FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489873

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489873
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences545876
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAACCACACTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCTACAATACG72351.3253925799998534No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCTACAATACGTTGACAGACT55331.013600158277704No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACTACCGTG43380.7946859726384746No Hit
ATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAGTGGGC36420.6671844887849988No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT32850.6017850207739487No Hit
GTGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATTCAATCCAAGC30820.5645970879833515No Hit
GTTAATAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGTATGATGTGAT29350.5376678952729191No Hit
GTCTGCACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTT28310.5186159494097561No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGTATGATGTGAT27450.5028614557152175No Hit
GATGTGAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGG25960.4755658794304934No Hit
CCTATTAACAGGCCTATTGATTGGAAAGTCTGTCAACGTATTGTAGGTCT24950.4570635089287677No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT24520.44918626208149837No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC22180.40631938388938144No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA20550.3764591225846163No Hit
CTTTTACACAGTGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT20160.36931464288593013No Hit
GGATAACCACACTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCTACAAT18730.34311821732408093No Hit
CTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCA18610.3409199158783313No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACACTGTGTAAAA18010.32992840864958345No Hit
AAACCACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC17370.3182041342722523No Hit
CAATAGGCCTGTTAATAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGT17340.3176545589108149No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG16760.30702943525635856No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC16310.29878580483479766No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16040.2938396265818611No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA15620.28614557152173753No Hit
CCCTCACAAAACAAAAAGATGGGGTTACTCTTTACATTTCATGGGCTATG14930.27350533820867745No Hit
CTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC14810.27130703676292783No Hit
GGGTTACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCAT14160.2593995705984509No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCG14010.25665169379126396No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC13880.2542702005583686No Hit
TTGTTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGC13820.25317104983549377No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA13760.25207189911261896No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT13710.25115594017688997No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT13650.2500567894540152No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT13530.24785848800826563No Hit
CGGTAGTTTCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC12890.2361342136309345No Hit
ATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCCATGAA12580.23045526822941476No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG12160.2227612131692912No Hit
CCTCCAATTCCAGGATCTTCAACTACCAGCACGGGACCATGCAGAACCTG11980.2194637610006668No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC11880.21763184312920883No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC11780.21579992525775082No Hit
ACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCA11510.2108537470048143No Hit
ACAATACGTTGACAGACTTTCCAATCAATAGGCCTGTTAATAGGAAGTTT11430.20938821270764787No Hit
TCCTATTAACAGGCCTATTGATTGGAAAGTCTGTCAACGTATTGTAGGTC11400.20883863734621047No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA11290.2068235276876067No Hit
GAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA11280.2066403359004609No Hit
CCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA10870.19912947262748318No Hit
GTTCAGGAACAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCA10810.1980303219046084No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTAGGTCTTTTGGGTTTT10670.1954656368845672No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC10520.19271776007738023No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC10510.1925345682902344No Hit
CATGCATACAAGGGCATTAAAGCAGGATAACCACACTGTGTAAAAGGGGC10250.18777158182444365No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACT10170.18630604752727728No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACA10110.18520689680440247No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAAAAAATCAAAGAATGT10090.18484051323011086No Hit
AGATCACATCATACAAAAAATCAAAGAATGTTTTAGAAAACTTCCTATTA9890.1811766774871949No Hit
ACCCATAACATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCAT9880.1809934857000491No Hit
GACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAG9830.18007752676432012No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAAAAA9820.1798943349771743No Hit
CATCATACAAAAAATCAAAGAATGTTTTAGAAAACTTCCTATTAACAGGC9740.1784288006800079No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA9550.17494815672423775No Hit
CCTACTAACAGGCCTATTGATTGGAAAGTCTGTCAACGTATTGTAGGTCT9430.17274985527848816No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG9420.17256666349134236No Hit
GCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGG9080.1663381427283852No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC9060.1659717591540936No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC8840.16194153983688603No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA8700.15937685481684485No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACCACCGTG8690.15919366302969906No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG8620.15791132051967846No Hit
ATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATAC8580.15717855337109526No Hit
CTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTG8550.15662897800965786No Hit
AGTCTGCACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT8520.15607940264822048No Hit
GAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC8420.15424748477676248No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTAC8370.1533315258410335No Hit
GTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTT8290.15186599154386712No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA8270.1514996079695755No Hit
GTGCAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA8180.14985088188526333No Hit
CCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCATGGGCTATG8170.14966769009811753No Hit
AGCCAGAAAGGTTCCACGCATGCGCTGATGGCCCATGACCAAGCCCCAGC8090.1482021558009511No Hit
CACTTGGCACAGACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGT8010.14673662150378475No Hit
ATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATGTATCCCTCCT7940.14545427899376415No Hit
CTTTCCAATCAATAGGCCTGTTAATAGGAAGTTTTCTAAAACATTCTTTG7910.14490470363232674No Hit
CTCTCACATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAACA7900.14472151184518095No Hit
GTTAGTAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGTATGATGTGAT7890.14453832005803516No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG7860.14398874469659775No Hit
GCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGCACAGACCTGGCC7840.14362236112230617No Hit
ACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCGAGGATT7820.14325597754801458No Hit
AAACTACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC7790.14270640218657718No Hit
GTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCC7750.14197363503799396No Hit
TTGACAGACTTTCCAATCAATAGGCCTGTTAATAGGAAGTTTTCTAAAAC7730.14160725146370237No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG7720.14142405967655658No Hit
AATTAGAGGACAAACGGGCAACATACCTTGATAGTCCAGAAGAACCAACA7660.1403249089536818No Hit
ATCCTGGGCTTTCGGAAAATTCCTATGGGAGTGGGCCTCAGCCCGTTTCT7580.13885937465651538No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA7530.13794341572078642No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC7460.13666107321076582No Hit
TAGCAGCAGGATGAAGAGGAAGATGATAAAACGCCGCAGACACATCCAGC7440.13629468963647423No Hit
CTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA7380.13519553891359942No Hit
TCTGCACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTT7340.13446277176501623No Hit
GGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG7220.13226447031926664No Hit
GTCTCTCACATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAA7210.13208127853212084No Hit
GCGCTGAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGT7140.13079893602210027No Hit
GGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCT7060.12933340172493385No Hit
CCTCCTGTCCTCCAACTTGACCTGGTTATCGCTGGATGTGTCTGCGGCGT7030.12878382636349647No Hit
CATTAAAGCAGGATAACCACACTGTGTAAAAGGGGCAGCAAAACCCAAAA7000.12823425100205907No Hit
GCACAGACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTT6990.12805105921491328No Hit
TTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCATGG6890.12621914134345527No Hit
CCTCCTGTCCTCCAACTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGT6870.1258527577691637No Hit
CATGTATTCAATCCAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6770.12402083989770571No Hit
CAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTGGATTGAA6750.12365445632341412No Hit
GCATACAAGGGCATTAAAGCAGGATAACCACACTGTGTAAAAGGGGCAGC6680.12237211381339351No Hit
GTCCCGTGCTGGTAGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC6670.12218892202624772No Hit
CCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTA6670.12218892202624772No Hit
GACCCATAACATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCA6620.12127296309051873No Hit
GGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACA6600.12090657951622713No Hit
CTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA6530.11962423700620653No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG6530.11962423700620653No Hit
ATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGA6440.11797551092189436No Hit
CAATGACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTTTG6430.11779231913474855No Hit
TGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATGTATCCCTCCTG6420.11760912734760276No Hit
CTGCCGCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTATCAAGGTAT6400.11724274377331115No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC6380.11687636019901955No Hit
CTACAATACGTTGACAGACTTTCCAATCAATAGGCCTGTTAATAGGAAGT6370.11669316841187376No Hit
TTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCT6370.11669316841187376No Hit
GACCTACAATACGTTGACAGACTTTCCAATCAATAGGCCTGTTAATAGGA6300.11541082590185317No Hit
AGCCAGAAAGGTTCCACGCATGCGCTGATGGCCCATGGCCAAGCCCCAGC6260.11467805875326999No Hit
GTATGCATGTATTCAATCCAAGCAGGCTTTCACTTTCTCGCCAACTTACA6210.11376209981754097No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA6180.11321252445610358No Hit
GCTGCACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCC6160.11284614088181198No Hit
GCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAGCCAGGAGAAA6150.11266294909466619No Hit
GGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCC6120.1121133737332288No Hit
TCGTAGGGCTTTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGG6100.1117469901589372No Hit
TCACAATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTA6070.11119741479749981No Hit
GGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATGTATCC6040.11064783943606239No Hit
CTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTC6010.110098264074625No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG6000.10991507228747921No Hit
CTCACATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAACATG6000.10991507228747921No Hit
ACCCAAAAGACCTACAATACGTTGACAGACTTTCCAATCAATAGGCCTGT5990.1097318805003334No Hit
ATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACCTG5930.10863272977745862No Hit
GTTTTCCTTGTTGACAAGAATCCTCACAATACCGCAGAGTCTAGACTCGT5920.10844953799031283No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC5900.10808315441602123No Hit
GCTTTAATGCCCTTGTATGCATGTATTCAATCCAAGCAGGCTTTCACTTT5870.10753357905458381No Hit
TCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTT5820.10661762011885483No Hit
CAATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCC5800.10625123654456324No Hit
CTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTG5760.10551846939598004No Hit
GACAAGAGGTTGGTGAGTGATTGGAGGTTGGGGACTGCGAATTTTGGCCA5740.10515208582168845No Hit
CTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAA5730.10496889403454265No Hit
TTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACA5720.10478570224739683No Hit
GCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTA5640.10332016795023045No Hit
GTGAAAGCCTGCTTGGATTGAATACATGCATACAAGGGCATTAAAGCAGG5630.10313697616308466No Hit
AGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAATTT5580.10222101722735567No Hit
ACCACACTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCTACAATACGTT5570.10203782544020987No Hit
GGGCATTAAAGCAGGATAACCACACTGTGTAAAAGGGGCAGCAAAACCCA5560.10185463365306408No Hit
ATTCACATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAGTGGGC5540.10148825007877246No Hit
CATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGT5490.10057229114304347No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC5460.10002271578160608No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)