FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489867

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489867
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences494068
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN44060.8917800788555422No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA35730.7231798052089996No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT16780.33962936275978206No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT14510.2936842701814325No Hit
GTATAATGTGATCTTGTGGCAATGATCCATAACAGCCAATGACATAGCCC12990.26291927426993855No Hit
GTGTAAAAGGGGCAGCAAACCCCAAAAGACCCACAATACGTTGACAGACT12790.2588712484921104No Hit
CATTTAAACCCTAACAAAACTAAACGATGGGGTTACTCTTTACATTTCAT12100.24490555955860327No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT11840.23964312604742669No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC10660.21575977395824056No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT10550.21353335978043508No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCTCCAAGGTCGGTCGTTGACA10370.20989013658038974No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC10250.20746132111369286No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT10110.20462770306921316No Hit
CCTCCAGCTTATAGACCACCTAATGCCCCTATCTTATCAACACTTCCGGA10020.20280609146919046No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG9930.20098447986916781No Hit
ATCCATAACAGCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCAT9880.19997247342471075No Hit
GTTATGGATCATTGCCACAAGATCACATTATACAGAAAATCAAAGAATGT9860.19956767084692795No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9650.1953172437802084No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCTTCAAGGTCGGTCGTTGACA9470.19167402058016306No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATT9420.19066201413570602No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAC9290.18803079738011771No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT9110.18438757418007237No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTC8870.1795299432466786No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG8100.1639450440020402No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC8090.16374264271314878No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA8040.16273063626869178No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA7980.1615162285353433No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA7760.15706340017973233No Hit
GTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATTCA7720.15625379502416673No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7670.1552417885797097No Hit
ACATTATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGG7580.15342017697968702No Hit
TCTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG7530.15240817053522995No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT7450.1507889602240987No Hit
ATTATGTGGTCTATAAGCTGGAGGAGTGCGAATCCACACTCCGAAAGACA7400.14977695377964168No Hit
ATCCAAATGTTACCAAATATTTGCCATTAGATAAGGGTATTAAACCTTAT7370.14916974991296744No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG7340.14856254604629324No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATTCATTTACA7220.14613373057959633No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAACCCCAAAAGACCCACAAT6990.14147850093509395No Hit
GCGTGGGACCATGCAGGACCTGCACGACTACTGTTCAAGGAACCTCTATG6950.14066889577952832No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTTCTGTGG6940.14046649449063692No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT6820.13803767902394No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC6640.1343944558238947No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG6510.1317632390683064No Hit
CATTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGTCCACT6510.1317632390683064No Hit
GAGTAACCCCATCGTTTAGTTTTGTTAGGGTTTAAATGTATACCCAAAGA6460.13075123262384936No Hit
GGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATT6270.12690560813491258No Hit
CCACCTAATGCCCCTATCTTATCAACACTTCCGGAGACTACTGTTGTTAG6110.12366718751265007No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATATGAAACCACA6090.12326238493486727No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC5860.1186071552903649No Hit
GGGTTACTCTTTACATTTCATGGGCTATGTCATTGGCTGTTATGGATCAT5850.11840475400147349No Hit
GTCCAAGAGTCCTCTTATGTAAGACAGAGAGTCCAAGAGTCCTCTTATGT5820.11779755013479926No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCATCATACTGCACTCAGGCA5720.11577353724588517No Hit
ACATTGACCCTTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCGTTT5700.11536873466810238No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT5610.11354712306807971No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5600.11334472177918829No Hit
GTCATTGGCTGTTATGGATCATTGCCACAAGATCACATTATACAGAAAAT5530.11192791275694844No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGACCCTTAT5520.11172551146805704No Hit
GCTTTATTCTTCCACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCT5500.1113207088902742No Hit
CCCCAAAAGACCCACAATACGTTGACAGACTTTCCAATCAATAGGCCTGT5400.10929669600136013No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5370.10868949213468591No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5350.1082846895569031No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG5310.10747508440133748No Hit
CTCTAAGGCTTCCCGATACAGAGCTGAGGCGGTATCAAGAAGATCTCGTA5240.10605827537909761No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC5240.10605827537909761No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5230.10585587409020622No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA5220.1056534728013148No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC5210.10545107151242339No Hit
CACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGTCCACTCACAGT5180.10484386764574917No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5170.10464146635685775No Hit
TCGCAGAAGATCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACT5160.10443906506796634No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5130.10383186120129213No Hit
CTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATTCATTTACACC5110.10342705862350932No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCATATTTCTT5090.1030222560457265No Hit
GCTCCAAGGTCGGTCGTTGACATTACAGAGAGTCCAAGAGTCCTCTTATG5010.10140304573459523No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG5000.10120064444570381No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACATAATCT4980.10079584186792102No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAAGCATACTTCAAA4980.10079584186792102No Hit
GGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAA4950.10018863800124679No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)