FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences309159
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGCTTGGAAGTAATGATTAACTATGTGTTCAGG24220.7834156534339936No Hit
TCTATGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG19050.616187786866952No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA13310.43052280541727717No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT12900.41726102102801466No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT12180.39397203380784646No Hit
GTGTAAAGGGAGCGGCAAAGCCTAGAAGCCCCACAATTCGCTGACAGACT12050.3897670777819827No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG10560.3415718125624678No Hit
CATTTAAACCCTACTAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT10490.3393076054716182No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC9940.3215174069006563No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT9840.3182828253422996No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8880.28723084238207525No Hit
GGATATTATCAATAGATGTCAACAATATGTTGGCCCTCTTACAATTAACG8800.2846431771353899No Hit
GTAGATCCCCAACCGGCCCCGGGACGGGTCGTCCGCAGGATTCAGCGCCG8410.27202830905779873No Hit
TCCACAGAGTATGTAAATAATGCCTAGCTTGGAAGTAATGATTAACTATG8150.2636183970060713No Hit
CTCTCACAATTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG7980.25811960835686487No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7620.24647511474678077No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7510.2429170750325884No Hit
GAGTATGTAAATAATGCCTAGCTTGGAAGTAATGATTAACTATGTGTTCA7500.24259361687675274No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC7380.2387121190067247No Hit
GCCTAGCAGCCATGGAAAAGAGGTGTATTTCCGAGAGAGGACAACAGAGT7310.23644791191587503No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7270.23515407929253232No Hit
TTGTAAGAGGGCCAACATATTGTTGACATCTATTGATAATATCCTCTTTC7240.2341837048250253No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAA7080.2290083743316546No Hit
GGTCTATTGATAGGAAGTTTTCGAAAACATTGTTTGATTTTGTGTACAAT6900.22318612752661254No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6850.22156883674743416No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA6370.206042845267322No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGC6270.2028082637089653No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGCTT6120.19795639137143023No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAT6000.1940748935014022No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA5840.18889956300803146No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG5790.18728227222885313No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG5650.18275385804715372No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5410.17499086230709765No Hit
ACACAGAATAGCTTGCCTTAGTGCTGTATGGTGAGGTGAACAATGTTCCG5330.1724031970604123No Hit
ACAATATGTTGGCCCTCTTACAATTAACGAAAAAAGGAGATTAAAATTGA5270.17046244812539824No Hit
CTCTTACAATTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG5150.1665809502553702No Hit
CAATATGTTCTTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATG4890.15817103820364278No Hit
ATTATCAATAGATGTCAACAATATGTTGGCCCTCTTACAATTAACGAAAA4810.15558337295695743No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4780.1546129984894504No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA4690.15170187508692937No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4640.15008458430775104No Hit
GCACGGGACCATGCAAGACCTGCACGAATCCTGCTCAAGGAAACTCTACG4630.14976112615191536No Hit
CAATAGGTCTATTGATAGGAAGTTTTCGAAAACATTGTTTGATTTTGTGT4530.14652654459355866No Hit
AGTCTGTACAACATCTTGAAGCCCTTTTTACCGCTGTTACCAATTTTCTT4460.14426233750270895No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA4450.1439388793468733No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4430.14329196303520195No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC4390.14199813041185927No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4380.1416746722560236No Hit
GATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC4350.1407042977885166No Hit
ATGTAAGACCTTGGGCAAGACCTGTCGGGCGTTCACGGTGGTTTCCATGC4330.14005738147684527No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG4330.14005738147684527No Hit
TATCAATAGATGTCAACAATATGTTGGCCCTCTTACAATTAACGAAAAAA4320.1397339233210096No Hit
TCTTCTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCA4290.13876354885350256No Hit
GAATTAGAGGACAAACGGGCAACATACCTTGGTAGTCCAGAAGAACCAAC4240.1371462580743242No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT4200.13585242545098153No Hit
ATGTTGGCCCTCTTACAATTAACGAAAAAAGGAGATTAAAATTGATTATG4160.13455859282763885No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAAGCCCTTTTTACC4150.13423513467180317No Hit
ATATGTTCTTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA4100.13261784389262482No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA4100.13261784389262482No Hit
CATGTATACAAGCAAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4080.1319709275809535No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4050.13100055311344647No Hit
ATTCAGAGCCAATTCAAACAATCCAGATTGGGACTTCAACCCCAACAAGG4020.13003017864593946No Hit
CAATAGATGTCAACAATATGTTGGCCCTCTTACAATTAACGAAAAAAGGA4020.13003017864593946No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4000.1293832623342681No Hit
CCTTTACCCTGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG3990.12905980417843244No Hit
GCCTTCCACAGAGTATGTAAATAATGCCTAGCTTGGAAGTAATGATTAAC3960.12808942971092543No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTAGTAGGGTTTAAATGTATA3870.1251783063084044No Hit
GTACAAAGATCATTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA3850.12453138999673308No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG3840.1242079318408974No Hit
GCCTAGAAGCCCCACAATTCGCTGACAGACTTTCCAATCAATAGGTCTAT3830.12388447368506174No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT3770.1219437247500477No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCACGAA3760.12162026659421205No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3760.12162026659421205No Hit
CCTGTCGGGCGTTCACGGTGGTTTCCATGCGACGTGCAGAGGTGAAGCGA3750.12129680843837637No Hit
GTACAACATCTTGAAGCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC3740.12097335028254069No Hit
GTTTCTCTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATG3710.12000297581503368No Hit
GGTTTCCACTGTATGGCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGAT3650.11806222688001966No Hit
TTATTATCCTGAACACATAGTTAATCATTACTTCCAAGCTAGGCATTATT3630.11741531056834832No Hit
CCTTTTCGAGGTTTGGAAGACCAACCTCCCATGCTGTAGCTCTTGTTCCC3610.116768394256677No Hit
GGGGCTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGGCCGACCACGGGGC3600.11644493610084132No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC3590.11612147794500564No Hit
GGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG3590.11612147794500564No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCAAAGCAGGCTTTCACTTT3560.11515110347749863No Hit
CTTACAATTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGGTT3550.11482764532166295No Hit
CTTCTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC3540.1145041871658273No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTTCTG3530.11418072900999163No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC3490.11288689638664894No Hit
GCTTGGAAGTAATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCC3480.11256343823081327No Hit
CAATAGACCTATTGATTGGAAAGTCTGTCAGCGAATTGTGGGGCTTCTAG3470.1122399800749776No Hit
GGGCTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGGCCGACCACGGGGCG3450.11159306376330627No Hit
GAAGTTTTCGAAAACATTGTTTGATTTTGTGTACAATATGTTCTTGTGGT3450.11159306376330627No Hit
GTTCCGGAACTGGAGCCACCAGCAGGTTTCCACTGTATGGCCTGAGGATG3440.1112696056074706No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTTCTGTACCTGTC3440.1112696056074706No Hit
ATTCTCTGTTGGGTTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA3430.11094614745163492No Hit
GTGTACAATATGTTCTTGTGGTAAAGTACCCCAACTTCCAATTACATATC3420.11062268929579926No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT3390.10965231482829224No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTG3360.10868194036078524No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC3350.10835848220494956No Hit
GCTTACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC3340.10803502404911389No Hit
GTGTAAACAATATCTGAACCTTTACCCTGTTGCTCGGCAACGGTCAGGTC3340.10803502404911389No Hit
GTACAATATGTTCTTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC3310.10706464958160687No Hit
GTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAAC3300.1067411914257712No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC3300.1067411914257712No Hit
CTTCTTCTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCT3300.1067411914257712No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC3270.10577081695826418No Hit
CAACAATATGTTGGCCCTCTTACAATTAACGAAAAAAGGAGATTAAAATT3240.10480044249075718No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC3240.10480044249075718No Hit
ATGTAAATAATGCCTAGCTTGGAAGTAATGATTAACTATGTGTTCAGGAT3220.10415352617908584No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGCTTGGAAGTAATGATTAACTA3190.10318315171157884No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATCATT3190.10318315171157884No Hit
GCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGGCAAAGACCTGACC3180.10285969355574315No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT3180.10285969355574315No Hit
ATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGCTTGGA3150.10188931908823615No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC3140.10156586093240048No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)