FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences562767
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN53200.945329061583213No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG29030.5158440349203134No Hit
GTATACATGCATATAAAGGCATCAAGGCGGGATAGCCACATTGTGTAAAA27480.4883015528629077No Hit
GTGTAAAAGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT26560.47195375706109277No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA17850.31718277724173594No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT17730.3150504560501948No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGCTGGGGTTACT16880.2999465142767788No Hit
CATTTGAACCCTAATAAAACCAAACGCTGGGGTTACTCCCTTAACTTCAT16600.294971098163183No Hit
GTATTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT16560.29426032443266925No Hit
GTTTTAATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA16150.2869748936949039No Hit
CATGTATACAATCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC15760.2800448498223954No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA15630.2777348351982259No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG15010.26671784237526364No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTCTGTC14300.2541016086586456No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC14140.2512585137365908No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG13930.24752695165139393No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG13700.24344000270094018No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAATACAATA13490.23970844061574326No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC12880.22886914122540944No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG12710.22584835287072624No Hit
GGAACATATTGTATTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAA12480.2217614039202725No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAATACAATATGTT12260.2178521484024472No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG12200.21678598780667668No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC12200.21678598780667668No Hit
CATATAAAGGCATCAAGGCGGGATAGCCACATTGTGTAAAAGGAGCAGCA12020.21358750601936502No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG11970.21269903885622293No Hit
GTTCAAATGTATACCCAAAGACAGAAGAAAATTGGTAATAGAGGTAAAAA11730.20843439647314074No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG11690.20772362274262707No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC11490.20416975409005858No Hit
CCTTTTACACAATGTGGCTATCCCGCCTTGATGCCTTTATATGCATGTAT11280.2004381920048617No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT11270.20026049857223327No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT10840.19261968096921106No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAAT10750.19102044007555524No Hit
CTATTACCAATTTTCTTCTGTCTTTGGGTATACATTTGAACCCTAATAAA10660.18942119918189942No Hit
CTTTTTACCTCTATTACCAATTTTCTTCTGTCTTTGGGTATACATTTGAA10630.18888811888401416No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10410.18497886336618885No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG10370.18426808963567515No Hit
GTATACATTTGAACCCTAATAAAACCAAACGCTGGGGTTACTCCCTTAAC10300.18302423560727618No Hit
CCCAAAGACAGAAGAAAATTGGTAATAGAGGTAAAAAGGGACTCAAGATG10270.18249115530939092No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG10250.18213576844413407No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAATACAAT10240.18195807501150565No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTATTAAAA9800.174139563975855No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT9750.17325109681271292No Hit
GTTAAGGGAGTAACCCCAGCGTTTGGTTTTATTAGGGTTCAAATGTATAC9610.17076338875591499No Hit
ATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC9550.16969722816014443No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAGAAGAAAATTGGTAAT9540.16951953472751602No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG9450.1679202938338602No Hit
AGTTAAGGGAGTAACCCCAGCGTTTGGTTTTATTAGGGTTCAAATGTATA9410.1672095201033465No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA9370.1664987463728328No Hit
TAGCCACATTGTGTAAAAGGAGCAGCAAAGCCCAAAAGACCCACAATTCT9340.16596566607494753No Hit
CTTTACCACAGGAACATATTGTATTAAAACTCAAGCAATGTTTTCGGAAA9110.16187871712449378No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA9100.16170102369186537No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT9090.16152333025923696No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACGAGAAAAACCCC9080.16134563682660852No Hit
GTACAAAACCTTCGGACGGAAACTGCACTTGTATTCCCATCCCATCATCC8950.15903562220243903No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC8870.15761407474141162No Hit
GTTTAGATTGTATACATGCATATAAAGGCATCAAGGCGGGATAGCCACAT8870.15761407474141162No Hit
GCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGCAGAGACCTGACC8850.1572586878761548No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG8690.1544155929541No Hit
GGATATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTATT8640.1535271257909579No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG8520.15139480459941682No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC8430.149795563705761No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC8280.14713016221633463No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCACCCATATCGTCAATCTTCTCG8240.14641938848582095No Hit
GCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC8200.14570861475530728No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA8170.14517553445742198No Hit
CAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATG8110.14410937386165143No Hit
GTGGGGGAAAGCCCTGCGAACCACTGAACAAATGGCACTAGTAAACTGAG8090.1437539869963946No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG7910.140555505209083No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAA7850.13948934461331242No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTCTG7850.13948934461331242No Hit
CTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTGCC7610.13522470223023028No Hit
ATCATATTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT7600.13504700879760184No Hit
GTAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTT7480.1329146876060608No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCAC7460.1325593007408039No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGG7240.1286500452229786No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7210.12811696492509334No Hit
CTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTG7060.12545156343566696No Hit
CTCCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCAC6950.12349693567675432No Hit
TAATAAAACCAAACGCTGGGGTTACTCCCTTAACTTCATGGGATATGTAA6850.12172000135047009No Hit
ATATTGTATTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGA6850.12172000135047009No Hit
TCCTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTG6830.12136461448521323No Hit
GTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTC6830.12136461448521323No Hit
GACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGGGCTTT6800.12083153418732796No Hit
GGATGATGGGATGGGAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTAC6740.11976537359155742No Hit
GGGTATACATTTGAACCCTAATAAAACCAAACGCTGGGGTTACTCCCTTA6680.1186992129957869No Hit
CTTCTGGACTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC6550.11638919837161739No Hit
TACATATCCCATGAAGTTAAGGGAGTAACCCCAGCGTTTGGTTTTATTAG6520.11585611807373211No Hit
GCCACATTGTGTAAAAGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTT6510.11567842464110369No Hit
ATCCTGGGCTTTCGCAAGATTCCTATGGGAGTGGGCCTCAGTCCGTTTCT6490.11532303777584685No Hit
CCCATATCGTCAATCTTCTCGAGGACTGGGGACCCTGCACCGAACATGGA6490.11532303777584685No Hit
GCCCAGGATGATGGGATGGGAATACAAGTGCAGTTTCCGTCCGAAGGTTT6470.11496765091058998No Hit
CAATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTC6380.11336841001693418No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCG6370.11319071658430575No Hit
CACGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACCAA6270.11141378225802151No Hit
ATGTTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG6250.11105839539276467No Hit
CTTTATATGCATGTATACAATCTAAACAGGCTTTCACTTTCTCGCCAACT6150.10928146106648044No Hit
CAATTACATATCCCATGAAGTTAAGGGAGTAACCCCAGCGTTTGGTTTTA6140.10910376763385202No Hit
ATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGT6130.1089260742012236No Hit
ACCCTGCACCGAACATGGAGAGCACAACATCAGGATTCCTAGGACCCCTG6050.10750452674019621No Hit
ATTTTCTTCTGTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGC6010.10679375300968251No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA5970.10608297927916883No Hit
ATTCCTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAG5940.10554989898128354No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5880.104483738385513No Hit
GAATATGATAAAACGCCGCAGACACATCCAGCGATAGCCAGGACAAATTG5850.10395065808762774No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT5850.10395065808762774No Hit
GCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTACCAAGGTATGTTGC5830.1035952712223709No Hit
GGATAGCCACATTGTGTAAAAGGAGCAGCAAAGCCCAAAAGACCCACAAT5820.10341757778974246No Hit
GGATGAAGAGGAATATGATAAAACGCCGCAGACACATCCAGCGATAGCCA5800.10306219092448562No Hit
ATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTT5770.10252911062660036No Hit
ATCCAGTGGGGGTTGCGTCAGCAAACACTTGGCAGAGACCTGACCGTTGC5720.10164064346345823No Hit
GTTTTATCATATTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTG5690.10110756316557296No Hit
CCACTGAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGACTGAGG5660.10057448286768769No Hit
GACCTGACCGTTGCCGGGCAACGGGGTAAAGGTGCAGGTATTGTTGACAC5650.10039678943505927No Hit
ACCCTGTTCCGACTACTGCCTCACCCATATCGTCAATCTTCTCGAGGACT5630.10004140256980243No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)