FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences545876
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAACCACACTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCTACAATACG90501.657885673669478No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCTACAATACGTTGACAGACT59741.0943877364090013No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACTACCGTG40350.7391788611332977No Hit
ATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAGTGGGC38990.714264778081469No Hit
GTGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATTCAATCCAAGC32810.6010522536253655No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT32250.5907935135452008No Hit
GATGTGAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGG31220.5719247594691835No Hit
GTTAATAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGTATGATGTGAT30990.5677113483648302No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGTATGATGTGAT29080.5327217170199826No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC25120.4601777693102463No Hit
GTCTGCACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTT24870.45559797463160134No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT23650.43324857659981386No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACACTGTGTAAAA21690.3973429863192373No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA20980.3843363694318856No Hit
CTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCA20930.3834204104961566No Hit
CCTATTAACAGGCCTATTGATTGGAAAGTCTGTCAACGTATTGTAGGTCT20530.3760927390103247No Hit
CTTTTACACAGTGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT20050.3672995332273264No Hit
AAACCACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC18500.3389048062197276No Hit
GGATAACCACACTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCTACAAT18220.33377543617964517No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG17920.32827968256527124No Hit
CAATAGGCCTGTTAATAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGT17600.32241754537660566No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA17480.3202192439308561No Hit
CGGTAGTTTCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC16820.30812858597923337No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC16520.3026328323648594No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT16310.29878580483479766No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16170.29622111981475646No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT15580.28541280437315436No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC15190.27826832467446816No Hit
TTGTTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGC14420.2641625570642417No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA14090.25811722808843035No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG13920.2550029677069518No Hit
CTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC13780.25243828268691054No Hit
ATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCCATGAA13430.24602657013680762No Hit
GGGTTACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCAT13310.24382826869105806No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT12690.23247037788801853No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCG12670.2321039943137269No Hit
CCCTCACAAAACAAAAAGATGGGGTTACTCTTTACATTTCATGGGCTATG12600.23082165180370634No Hit
ACAATACGTTGACAGACTTTCCAATCAATAGGCCTGTTAATAGGAAGTTT12530.22953930929368577No Hit
CATGCATACAAGGGCATTAAAGCAGGATAACCACACTGTGTAAAAGGGGC12120.22202844602070798No Hit
CCTCCAATTCCAGGATCTTCAACTACCAGCACGGGACCATGCAGAACCTG11740.21506715810916763No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA11520.2110369387919601No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC11250.20609076053902353No Hit
GACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAG11100.2033428837318365No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC11060.2026101165832533No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG11030.20206054122181594No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG10900.19967904798892055No Hit
GAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA10860.1989462808403374No Hit
ACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCA10860.1989462808403374No Hit
ACCCATAACATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCAT10800.19784713011746258No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC10650.19509925331027558No Hit
GCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGG10560.1934505272259634No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAAAAA10340.18942030790875586No Hit
CCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA10110.18520689680440247No Hit
AGCCAGAAAGGTTCCACGCATGCGCTGATGGCCCATGACCAAGCCCCAGC9950.18227582821006968No Hit
TAGCAGCAGGATGAAGAGGAAGATGATAAAACGCCGCAGACACATCCAGC9810.17971114319002848No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACA9770.17897837604144531No Hit
AGATCACATCATACAAAAAATCAAAGAATGTTTTAGAAAACTTCCTATTA9750.1786119924671537No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC9710.17787922531857053No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA9640.1765968828085499No Hit
TCCTATTAACAGGCCTATTGATTGGAAAGTCTGTCAACGTATTGTAGGTC9530.17458177314994613No Hit
GTTCAGGAACAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCA9510.17421538957565455No Hit
AATTAGAGGACAAACGGGCAACATACCTTGATAGTCCAGAAGAACCAACA9450.17311623885277974No Hit
GTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCC9280.17000197847130116No Hit
TTGACAGACTTTCCAATCAATAGGCCTGTTAATAGGAAGTTTTCTAAAAC9250.1694524031098638No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAAAAAATCAAAGAATGT9230.16908601953557217No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA9220.16890282774842638No Hit
GAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC9050.1657885673669478No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC9050.1657885673669478No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA8960.16413984128263562No Hit
CCTACTAACAGGCCTATTGATTGGAAAGTCTGTCAACGTATTGTAGGTCT8880.16267430698546922No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACT8870.16249111519832343No Hit
CACTTGGCACAGACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGT8830.16175834804974024No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC8800.16120877268830283No Hit
AGTCTGCACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT8610.15772812873253267No Hit
CATCATACAAAAAATCAAAGAATGTTTTAGAAAACTTCCTATTAACAGGC8520.15607940264822048No Hit
CTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTG8430.15443067656390827No Hit
ATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATGTATCCCTCCT8280.1516827997567213No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTAC8280.1516827997567213No Hit
GTTAGTAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGTATGATGTGAT8250.1511332243952839No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA8230.15076684082099231No Hit
GCATACAAGGGCATTAAAGCAGGATAACCACACTGTGTAAAAGGGGCAGC8080.14801896401380532No Hit
GCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGCACAGACCTGGCC8030.14710300507807633No Hit
ATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATAC7990.14637023792949316No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC7770.14234001861228557No Hit
CTTTCCAATCAATAGGCCTGTTAATAGGAAGTTTTCTAAAACATTCTTTG7770.14234001861228557No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG7730.14160725146370237No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACCACCGTG7700.141057676102265No Hit
TTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCT7700.141057676102265No Hit
ATCCTGGGCTTTCGGAAAATTCCTATGGGAGTGGGCCTCAGCCCGTTTCT7680.14069129252797338No Hit
GACCCATAACATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCA7670.1405081007408276No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTAGGTCTTTTGGGTTTT7660.1403249089536818No Hit
CATTAAAGCAGGATAACCACACTGTGTAAAAGGGGCAGCAAAACCCAAAA7660.1403249089536818No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG7660.1403249089536818No Hit
GCACAGACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTT7580.13885937465651538No Hit
TGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATGTATCCCTCCTG7550.138309799295078No Hit
AGCCAGAAAGGTTCCACGCATGCGCTGATGGCCCATGGCCAAGCCCCAGC7530.13794341572078642No Hit
AAACTACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC7510.1375770321464948No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG7480.13702745678505743No Hit
GTGCAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7470.1368442649979116No Hit
ACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCGAGGATT7440.13629468963647423No Hit
CAATGACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTTTG7320.13409638819072464No Hit
GCGCTGAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGT7280.13336362104214144No Hit
GTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTT7280.13336362104214144No Hit
GTCCCGTGCTGGTAGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC7250.13281404568070404No Hit
GGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG7240.13263085389355825No Hit
TCTGCACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTT7220.13226447031926664No Hit
CTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA7190.13171489495782926No Hit
CAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTGGATTGAA7180.13153170317068344No Hit
CCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCATGGGCTATG7180.13153170317068344No Hit
GCTGCACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCC7120.13043255244780866No Hit
GGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCT6950.12731829206633008No Hit
TTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCATGG6920.12676871670489268No Hit
GTCTCTCACATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAA6890.12621914134345527No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG6840.1253031824077263No Hit
GTGAAAGCCTGCTTGGATTGAATACATGCATACAAGGGCATTAAAGCAGG6840.1253031824077263No Hit
GGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATGTATCC6790.12438722347199731No Hit
ATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGA6700.12273849738768511No Hit
ATAACATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTT6660.12200573023910193No Hit
CCTCCTGTCCTCCAACTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGT6660.12200573023910193No Hit
GGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCC6660.12200573023910193No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA6580.12054019594193555No Hit
CTACAATACGTTGACAGACTTTCCAATCAATAGGCCTGTTAATAGGAAGT6540.11980742879335234No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC6510.11925785343191495No Hit
CCTCCTGTCCTCCAACTTGACCTGGTTATCGCTGGATGTGTCTGCGGCGT6510.11925785343191495No Hit
GACAAGAGGTTGGTGAGTGATTGGAGGTTGGGGACTGCGAATTTTGGCCA6450.11815870270904015No Hit
CCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTA6440.11797551092189436No Hit
CTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA6430.11779231913474855No Hit
CATGTATTCAATCCAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6400.11724274377331115No Hit
GACCTACAATACGTTGACAGACTTTCCAATCAATAGGCCTGTTAATAGGA6280.11504444232756157No Hit
TCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTT6110.111930181946083No Hit
TTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACA6100.1117469901589372No Hit
AGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAATTT6080.1113806065846456No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACTACC6060.11101422301035399No Hit
GGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACA6060.11101422301035399No Hit
CATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGT6060.11101422301035399No Hit
CTCTCACATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAACA6050.1108310312232082No Hit
GAATTAGAGGACAAACGGGCAACATACCTTGATAGTCCAGAAGAACCAAC6050.1108310312232082No Hit
CATATAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT6030.1104646476489166No Hit
GCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAGCCAGGAGAAA6010.110098264074625No Hit
ACCACACTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCTACAATACGTT5970.10936549692604182No Hit
GTAAAAAGGGACTCAAGATGCTGTACAGACTTGGCCCCCAATACCACATC5920.10844953799031283No Hit
TCGTAGGGCTTTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGG5860.10735038726743802No Hit
CTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAA5830.10680081190600063No Hit
CAATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCC5800.10625123654456324No Hit
ATTCACATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAGTGGGC5720.10478570224739683No Hit
CTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTC5690.10423612688595944No Hit
CTCACATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAACATG5670.10386974331166784No Hit
TTGTCTGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGC5660.10368655152452205No Hit
GATTGAATACATGCATACAAGGGCATTAAAGCAGGATAACCACACTGTGT5560.10185463365306408No Hit
GTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAAAAAAT5530.10130505829162666No Hit
ACCCAAAAGACCTACAATACGTTGACAGACTTTCCAATCAATAGGCCTGT5530.10130505829162666No Hit
GTATGCATGTATTCAATCCAAGCAGGCTTTCACTTTCTCGCCAACTTACA5510.10093867471733507No Hit
ATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACCTG5510.10093867471733507No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC5470.10020590756875188No Hit
GTTTTCCTTGTTGACAAGAATCCTCACAATACCGCAGAGTCTAGACTCGT5460.10002271578160608No Hit
TCACAATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTA5460.10002271578160608No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)