FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences562767
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN53480.9503044776968087No Hit
GTATACATGCATATAAAGGCATCAAGGCGGGATAGCCACATTGTGTAAAA26680.4740860782526338No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG25650.45578365469190624No Hit
GTGTAAAAGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT25230.44832053052151244No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA19020.33797290885926146No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT18590.33033209125623925No Hit
CATGTATACAATCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC17990.31967048529853387No Hit
CATTTGAACCCTAATAAAACCAAACGCTGGGGTTACTCCCTTAACTTCAT17590.3125627479933969No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGCTGGGGTTACT16150.2869748936949039No Hit
GTATTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT15550.2763132877371985No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA15000.2665401489426352No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG14960.2658293752121216No Hit
GTTTTAATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA14770.2624531999921815No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG14700.26120934596378254No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTCTGTC14610.2596101050701267No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG14300.2541016086586456No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG14160.2516139006018477No Hit
GGAACATATTGTATTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAA13760.2445061632967107No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC13510.24006382748100014No Hit
CATATAAAGGCATCAAGGCGGGATAGCCACATTGTGTAAAAGGAGCAGCA13160.2338445573390053No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAATACAATA12980.2306460755516937No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT12810.2276252871970105No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG12550.2230052579486715No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC12530.22264987108341464No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG12410.22051754989187355No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC12300.21856292213296089No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAATACAATATGTT12200.21678598780667668No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC11930.2119882651257092No Hit
GTTCAAATGTATACCCAAAGACAGAAGAAAATTGGTAATAGAGGTAAAAA11590.2059466884163428No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG11520.20470283438794387No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11370.20203743289851753No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG11230.1995497248417196No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAAT11160.1983058708133206No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG11140.19795048394806378No Hit
CCCAAAGACAGAAGAAAATTGGTAATAGAGGTAAAAAGGGACTCAAGATG11040.19617354962177952No Hit
CCTTTTACACAATGTGGCTATCCCGCCTTGATGCCTTTATATGCATGTAT10870.19315276126709635No Hit
GTTAAGGGAGTAACCCCAGCGTTTGGTTTTATTAGGGTTCAAATGTATAC10810.19208660067132577No Hit
AGTTAAGGGAGTAACCCCAGCGTTTGGTTTTATTAGGGTTCAAATGTATA10620.18871042545138575No Hit
TAGCCACATTGTGTAAAAGGAGCAGCAAAGCCCAAAAGACCCACAATTCT10440.1855119436640741No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTATTAAAA10440.1855119436640741No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAATACAAT10430.1853342502314457No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG10220.1816026881462488No Hit
CTTTTTACCTCTATTACCAATTTTCTTCTGTCTTTGGGTATACATTTGAA10120.17982575381996457No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT9970.17716035233053823No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACGAGAAAAACCCC9820.17449495084111188No Hit
GTATACATTTGAACCCTAATAAAACCAAACGCTGGGGTTACTCCCTTAAC9760.1734287902453413No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT9730.17289570994745604No Hit
CTATTACCAATTTTCTTCTGTCTTTGGGTATACATTTGAACCCTAATAAA9630.1711187756211718No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC9600.17058569532328655No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA9560.16987492159277284No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAGAAGAAAATTGGTAAT9440.16774260040123176No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA9400.16703182667071806No Hit
ATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC9360.16632105294020438No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC9300.16525489234443383No Hit
CTTTACCACAGGAACATATTGTATTAAAACTCAAGCAATGTTTTCGGAAA9260.16454411861392015No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA9070.1611679433939801No Hit
GTGGGGGAAAGCCCTGCGAACCACTGAACAAATGGCACTAGTAAACTGAG8990.15974639593295273No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG8790.15619252728038424No Hit
GTAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTT8770.1558371404151274No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG8750.15548175354987054No Hit
GCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC8650.1537048192235863No Hit
GCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGCAGAGACCTGACC8610.1529940454930726No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAA8290.14730785564896307No Hit
ATCATATTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT8280.14713016221633463No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG8250.14659708191844936No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT8160.14499784102479357No Hit
TACATATCCCATGAAGTTAAGGGAGTAACCCCAGCGTTTGGTTTTATTAG8110.14410937386165143No Hit
TAATAAAACCAAACGCTGGGGTTACTCCCTTAACTTCATGGGATATGTAA8080.14357629356376617No Hit
CAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATG7960.14144397237222509No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC7940.14108858550696826No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGG7940.14108858550696826No Hit
GTACAAAACCTTCGGACGGAAACTGCACTTGTATTCCCATCCCATCATCC7890.14020011834382612No Hit
CTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTGCC7840.13931165118068403No Hit
GGATATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTATT7790.1384231840175419No Hit
GTTTAGATTGTATACATGCATATAAAGGCATCAAGGCGGGATAGCCACAT7750.1377124102870282No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG7600.13504700879760184No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCACCCATATCGTCAATCTTCTCG7580.13469162193234502No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTCTG7470.13273699417343235No Hit
CAATTACATATCCCATGAAGTTAAGGGAGTAACCCCAGCGTTTGGTTTTA7290.1295385123861207No Hit
GGATGATGGGATGGGAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTAC7280.1293608189534923No Hit
GTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTC7270.12918312552086386No Hit
CAATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTC7200.1279392714924649No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCAC7090.12598464373355225No Hit
ATCCTGGGCTTTCGCAAGATTCCTATGGGAGTGGGCCTCAGTCCGTTTCT6990.12420770940726802No Hit
TCCTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTG6950.12349693567675432No Hit
CTCCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCAC6850.12172000135047009No Hit
CTTCTGGACTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC6700.11905459986104373No Hit
GCCACATTGTGTAAAAGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTT6660.11834382613053004No Hit
GCCCAGGATGATGGGATGGGAATACAAGTGCAGTTTCCGTCCGAAGGTTT6590.11709997210213108No Hit
CCCATATCGTCAATCTTCTCGAGGACTGGGGACCCTGCACCGAACATGGA6580.11692227866950265No Hit
GACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGGGCTTT6560.11656689180424581No Hit
GGGTATACATTTGAACCCTAATAAAACCAAACGCTGGGGTTACTCCCTTA6550.11638919837161739No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT6520.11585611807373211No Hit
ATTTTCTTCTGTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGC6440.11443457061270472No Hit
ATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGT6420.11407918374744788No Hit
CTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTG6360.11301302315167734No Hit
CTTTATATGCATGTATACAATCTAAACAGGCTTTCACTTTCTCGCCAACT6320.11230224942116365No Hit
AATGTATACCCAAAGACAGAAGAAAATTGGTAATAGAGGTAAAAAGGGAC6310.11212455598853521No Hit
ATCCAGTGGGGGTTGCGTCAGCAAACACTTGGCAGAGACCTGACCGTTGC6260.11123608882539311No Hit
CACGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACCAA6230.11070300852750783No Hit
ATATTGTATTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGA6190.10999223479699415No Hit
GCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTACCAAGGTATGTTGC6180.1098145413643657No Hit
GGATAGCCACATTGTGTAAAAGGAGCAGCAAAGCCCAAAAGACCCACAAT6160.10945915449910887No Hit
GCCTGTAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTC6130.1089260742012236No Hit
AACATATTGTATTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAAT6020.10697144644231094No Hit
GAATATGATAAAACGCCGCAGACACATCCAGCGATAGCCAGGACAAATTG6020.10697144644231094No Hit
CATCCAGCGATAGCCAGGACAAATTGGAGGACAAGAGGTTGGTGAGTGAT5990.10643836614442567No Hit
ATTCCTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAG5970.10608297927916883No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCG5970.10608297927916883No Hit
GACCTGACCGTTGCCGGGCAACGGGGTAAAGGTGCAGGTATTGTTGACAC5970.10608297927916883No Hit
GGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCC5950.10572759241391197No Hit
ATATAAAGGCATCAAGGCGGGATAGCCACATTGTGTAAAAGGAGCAGCAA5930.10537220554865513No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5920.1051945121160267No Hit
ATGTTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG5910.10501681868339827No Hit
ATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTT5900.10483912525076987No Hit
ATCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTC5890.10466143181814143No Hit
CCTTTATATGCATGTATACAATCTAAACAGGCTTTCACTTTCTCGCCAAC5870.10430604495288459No Hit
CCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTT5860.10412835152025617No Hit
CTAATAAAACCAAACGCTGGGGTTACTCCCTTAACTTCATGGGATATGTA5850.10395065808762774No Hit
AATAAAACCAAACGCTGGGGTTACTCCCTTAACTTCATGGGATATGTAAT5810.10323988435711404No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTG5800.10306219092448562No Hit
ACCCTGCACCGAACATGGAGAGCACAACATCAGGATTCCTAGGACCCCTG5790.1028844974918572No Hit
GGATGAAGAGGAATATGATAAAACGCCGCAGACACATCCAGCGATAGCCA5790.1028844974918572No Hit
CCACTGAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGACTGAGG5780.10270680405922877No Hit
CTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATA5720.10164064346345823No Hit
CAATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCC5660.10057448286768769No Hit
AGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAATCT5630.10004140256980243No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)