGAA15360.21972645772626032No Hit
ATATAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA15210.21758069153752732No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC14420.20627965627686676No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT14220.20341863469188942No Hit
CAATTATATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG14170.2027033792956451No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT14110.20184507282015188No Hit
GTTTTATCCCAATGCTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC13900.19884100015592565No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCAACCAATCGGCAGT13840.19798269368043248No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG13780.19712438720493924No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA13770.1969813361256904No Hit
ACTAAATATTTGCCCTTAGATAAAGGGATCAAACCGTATTATCCAGAGTA13690.19583692749169948No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG13290.19011488432174475No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA12870.18410673899329233No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA12730.18210402388380817No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT12340.17652503179310236No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG12010.17180434617788973No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA11890.17008773322690332No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA11550.16522399653244182No Hit
GTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGG10890.1557826253020166No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC10670.1526355015585415No Hit
ATTATATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTGTG10630.15206329724154602No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAATTGGCAAACTCCTT10530.15063278644905737No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10440.14934532673581755No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTA10420.14905922457731982No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC10280.1470565094678357No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG10170.14548294759609812No Hit
CTTTATCTAAGGGCAAATATTTAGTAGCATTGGGATAAAACCTAGCAGGC9930.1420497216941253No Hit
GGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGA9920.14190667061487644No Hit
CTTCAAAGACTGTGTGTTTAATGAGTGGGAGGAGTTGGGGGAGGAGGTTA9850.14090531306013437No Hit
CTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATAT9830.14061921090163665No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC9720.1390456490298991No Hit
ATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC9520.13618462744492177No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC9470.1354693720486774No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT9460.13532632096942857No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG9450.13518326989017967No Hit
TAGATAAAGGGATCAAACCGTATTATCCAGAGTATGTAGTTAATCATTAC9450.13518326989017967No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT9340.13360970801844216No Hit
ATATTTAGTAGCATTGGGATAAAACCTAGCAGGCATAATTAATTTTAGTC9100.13017648211646932No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA9070.1297473288787227No Hit
TTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTCCTAAAAC9040.1293181756409761No Hit
CTATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGT9030.12917512456172725No Hit
GAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACA9020.1290320734824784No Hit
GCTTTAATCCTAATTGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAG8960.12817376700698518No Hit
GTACAAGAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG8750.12516969434275896No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT8640.12359613247102143No Hit
GTTGTGAGTAGGCCCTGAGCGTGAGGGCTCCACCCCAACAGTCCCCCATG8610.12316697923327481No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG8510.12173646844078614No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC8490.1214503662822884No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT8430.1205920598067952No Hit
ATCTAAGGGCAAATATTTAGTAGCATTGGGATAAAACCTAGCAGGCATAA8430.1205920598067952No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT8400.12016290656904861No Hit
GGGTAAAGGTGCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA8290.11858934469731106No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA8150.11658662958782691No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC8150.11658662958782691No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAGTCTCCTGTTTTCATTTAC8120.11615747635008032No Hit
CACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTTGC7980.11415476124059618No Hit
TGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGA7950.11372560800284956No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT7920.11329645476510297No Hit
CAGTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTG7920.11329645476510297No Hit
ATTGTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA7900.11301035260660525No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG7800.11157984181411656No Hit
CTCTTGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCCC7680.10986322886313016No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG7660.10957712670463242No Hit
ACCTTGCTTTAATCCTAATTGGCAAACTCCTTCTTTTCCTGACATTCATT7590.10857576914989034No Hit
CTACGGTACCTTGCTTTAATCCTAATTGGCAAACTCCTTCTTTTCCTGAC7550.10800356483289487No Hit
CTACTAAATATTTGCCCTTAGATAAAGGGATCAAACCGTATTATCCAGAG7530.10771746267439714No Hit
TTCAAGAACAGTTTCTCTCCCAAAAGTAAGACAGGAAATGTGAAACCACA7450.10657305404040619No Hit
GTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTGAG7440.10643000296115734No Hit
CCCTTAACTTCATGGGATATATAATTGGGAGTTGGGGCACATTGCCACAG7430.10628695188190847No Hit
GTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTC7330.10485644108941979No Hit
GTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATAC7290.10428423677242432No Hit
ATATATAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAA7210.10313982813843338No Hit
CAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTAT7170.10256762382143791No Hit
AAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGATAATA7170.10256762382143791No Hit
ACGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC7120.10185236842519359No Hit
ATTTAGTAGCATTGGGATAAAACCTAGCAGGCATAATTAATTTTAGTCTC7110.10170931734594471No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA7100.10156626626669585No Hit
CTTCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTA7050.10085101087045151No Hit
AGTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATA7030.10056490871195377No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT7000.10013575547420718No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)