FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences309159
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGCTTGGAAGTAATGATTAACTATGTGTTCAGG22590.7306919740327792No Hit
TCTATGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG20850.6744102549173726No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT14150.45769329050747354No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA13690.4428142153390327No Hit
GTGTAAAGGGAGCGGCAAAGCCTAGAAGCCCCACAATTCGCTGACAGACT12450.40270540401540955No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT12210.3949424082753535No Hit
CATTTAAACCCTACTAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT11770.38071024941858395No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG11050.3574212621984157No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC10280.3325149841990691No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9690.31343095300476453No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT9080.29370000549878866No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG8570.27720363955116945No Hit
GGATATTATCAATAGATGTCAACAATATGTTGGCCCTCTTACAATTAACG8480.2742925161486484No Hit
CTCTCACAATTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG8140.26329493885023564No Hit
TCCACAGAGTATGTAAATAATGCCTAGCTTGGAAGTAATGATTAACTATG8050.2603838154477146No Hit
GCCTAGCAGCCATGGAAAAGAGGTGTATTTCCGAGAGAGGACAACAGAGT7860.25423811048683687No Hit
GAGTATGTAAATAATGCCTAGCTTGGAAGTAATGATTAACTATGTGTTCA7630.24679857290261642No Hit
GTAGATCCCCAACCGGCCCCGGGACGGGTCGTCCGCAGGATTCAGCGCCG7590.24550474027927377No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7550.2442109076559311No Hit
TTGTAAGAGGGCCAACATATTGTTGACATCTATTGATAATATCCTCTTTC7500.24259361687675274No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7260.23483062113669664No Hit
GGTCTATTGATAGGAAGTTTTCGAAAACATTGTTTGATTTTGTGTACAAT7250.234507162980861No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC7240.2341837048250253No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAA7230.23386024666918964No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6680.21607004809822777No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAT6470.20927742682567868No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGCTT6320.20442555448814365No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG6320.20442555448814365No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGC6020.19472180981307352No Hit
ACAATATGTTGGCCCTCTTACAATTAACGAAAAAAGGAGATTAAAATTGA5740.18566498144967475No Hit
ACACAGAATAGCTTGCCTTAGTGCTGTATGGTGAGGTGAACAATGTTCCG5740.18566498144967475No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5660.1830773162029894No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5600.1811365672679754No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG5550.179519276488797No Hit
ATTATCAATAGATGTCAACAATATGTTGGCCCTCTTACAATTAACGAAAA5520.17854890202129No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5400.17466740415126197No Hit
CTCTTACAATTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG5380.17402048783959062No Hit
CAATATGTTCTTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATG5240.16949207365789123No Hit
ATTCAGAGCCAATTCAAACAATCCAGATTGGGACTTCAACCCCAACAAGG5190.1678747828787129No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA5090.1646402013203562No Hit
GAATTAGAGGACAAACGGGCAACATACCTTGGTAGTCCAGAAGAACCAAC5090.1646402013203562No Hit
ATGTAAGACCTTGGGCAAGACCTGTCGGGCGTTCACGGTGGTTTCCATGC5080.16431674316452052No Hit
CAATAGATGTCAACAATATGTTGGCCCTCTTACAATTAACGAAAAAAGGA4940.15978832898282114No Hit
CAATAGGTCTATTGATAGGAAGTTTTCGAAAACATTGTTTGATTTTGTGT4930.15946487082698546No Hit
AGTCTGTACAACATCTTGAAGCCCTTTTTACCGCTGTTACCAATTTTCTT4760.15396608217777907No Hit
TATCAATAGATGTCAACAATATGTTGGCCCTCTTACAATTAACGAAAAAA4660.15073150061942236No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAAGCCCTTTTTACC4640.15008458430775104No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC4630.14976112615191536No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4630.14976112615191536No Hit
GTACAAAGATCATTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA4600.14879075168440836No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA4590.14846729352857269No Hit
CCTGTCGGGCGTTCACGGTGGTTTCCATGCGACGTGCAGAGGTGAAGCGA4480.1449092538143803No Hit
GCCTAGAAGCCCCACAATTCGCTGACAGACTTTCCAATCAATAGGTCTAT4450.1439388793468733No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG4410.14264504672353062No Hit
GCACGGGACCATGCAAGACCTGCACGAATCCTGCTCAAGGAAACTCTACG4400.14232158856769495No Hit
GCCTTCCACAGAGTATGTAAATAATGCCTAGCTTGGAAGTAATGATTAAC4400.14232158856769495No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTAGTAGGGTTTAAATGTATA4390.14199813041185927No Hit
CATGTATACAAGCAAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4330.14005738147684527No Hit
GATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC4320.1397339233210096No Hit
ATGTTGGCCCTCTTACAATTAACGAAAAAAGGAGATTAAAATTGATTATG4280.13844009069766688No Hit
TCTTCTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCA4270.1381166325418312No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4240.1371462580743242No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA4230.13682279991848856No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC4200.13585242545098153No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4200.13585242545098153No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4160.13455859282763885No Hit
CCCACGTGTCATGGCCAAAATTTGCAGTCCCCAACCTCCAATCACTCACC4150.13423513467180317No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4150.13423513467180317No Hit
CCTTTACCCTGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG4140.13391167651596753No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4120.13326476020429617No Hit
CTTACAATTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGGTT4100.13261784389262482No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT4090.13229438573678914No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTTCTG4050.13100055311344647No Hit
ATTCTCTGTTGGGTTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA4030.13035363680177514No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT3980.1287363460225968No Hit
GGGGCTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGGCCGACCACGGGGC3970.1284128878667611No Hit
ATATGTTCTTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA3960.12808942971092543No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCAAAGCAGGCTTTCACTTT3900.12614868077591143No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTTCTGTACCTGTC3800.12291409921755471No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3780.12226718290588338No Hit
CCTTTTCGAGGTTTGGAAGACCAACCTCCCATGCTGTAGCTCTTGTTCCC3710.12000297581503368No Hit
CAACAATATGTTGGCCCTCTTACAATTAACGAAAAAAGGAGATTAAAATT3700.11967951765919803No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG3670.11870914319169101No Hit
TTATTATCCTGAACACATAGTTAATCATTACTTCCAAGCTAGGCATTATT3670.11870914319169101No Hit
GGGCTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGGCCGACCACGGGGCG3630.11741531056834832No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT3620.11709185241251265No Hit
GTACAACATCTTGAAGCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC3600.11644493610084132No Hit
CTTCTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC3550.11482764532166295No Hit
CAATAGACCTATTGATTGGAAAGTCTGTCAGCGAATTGTGGGGCTTCTAG3540.1145041871658273No Hit
GGTTTCCACTGTATGGCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGAT3530.11418072900999163No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA3490.11288689638664894No Hit
GCTTACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC3490.11288689638664894No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC3470.1122399800749776No Hit
CTTCTTCTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCT3470.1122399800749776No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC3420.11062268929579926No Hit
GCTCCGAATGCAGGGTCCAACTGGTGACCGGGAAAGAATCCCAGAGGATT3410.11029923113996358No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA3350.10835848220494956No Hit
GGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG3340.10803502404911389No Hit
GCTTGGAAGTAATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCC3330.10771156589327821No Hit
ATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGCTTGGA3290.10641773326993553No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATCATT3270.10577081695826418No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCACGAA3250.10512390064659285No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT3250.10512390064659285No Hit
AAATAATGCCTAGCTTGGAAGTAATGATTAACTATGTGTTCAGGATAATA3240.10480044249075718No Hit
TGCAAGACCTGCACGAATCCTGCTCAAGGAAACTCTACGTTTCCCTCTTG3240.10480044249075718No Hit
CCTCGACAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCTCTGGGATT3210.10383006802325016No Hit
GTTCCGGAACTGGAGCCACCAGCAGGTTTCCACTGTATGGCCTGAGGATG3200.10350660986741451No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTG3200.10350660986741451No Hit
GTGTACAATATGTTCTTGTGGTAAAGTACCCCAACTTCCAATTACATATC3200.10350660986741451No Hit
TCGACAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCTCTGGGATTCT3170.1025362353999075No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC3140.10156586093240048No Hit
GTACAATATGTTCTTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC3130.10124240277656481No Hit
ATGTAAATAATGCCTAGCTTGGAAGTAATGATTAACTATGTGTTCAGGAT3130.10124240277656481No Hit
ATTGATAGGAAGTTTTCGAAAACATTGTTTGATTTTGTGTACAATATGTT3130.10124240277656481No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGTCGGGCGTTC3120.10091894462072913No Hit
CTGATGGCCTATGGCCAAGCCCCAACCAGTGGGGGTTGCGTCAGCAAACA3100.1002720283090578No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)